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Literature summary for 3.1.1.4 extracted from

  • Hoffman, W.J.; Vahey, M.; Hajdu, J.
    Pancreatic porcine phospholipase A2 catalyzed hydrolysis of phosphatidylcholine in lecithin-bile salt mixed micelles: Kinetic studies in a lecithin-sodium cholate system (1983), Arch. Biochem. Biophys., 221, 361-370.
    View publication on PubMed
Search for more literature for 3.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
additional information mixed micelle required to bind to the enzyme before the substrate, not altered by reaction Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Naja naja
-
 ?
-
 ?
phospholipids + H2O Sus scrofa aldosterone-dependent sodium transport ?
-
 ?
phospholipids + H2O Sus scrofa cardiac contraction and excitation ?
-
 ?
phospholipids + H2O Sus scrofa release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Naja naja
-
 cobra
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1 10 phospholipid/Triton X-100: 1/2,1/3,und 1/4, phospholipid concentration up to 30 mM Sus scrofa
82
-
 5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.20 Sus scrofa
150
-
 5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.36 Sus scrofa
330
-
 freshly dialyzed PLA2 Sus scrofa
383
-
 5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67 Sus scrofa
460
-
 7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67 Sus scrofa
520
-
 10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67 Sus scrofa
550
-
 12.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67 Sus scrofa
550
-
 15 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67 Sus scrofa
576
-
 5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0 Sus scrofa
741
-
 5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42 Sus scrofa
775
-
 7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0 Sus scrofa
804
-
 10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0 Sus scrofa
870
-
 7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42 Sus scrofa
900
-
 12.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0 Sus scrofa
900
-
 15 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0 Sus scrofa
933
-
 5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 2.0 Sus scrofa
1050
-
 10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42 Sus scrofa
1100
-
 12.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42 Sus scrofa
1100
-
 phospholipid/Triton X-100: 1/4 Naja naja
1100
-
 15 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42 Sus scrofa
1670
-
 phospholipid/Triton X-100: 1/3 Naja naja
2100
-
 phospholipid/Triton X-100: 1/2 Naja naja

Storage Stability

Storage Stability Organism
4°C, pH 7.5, 0.05 M sodium phosphate buffer, no loss of activity over a period of several months Naja naja

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Sus scrofa 1-acylglycerophosphocholine + fatty acid
-
 ?
phospholipids + H2O
-
 Naja naja ?
-
 ?
phospholipids + H2O aldosterone-dependent sodium transport Sus scrofa ?
-
 ?
phospholipids + H2O cardiac contraction and excitation Sus scrofa ?
-
 ?
phospholipids + H2O release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins Sus scrofa ?
-
 ?