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Literature summary for 3.1.1.4 extracted from
- The purification and characterization of a phospholipase A in hamster heart cytosol for the hydrolysis of phosphatidylcholine (1987), J. Biol. Chem., 262, 16927-16935.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| anti-peptide antibody | - |
Mesocricetus auratus | |
| Sodium dodecyl sulfate | - |
Mesocricetus auratus |  |
| Triton X-100 | - |
Mesocricetus auratus | |
| Tween 20 | - |
Mesocricetus auratus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
1-palmitoyl-2-arachidonoylphosphatidylcholine | - |
Mesocricetus auratus | |
| 0.5 | - |
1-stearoyl-2-arachidonoylphosphatidylcholine | - |
Mesocricetus auratus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mesocricetus auratus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
SDS-PAGE, Mr of one subunit | Mesocricetus auratus |
| 140000 | - |
gel filtration, multimeric complex | Mesocricetus auratus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Mesocricetus auratus | cardiac contraction and excitation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesocricetus auratus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange chromatography, gel-filtration chromatography,167fold | Mesocricetus auratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Mesocricetus auratus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0192 | - |
substrate: phosphatidylcholine | Mesocricetus auratus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM Hepes, 5 mM EDTA, 48 hours without loss of activity, 75% of the initial activity remains after one week, storage at -70°C does not improve the enzymes stability | Mesocricetus auratus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O | - |
Mesocricetus auratus | 1-palmitoyl-glycerophosphorylcholine + arachidonic acid | - |
? | |
| 1-palmitoyl-2-linoleoylphosphatidylcholine + H2O | - |
Mesocricetus auratus | 1-palmitoyl-glycerophosphorylcholine + linolic acid | - |
? | |
| 1-stearoyl-2-arachidonoylphosphatidylcholine + H2O | - |
Mesocricetus auratus | 1-stearoyl-glycerophosphorylcholine + arachidonic acid | - |
? | |
| phosphatidylcholine + H2O | - |
Mesocricetus auratus | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| phosphatidylethanolamine + H2O | - |
Mesocricetus auratus | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
| phospholipids + H2O | cardiac contraction and excitation | Mesocricetus auratus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 14000, identical subunits, at least six subunits could be cross-linked | Mesocricetus auratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
substrate: 1-palmitoyl-2-arachidonoylphosphatidylcholine | Mesocricetus auratus |
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Release 2023.1 (January 2023)
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