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Literature summary for 3.1.1.4 extracted from
- Purification and characterization of guinea pig gastric phospholipase A2 of the pancreatic type (1993), Eur. J. Biochem., 215, 81-90.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| anti-peptide antibody | - |
Cavia porcellus | |
| EGTA | - |
Cavia porcellus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| secretory granule | - |
Cavia porcellus | 30141 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13900 | - |
HPLC-electrospray mass spectrometry | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Cavia porcellus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| reverse-phase HPLC, gel filtration, from gastric juice, 180fold | Cavia porcellus |
| reverse-phase HPLC, gel filtration, from gastric mucosa, 51.5fold | Cavia porcellus |
| reverse-phase HPLC, gel filtration, from pancreas, more than 34.7fold | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Cavia porcellus | - |
| stomach | gastric mucosa and juices | Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.12 | - |
pancreas, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate | Cavia porcellus |
| 0.211 | - |
pancreas, substrate: 1-O-hexadecyl-oleolyl-sn-glycero-3-phosphocholine | Cavia porcellus |
| 0.45 | - |
antrum, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate | Cavia porcellus |
| 8.4 | - |
gastric juice, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate | Cavia porcellus |
| 20 | - |
fundus, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate | Cavia porcellus |
| 1000 | - |
gastric juice, substrate: 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine | Cavia porcellus |
| 1030 | - |
gastric mucosa, substrate: 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-hexadecyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O | - |
Cavia porcellus | 1-O-hexadecyl-sn-glycero-3-phosphocholine + oleate | - |
? | |
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine + H2O | - |
Cavia porcellus | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol + H2O | - |
? | |
| phospholipids + H2O | - |
Cavia porcellus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | gastric PLA2, no activity detected below pH 5.0 | Cavia porcellus |
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