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Literature summary for 3.1.1.4 extracted from

  • Liu, X.; Pan, H.; Yang, G.; Wu, X.; Zhou, Y.
    Cloning, expression and biochemical characterization of a basic-acidic hybrid phospholipase A2-II from Agkistrodon halys Pallas (1999), Biochim. Biophys. Acta, 1431, 157-165.
    View publication on PubMed
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
basic-acidic hybrid PLA2 cDNA, cloned into the expression vector pBLMVL2 for later transformation in Escherichia coli Gloydius halys

Crystallization (Commentary)

Crystallization (Comment) Organism
acidic and basic PLA2 Gloydius halys

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Gloydius halys
-
 lysophosphatidylcholine + a fatty acid
-
 ?
phospholipids + H2O Gloydius halys myotoxicity ?
-
 ?
phospholipids + H2O Gloydius halys presynaptic neurotoxicity of neutral PLA2 ?
-
 ?
phospholipids + H2O Gloydius halys cardiac toxicity ?
-
 ?
phospholipids + H2O Gloydius halys hemolytic activity of basic PLA2 ?
-
 ?
phospholipids + H2O Gloydius halys influence on platelet aggregation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gloydius halys
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gloydius halys

Renatured (Commentary)

Renatured (Comment) Organism
basic-acidic hybrid PLA2 Gloydius halys

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Gloydius halys
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
500
-
 de-/renatured basic PLA2, PC for substrate Gloydius halys
650
-
 de-/renatured basic-acidic PLA2, PC for substrate Gloydius halys
750
-
 de-/renatured acidic PLA2, PA for substrate Gloydius halys
1500
-
 de-/renatured acidic PLA2, PC for substrate Gloydius halys
1600
-
 de-/renatured basic-acidic PLA2, PA for substrate Gloydius halys
1750
-
 de-/renatured basic PLA2, PA for substrate Gloydius halys

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Gloydius halys 1-acylglycerophosphocholine + fatty acid
-
 ?
phosphatidylcholine + H2O
-
 Gloydius halys lysophosphatidylcholine + a fatty acid
-
 ?
phospholipids + H2O myotoxicity Gloydius halys ?
-
 ?
phospholipids + H2O presynaptic neurotoxicity of neutral PLA2 Gloydius halys ?
-
 ?
phospholipids + H2O cardiac toxicity Gloydius halys ?
-
 ?
phospholipids + H2O hemolytic activity of basic PLA2 Gloydius halys ?
-
 ?
phospholipids + H2O influence on platelet aggregation Gloydius halys ?
-
 ?