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Literature summary for 3.1.1.32 extracted from

  • Xin, R.; Khan, F.I.; Zhao, Z.; Zhang, Z.; Yang, B.; Wang, Y.
    A comparative study on kinetics and substrate specificities of phospholipase A1 with Thermomyces lanuginosus lipase (2017), J. Colloid Interface Sci., 488, 149-154 .
    View publication on PubMed
Search for more literature for 3.1.1.32

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.36
-
 phosphatidylcholine pH 7.0, 25°C Thermomyces lanuginosus
1.53
-
 triacylglycerol pH 7.0, 25°C Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation fusion protein of Thermomyces lanuginosus lipase and an extra C-terminal region of Fusarium oxysporum lipase Thermomyces lanuginosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
477
-
 substrate tributyrin, pH 7.0, 25°C Thermomyces lanuginosus
1463
-
 substrate triolein, pH 7.0, 25°C Thermomyces lanuginosus
3092
-
 substrate trioctanoin, pH 7.0, 25°C Thermomyces lanuginosus
4403
-
 substrate soy bean phosphatidylcholine, pH 7.0, 25°C Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Thermomyces lanuginosus 2-acylglycerophosphocholine + a carboxylate
-
 ?
triacylglycerol + H2O
-
 Thermomyces lanuginosus 1,2-diacylglycerol + a carboxylate
-
 ?
tributyrin + H2O
-
 Thermomyces lanuginosus butyric acid + 1,2-dibutyrylglycerol
-
 ?
trioctanoin + H2O
-
 Thermomyces lanuginosus octanoate + 1,2-dioctanoylglycerol
-
 ?
triolein + H2O
-
 Thermomyces lanuginosus oleate + 1,2-dioleylglycerol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2270
-
 triacylglycerol pH 7.0, 25°C Thermomyces lanuginosus
5158
-
 phosphatidylcholine pH 7.0, 25°C Thermomyces lanuginosus

General Information

General Information Comment Organism
physiological function the C-terminal region regulates the binding of PLA1 to phosphatidylcholine. The alpha9 helix of C-terminal region in PLA1 may not only control the opening of lid but also serve as a membrane anchor that assists in binding to phosphatidylcholine Thermomyces lanuginosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1488
-
 triacylglycerol pH 7.0, 25°C Thermomyces lanuginosus
3780
-
 phosphatidylcholine pH 7.0, 25°C Thermomyces lanuginosus