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Literature summary for 3.1.1.32 extracted from

  • Belaunzaran, M.L.; Wilkowsky, S.E.; Lammel, E.M.; Gimenez, G.; Bott, E.; Barbieri, M.A.; de Isola, E.L.
    Phospholipase A1: a novel virulence factor in Trypanosoma cruzi (2013), Mol. Biochem. Parasitol., 187, 77-86.
    View publication on PubMed
Search for more literature for 3.1.1.32

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid equence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS, the enzyme is mainly localized in inclusion bodies Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme is a membrane-bound/secreted enzyme in the infective stages of Trypanosoma cruzi Trypanosoma cruzi 16020
-
additional information immunohistochemic analysis of subcellular localization Trypanosoma cruzi
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi H2EIY3 strains Cvd and RA (lethal) and K98 (non-lethal)
-

Purification (Commentary)

Purification (Comment) Organism
soluble portion of recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
amastigote infective Trypanosoma cruzi
-
epimastigote
-
 Trypanosoma cruzi
-
trypomastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dipalmitoyl-phosphatidylcholine + H2O substrate from Glycine max Trypanosoma cruzi 2-palmitoyl-sn-glycerophosphocholine + palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
PLA1
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 assay at Trypanosoma cruzi

Expression

Organism Comment Expression
Trypanosoma cruzi increased levels of PLA1 expression in the infective amastigotes and trypomastigotes compared to the non-infective epimastigotes of lethal RA strain up

General Information

General Information Comment Organism
physiological function lysophospholipase activity in live Tryypanosoma cruzi parasites contributes to self protection against toxic compounds generated by phospholipase A1 activity Trypanosoma cruzi