Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
expression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D148N | 100% of wild-type activity | Escherichia coli |
D149A | 100% of wild-type activity | Escherichia coli |
D149N | approx. 85% of wild-type activity | Escherichia coli |
D184A | approx. 30% of wild-type activity | Escherichia coli |
H26C | introduced cysteine covalently links 2 OMPLA monomers to an active dimer via disulfide bond | Escherichia coli |
S152N | approx. 6% of wild-type activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | no activity in the presence of EDTA | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
integral to membrane | - |
Escherichia coli | - |
- |
outer membrane | - |
Escherichia coli | 19867 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for actiivty, one Ca2+ per monomer bound at the dimer interface | Escherichia coli | |
Ca2+ | required for activity, only in dimeric OMPLA high affinity Ca2+-sites are formed that are essential for catalysis | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE, OMPLA is only active in the dimeric state | Escherichia coli |
31000 | - |
2 * 31000, OMPLA is only active as a dimer | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | Escherichia coli | - |
2-acylglycerophosphocholine + a carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant OMPLA | Escherichia coli |
recombinant phospholipase A | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84 | - |
substrate hexadecanoylthioethane-1-phosphocholine | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dioctadecenoylthio-sn-glycero-3-phosphocholine + H2O | - |
Escherichia coli | 1-thio-2-octadecenoylthio-sn-glycero-3-phosphocholine + octadecenoate | - |
? | |
1,2-dioctadecenoylthio-sn-glycero-3-phosphoethanolamine + H2O | - |
Escherichia coli | 1-thio-2-octadecenoylthio-sn-glycero-3-phosphoethanolamine + octadecenoate | - |
? | |
1,2-dioctadecenoylthio-sn-glycero-3-phosphoglycerol + H2O | - |
Escherichia coli | 1-thio-2-octadecenoylthio-sn-glycero-3-phosphoglycerol + octadecenoate | - |
? | |
2-hexadecanoylthio-ethane-1-phosphocholine + H2O | - |
Escherichia coli | thio-ethane-1-phosphocholine + hexadecanoate | - |
? | |
hexadecanoylthioethane-1-phosphocholine + H2O | - |
Escherichia coli | hexadecanoate + thioethane-1-phosphocholine | - |
? | |
phosphatidylcholine + H2O | - |
Escherichia coli | 2-acylglycerophosphocholine + a carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27000, SDS-PAGE, OMPLA is only active in the dimeric state | Escherichia coli |
dimer | 2 * 31000, OMPLA is only active as a dimer | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
OMPLA | - |
Escherichia coli |
outer membrane phospholipase A | - |
Escherichia coli |