Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rabbit anti-phospholipase A antibodies | - |
Mesocricetus auratus | |
SDS | - |
Mesocricetus auratus | |
Triton X-100 | - |
Mesocricetus auratus | |
Tween 20 | - |
Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
1-palmitoyl-2-arachidonoylglycerophosphocholine | - |
Mesocricetus auratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mesocricetus auratus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 10% activation | Mesocricetus auratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
x * 14000, at least six subunits of the native enzyme can be cross-linked, SDS-PAGE | Mesocricetus auratus |
140000 | - |
gel filtration | Mesocricetus auratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mesocricetus auratus | the enzyme is important in phospholipid catabolism and the remodeling of the acyl groups of phospholipids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Mesocricetus auratus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.155 | - |
- |
Mesocricetus auratus |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Hepes, 5 mM EDTA, pH 7.4, stable for 48 h, 25% loss of activity after 1 week | Mesocricetus auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-palmitoyl-2-arachidonoylglycerophosphocholine + H2O | - |
Mesocricetus auratus | palmitic acid + 2-arachidonoylglycerophosphocholine | - |
? | |
1-stearoyl-2-arachidonoylglycerophosphocholine + H2O | - |
Mesocricetus auratus | stearic acid + 2-arachidonoylglycerophosphocholine | - |
? | |
1-stearoyl-2-arachidonoylglycerophosphoinositol + H2O | - |
Mesocricetus auratus | stearic acid + 2-arachidonoylglycerophosphoinositol | - |
? | |
additional information | the enzyme exhibits both phospholipase A1 and phospholipase A2 activity | Mesocricetus auratus | ? | - |
? | |
additional information | the enzyme is important in phospholipid catabolism and the remodeling of the acyl groups of phospholipids | Mesocricetus auratus | ? | - |
? | |
phosphatidylcholine + H2O | - |
Mesocricetus auratus | ? | - |
? | |
phosphatidylethanolamine + H2O | - |
Mesocricetus auratus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 14000, at least six subunits of the native enzyme can be cross-linked, SDS-PAGE | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mesocricetus auratus |