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Literature summary for 3.1.1.31 extracted from

  • Morales-Luna, L.; Serrano-Posada, H.; Gonzalez-Valdez, A.; Ortega-Cuellar, D.; Vanoye-Carlo, A.; Hernandez-Ochoa, B.; Sierra-Palacios, E.; Rufino-Gonzalez, Y.; Castillo-Rodriguez, R.A.; Perez de la Cruz, V.; Moreno-Vargas, L.; Prada-Gracia, D.; Marcial-Quino, J.; Gomez-Manzo, S.
    Biochemical characterization and structural modeling of fused glucose-6-phosphate dehydrogenase-phosphogluconolactonase from Giardia lamblia (2018), Int. J. Mol. Sci., 19, 2518 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Giardia intestinalis

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure Giardia intestinalis

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride inhibitory at 0.2 M, 40% inhibition at 0.4 M, 65% inhibition at 0.5 M Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 gel filtration Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis A8BWM5 bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase, ECs 1.1.1.49 and 3.1.1.31, respectively
-
Giardia intestinalis ATCC 50803 A8BWM5 bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase, ECs 1.1.1.49 and 3.1.1.31, respectively
-

Purification (Commentary)

Purification (Comment) Organism
the dimer undergoes dissociation at low concentrations, and the monomeric state is catalytically inactive. The enzyme maintains 100% of its activity when it is incubated at a concentration of 50 microg/ml Giardia intestinalis

Subunits

Subunits Comment Organism
homodimer 2 * 83000, calculated from sequence Giardia intestinalis

Synonyms

Synonyms Comment Organism
GL50803_8682 locus name Giardia intestinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42.5
-
 Giardia intestinalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
49.3
-
 50% loss of activity Giardia intestinalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
 melting temperature Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Giardia intestinalis

Expression

Organism Comment Expression
Giardia intestinalis enzyme is active from the log phase on and during the stationary phase additional information