Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Oryctolagus cuniculus | |
diethyl dicarbonate | - |
Oryctolagus cuniculus | |
diisopropyl fluorophosphate | weak | Oryctolagus cuniculus | |
p-bromophenacyl bromide | - |
Oryctolagus cuniculus | |
p-chloromercuriphenyl sulfonic acid | - |
Oryctolagus cuniculus | |
phenylmethylsulfonyl fluoride | weak | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
4-methylumbelliferyl oleate | - |
Oryctolagus cuniculus | |
0.04 | - |
cholesterol oleate | - |
Oryctolagus cuniculus | |
0.05 | - |
triolein | - |
Oryctolagus cuniculus | |
0.07 | - |
diolein | - |
Oryctolagus cuniculus | |
0.2 | - |
monoolein | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Oryctolagus cuniculus | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Oryctolagus cuniculus |
42000 | - |
1 * 42000, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Oryctolagus cuniculus | the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | hydrophobic glycoprotein with variations in its carbohydrate chains | Oryctolagus cuniculus |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
216 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-70°C, in presence of 40% v/v ethylene glycol and 5 mM mercaptoethanol, stable for at least 1 month | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl ester + H2O | with fatty acids of different length in decreasing order, medium chain, long chains, short chains | Oryctolagus cuniculus | 4-methylumbelliferone + fatty acid | - |
? | |
4-methylumbelliferyl oleate + H2O | - |
Oryctolagus cuniculus | 4-methylumbelliferone + oleic acid | - |
? | |
cholesterol oleate + H2O | - |
Oryctolagus cuniculus | cholesterol + oleic acid | - |
? | |
diolein + H2O | - |
Oryctolagus cuniculus | oleic acid + ? | - |
? | |
monoolein + H2O | - |
Oryctolagus cuniculus | oleic acid + glycerol | - |
? | |
additional information | the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes | Oryctolagus cuniculus | ? | - |
? | |
trioleoylglycerol + H2O | - |
Oryctolagus cuniculus | oleic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, 50% inactivation | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.3 | - |
hydrolysis of cholesterol oleate | Oryctolagus cuniculus |
4.5 | - |
hydrolysis of triolein, diolein or monoolein | Oryctolagus cuniculus |
5 | - |
hydrolysis of 4-methylumbelliferyl oleate | Oryctolagus cuniculus |