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Literature summary for 3.1.1.3 extracted from
- Isolation and characterization of an extracellular lipase from the conidia of Neurospora crassa (1987), J. Gen. Microbiol., 133, 149-153.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| trinitrobenzene sulfonic acid | - |
Neurospora crassa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Neurospora crassa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates hydrolysis of triolein | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
2 * 27000, SDS-PAGE | Neurospora crassa |
| 54000 | - |
gel filtration | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme prefers triglycerides with C16-fatty acyl chains and C18-fatty acyl chains, it cleaves only the primary groups of triglycerides, marked preference for substrates containing endogenously occuring fatty acids | Neurospora crassa | ? | - |
? | |
| trilinolein + H2O | - |
Neurospora crassa | linolic acid + ? | - |
? | |
| trioleoylglycerol + H2O | - |
Neurospora crassa | oleic acid + ? | - |
? | |
| tripalmitin + H2O | - |
Neurospora crassa | palmitic acid + ? | - |
? | |
| tristearin + H2O | - |
Neurospora crassa | stearic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 27000, SDS-PAGE | Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Neurospora crassa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | 30°C: optimum, 45°C: 60% of maximal activity | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Neurospora crassa |
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