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Literature summary for 3.1.1.3 extracted from
- Purification and some properties of lipase produced by Pseudomonas fragi 22.39 B (1987), Agric. Biol. Chem., 51, 181-186.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| polyoxyethylene sorbitan monooleate | 0.5%, weak activation | Pseudomonas fragi |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cetyl trimethylammonium bromide | - |
Pseudomonas fragi | |
| Co2+ | 1 mM, 13% inhibition | Pseudomonas fragi | |
| dodecyltrimethylammonium chloride | - |
Pseudomonas fragi | |
| Fe2+ | 1 mM, 63% inhibition | Pseudomonas fragi | |
| Fe3+ | 1 mM, 67% inhibition | Pseudomonas fragi | |
| Hg2+ | 1 mM, 16% inhibition | Pseudomonas fragi | |
| Mn2+ | 1 mM, 18% inhibition | Pseudomonas fragi | |
| n-dodecyltrimethylammonium bromide | - |
Pseudomonas fragi | |
| Pb2+ | 1 mM, 22% inhibition | Pseudomonas fragi | |
| polyoxyethylene oleyl ether | - |
Pseudomonas fragi | |
| Sn2+ | 1 mM, 20% inhibition | Pseudomonas fragi | |
| Sodium dodecyl sulfate | - |
Pseudomonas fragi | |
| sorbitan monooleate | - |
Pseudomonas fragi | |
| Zn2+ | 1 mM, 65% inhibition | Pseudomonas fragi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Pseudomonas fragi |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | inactivates at 45°C | Pseudomonas fragi |
| Methanol | inactivates at 45°C | Pseudomonas fragi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fragi | - |
22-39 B | - |
| Pseudomonas fragi 22-39 B | - |
22-39 B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fragi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas fragi |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Pseudomonas fragi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Pseudomonas fragi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | 40°C: about 55% of maximal activity, 80°C: about 90% of maximal activity | Pseudomonas fragi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
pH 9.0, 24 h, stable up to | Pseudomonas fragi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas fragi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: about 55% of maximal activity, pH 10.0: about 40% of maximal activity | Pseudomonas fragi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.5 | 30°C, 24 h, stable | Pseudomonas fragi |
| 8 | 10.5 | 50°C, 24 h | Pseudomonas fragi |
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