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Literature summary for 3.1.1.3 extracted from

  • Firdaus, M.; Brask, J.; Nielsen, P.; Guo, Z.; Fedosov, S.
    Kinetic model of biodiesel production catalyzed by free liquid lipase from Thermomyces lanuginosus (2016), J. Mol. Catal. B, 133, 55-64 .
No PubMed abstract available

Application

Application Comment Organism
synthesis conversion of plant oil to 92-97% of biodiesel is feasible at 1% enzyme load (24 h, 35°C) using the feedstocks containing 2-20% of water, 0-10% of glycerol, 0-20% of free fatty acids. The enzyme can be collected in a narrow white layer settled between biodiesel and glycerol-water phases,containing also free fatty acids and monoglycerides. The lipase can be then reused after compensation for 5-10% loss of the enzyme. The main contaminants in the produced biodiesel are free fatty acids (2-6%) and monoglycerides (1-3%). Major amounts of free fatty acids and monoglycerides are removed after brief mixing of biodiesel with alkali (2-5% of 5 M NaOH) and centrifugation Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Thermomyces lanuginosus
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