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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of a new inducible thermostable extracellular lipolytic enzyme from the thermophilic archaeon Sulfolobus solfataricus P1 (2016), J. Mol. Catal. B, 124, 11-19.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 5 mM, 51% residual activity | Saccharolobus solfataricus |  |
| diethyl dicarbonate | 0.5 mM, 15% residual activity | Saccharolobus solfataricus | |
| eserine | 5 mM, 8% residual activity | Saccharolobus solfataricus | |
| phenylmethylsulfonyl fluoride | 0.5 mM, 46% residual activity | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0241 | - |
4-nitrophenyl palmitate | pH 6.0, 70°C | Saccharolobus solfataricus | |
| 0.0466 | - |
4-nitrophenyl caprate | pH 6.0, 70°C | Saccharolobus solfataricus | |
| 0.129 | - |
4-nitrophenyl butanoate | pH 6.0, 70°C | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
1 * 45000, SDS-PAGE | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus DSM 1616 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2108 | - |
pH 6.0, 70°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butanoate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl butanoate + H2O | - |
Saccharolobus solfataricus DSM 1616 | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl caprate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl caprate + H2O | - |
Saccharolobus solfataricus DSM 1616 | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + palmitate | best substrate | ? | |
| 4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus DSM 1616 | 4-nitrophenol + palmitate | best substrate | ? | |
| additional information | enzyme shows broad substrate specificity, exhibiting carboxylesterase activity towards short-chain acyl esters and lipase activity toward long-chain acyl esters including triacylglycerols regardless of saturated and unsaturated fatty acids | Saccharolobus solfataricus | ? | - |
? | |
| additional information | enzyme shows broad substrate specificity, exhibiting carboxylesterase activity towards short-chain acyl esters and lipase activity toward long-chain acyl esters including triacylglycerols regardless of saturated and unsaturated fatty acids | Saccharolobus solfataricus DSM 1616 | ? | - |
? | |
| triolein + 3 H2O | - |
Saccharolobus solfataricus | glycerol + 3 oleate | enzyme can hydrolyze all positions of the ester bonds in triolein | ? | |
| triolein + 3 H2O | - |
Saccharolobus solfataricus DSM 1616 | glycerol + 3 oleate | enzyme can hydrolyze all positions of the ester bonds in triolein | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 98 | - |
substrate 4-nitrophenyl palmitate | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to 120 h | Saccharolobus solfataricus |
| 80 | - |
120 h, 50% residual activity | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1135 | - |
4-nitrophenyl butanoate | pH 6.0, 70°C | Saccharolobus solfataricus | |
| 1576 | - |
4-nitrophenyl caprate | pH 6.0, 70°C | Saccharolobus solfataricus | |
| 2252 | - |
4-nitrophenyl palmitate | pH 6.0, 70°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8800 | - |
4-nitrophenyl butanoate | pH 6.0, 70°C | Saccharolobus solfataricus | |
| 33800 | - |
4-nitrophenyl caprate | pH 6.0, 70°C | Saccharolobus solfataricus | |
| 93400 | - |
4-nitrophenyl palmitate | pH 6.0, 70°C | Saccharolobus solfataricus | |
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