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Literature summary for 3.1.1.3 extracted from

  • Li, X.; Yu, H.Y.
    Characterization of an organic solvent-tolerant lipase from Haloarcula sp. G41 and its application for biodiesel production (2014), Folia Microbiol. (Praha), 59, 455-463.
    View publication on PubMed
Search for more literature for 3.1.1.3

Application

Application Comment Organism
biofuel production biodiesel production Haloarcula sp.

General Stability

General Stability Organism
highly stable in the presence of 2.5-25% NaCl Haloarcula sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10 mM, 7.7% inhibition Haloarcula sp.
Cu2+ 1 mM, 19% inhibition Haloarcula sp.
diethyldicarbonate 10 mM, 17% inhibition Haloarcula sp.
EDTA 10 mM, 92% inhibition Haloarcula sp.
Hg2+ 1 mM, 89% inhibition Haloarcula sp.
PMSF 10 mM, 89% inhibition Haloarcula sp.
poly-alpha-olefin 10 mM, 81% inhibition Haloarcula sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Haloarcula sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.3fold stimulation Haloarcula sp.
Mg2+ 1 mM, 1.3fold stimulation Haloarcula sp.
NaCl no activity is detected in the absence of NaCl, optimal activity at 15% Haloarcula sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Haloarcula sp.

Organic Solvent Stability

Organic Solvent Comment Organism
1-decanol 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
Acetone 30% (v/v), half-life: 1 day, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
acetonitrile 30% (v/v), half-life: less than 1 day, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
benzene 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
chloroform 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
cyclohexane 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
dimethylformamide 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
DMSO 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
Ethanol 30% (v/v), half-life: less than 1 day, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
Glycerol 30% (v/v), half-life: 4 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
isooctane 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
Methanol 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
n-hexane 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
tert-Butanol 30% (v/v), half-life: 1 day, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.
toluene 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days Haloarcula sp.

Organism

Organism UniProt Comment Textmining
Haloarcula sp.
-
-
-
Haloarcula sp. G41
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloarcula sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Haloarcula sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O 12% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O 12% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O 20% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O 20% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl decanoate + H2O 85% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl decanoate + H2O 85% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl hexanoate + H2O 30% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O 30% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl laurate + H2O 95% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O maximal activity among the tested 4-nitrophenyl esters Haloarcula sp. 4-nitrophenol + myristate
-
 ?
4-nitrophenyl octanoate + H2O 70% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl octanoate + H2O 70% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl palmitate + H2O 95% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl stearate + H2O 90% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + stearate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Haloarcula sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Haloarcula sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 70% of maximal activity, 80°C: about 70% of maximal activity Haloarcula sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 24 h, stable Haloarcula sp.
90
-
 24 h, 40% loss of activity Haloarcula sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Haloarcula sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 11 pH 6.0: about 55% of maximal activity, pH 11.0: about 50% of maximal activity Haloarcula sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 ighly stable in the pH range of 7.0–11.0 after 24 h incubation Haloarcula sp.