Any feedback?
Literature summary for 3.1.1.3 extracted from
- Biochemical and structural characterization of two site-directed mutants of Staphylococcus xylosus lipase (2010), Mol. Biotechnol., 46, 168-175.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Staphylococcus xylosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V309D | the mutant presents significant increase of lipase activity with unchanged pH and temperature optima in comparison to wild type | Staphylococcus xylosus |
| V309K | the mutant presents significant increase of lipase activity with unchanged thermal stability, pH and temperature optima in comparison to wild type | Staphylococcus xylosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
4-nitrophenyl butyrate | wild type enzyme, at 42°C and pH 9.0 | Staphylococcus xylosus |  |
| 0.64 | - |
4-nitrophenyl butyrate | mutant enzyme V309K, at 42°C and pH 9.0 | Staphylococcus xylosus | |
| 0.76 | - |
4-nitrophenyl butyrate | mutant enzyme V309D, at 42°C and pH 9.0 | Staphylococcus xylosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus xylosus | Q9F0Q9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Staphylococcus xylosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
using 4-nitrophenyl butyrate as substrate, wild type enzyme, at 42°C and pH 9.0 | Staphylococcus xylosus |
| 135.5 | - |
using 4-nitrophenyl butyrate as substrate, mutant enzyme V309D, at 42°C and pH 9.0 | Staphylococcus xylosus |
| 160.4 | - |
using 4-nitrophenyl butyrate as substrate, mutant enzyme V309K, at 42°C and pH 9.0 | Staphylococcus xylosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | - |
Staphylococcus xylosus | 4-nitrophenol + butyrate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Staphylococcus xylosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Staphylococcus xylosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | 35 | the wild type shows 52% activity at 28°C and 63% activity at 35°C | Staphylococcus xylosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
after 30 min of incubation at 80°C the wild type enzyme retains its initial activity | Staphylococcus xylosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.061 | - |
4-nitrophenyl butyrate | wild type enzyme, at 42°C and pH 9.0 | Staphylococcus xylosus | |
| 0.158 | - |
4-nitrophenyl butyrate | mutant enzyme V309K, at 42°C and pH 9.0 | Staphylococcus xylosus | |
| 0.7125 | - |
4-nitrophenyl butyrate | mutant enzyme V309D, at 42°C and pH 9.0 | Staphylococcus xylosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Staphylococcus xylosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | the wild type shows 37% activity at pH 7.0 and 55% activity at pH 5.0 | Staphylococcus xylosus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.141 | - |
4-nitrophenyl butyrate | wild type enzyme, at 42°C and pH 9.0 | Staphylococcus xylosus | |
| 0.164 | - |
4-nitrophenyl butyrate | mutant enzyme V309D, at 42°C and pH 9.0 | Staphylococcus xylosus | |
| 0.247 | - |
4-nitrophenyl butyrate | mutant enzyme V309K, at 42°C and pH 9.0 | Staphylococcus xylosus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
