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Literature summary for 3.1.1.3 extracted from

  • Lan, D.M.; Yang, N.; Wang, W.K.; Shen, Y.F.; Yang, B.; Wang, Y.H.
    A novel cold-active lipase from Candida albicans: cloning, expression and characterization of the recombinant enzyme (2011), Int. J. Mol. Sci., 12, 3950-3965.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes S154L and S154L/S293L are expressed in Pichia pastoris, no obvious expression is observed from wild type and the S293L mutant enzymes Candida albicans

Protein Variants

Protein Variants Comment Organism
S154L the mutation results in loss of enzyme expression Candida albicans
S154L/S293L the mutant exhibits a preference for the short- and medium-chain length 4-nitrophenyl (C4 and C8 acyl group) esters rather than the long chain length 4-nitrophenyl esters (C12, C16 and C18 acyl group), the pure Lip5 double mutant is stable at pH 5.0-9.0 and at temperatures ranging from 15-35°C Candida albicans
S293L the mutation causes a marked reduction in the lipase activity Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 77.22% residual activity at 5 mM Candida albicans
Ca2+ 72.04% residual activity at 5 mM Candida albicans
Cu2+ 78.35% residual activity at 5 mM Candida albicans
EDTA 65.41% residual activity at 5 mM Candida albicans
Fe2+ strong inhibition with residual activity of 20.27% at 5 mM Candida albicans
Fe3+ strong inhibition with residual activity of 35.11% at 5 mM Candida albicans
Hg2+ strong inhibition with residual activity of 13.19% at 5 mM Candida albicans
Li+ 92.24% residual activity at 5 mM Candida albicans
Mg2+ 94.49% residual activity at 1 mM Candida albicans
Mn2+ 90.66% residual activity at 5 mM Candida albicans
Ni2+ 92.24% residual activity at 5 mM Candida albicans
phenylmethylsulfonyl fluoride 38.54% residual activity 5 mM Candida albicans
SDS SDS sharply decreases the lipase activity by 85% at 0.1% (w/v) Candida albicans
Triton X-100 48.55% residual activity at 0.1% (v/v) Candida albicans
Tween 20 82.52% residual activity at 0.1% (v/v) Candida albicans
Tween 80 57.24% residual activity at 0.1% (v/v) Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 4-nitrophenyl caprylate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
0.36
-
 4-nitrophenyl palmitate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
0.41
-
 4-nitrophenyl butyrate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
0.5
-
 4-nitrophenyl laurate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+ increases the recombinant lipase activity at 1 mM to 119.22% or at 5 mM to 115.19% Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, S154L and S154L/S293L mutant enzymes Candida albicans

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone the residual activities of Lip5 double mutant remains at 44.76% after incubation for 1 h in 30% (v/v) acetone Candida albicans
Ethanol the residual activities of Lip5 double mutant remains at 24.32% after incubation for 1 h in 30% (v/v) ethanol Candida albicans
isopropanol lipase activity is almost lost (4.59% residual activity) in the presence of 30% (v/v) isopropanol Candida albicans
Methanol the residual activities of Lip5 double mutant remains at 48.23% after incubation for 1 h in 30% (v/v) methanol Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Candida albicans ATCC 10231
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Q Sepharose column chromatography Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Candida albicans 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Candida albicans ATCC 10231 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caprylate + H2O highest activity of mutant enzyme S154L/S293L Candida albicans 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl caprylate + H2O highest activity of mutant enzyme S154L/S293L Candida albicans ATCC 10231 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl laurate + H2O lowest activity of mutant enzyme S154L/S293L Candida albicans 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O lowest activity of mutant enzyme S154L/S293L Candida albicans ATCC 10231 4-nitrophenol + laurate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Candida albicans 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Candida albicans ATCC 10231 4-nitrophenol + palmitate
-
 ?
additional information the enzyme also uses olive oil and safflower oil as substrates Candida albicans ?
-
 ?
additional information the enzyme also uses olive oil and safflower oil as substrates Candida albicans ATCC 10231 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, S154L and S154L/S293L mutant enzymes Candida albicans

Synonyms

Synonyms Comment Organism
Lip5
-
 Candida albicans
lipase
-
 Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15 25 the recombinant lipase displays the highest activity at 15 and 25°C Candida albicans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 25 the recombinant lipase maintains more than 70% of the maximum activity at 35°C. Even at a lower temperature of 5°C, the recombinant lipase still retains more than 50% of the maximal activity Candida albicans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 45 the Lip5 double mutant shows good thermostability over a range of temperature from 15°C to 45°C, with more than 60% of maximal activity retained after 2 h incubation. However, the stability of recombinant lipase decreases at 55°C, with 23.86% residual activity after incubation for 2 h Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.41
-
 4-nitrophenyl laurate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
31.59
-
 4-nitrophenyl palmitate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
54.18
-
 4-nitrophenyl butyrate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
55.12
-
 4-nitrophenyl caprylate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 the recombinant lipase displays the highest activity at pH 5.0 and 6.0 Candida albicans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the Lip5-double mutant activity exhibits stable within the pH range of 5.0-9.0, and it remains more than 60% of maximal activity. The recombinant lipase sharply loses its activity when incubated at pH 4.0, and only preserves 26% activity after 12 h of incubation Candida albicans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
18.58
-
 4-nitrophenyl laurate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
86.86
-
 4-nitrophenyl palmitate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
131
-
 4-nitrophenyl butyrate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans
203.4
-
 4-nitrophenyl caprylate mutant enzyme S154L/S293L, at pH 6.0 and 25°C Candida albicans