Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Bouaziz, A.; Horchani, H.; Ben Salem, N.; Gargouri, Y.; Sayari, A.
    Expression, purification of a novel alkaline Staphylococcus xylosus lipase acting at high temperature (2011), Biochem. Eng. J., 54, 93-102.
No PubMed abstract available
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus xylosus

Organism

Organism UniProt Comment Textmining
Staphylococcus xylosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity column chromatography Staphylococcus xylosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1500
-
 recombiannt enzyme, at pH 8.5 and 55°C, using olive oil as substrate Staphylococcus xylosus
2850
-
 purified enzyme, at pH 8.5 and 55°C, using olive oil as substrate Staphylococcus xylosus
4100
-
 recombinant enzyme, at pH 8.5 and 55°C, using tributyrin as substrate Staphylococcus xylosus
6300
-
 purified enzyme, at pH 8.5 and 55°C, using tributyrin as substrate Staphylococcus xylosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enyzyme also uses olive oil as substrate Staphylococcus xylosus ?
-
 ?
tributyrin + H2O
-
 Staphylococcus xylosus dibutyrin + butyrate
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Staphylococcus xylosus
SXL2
-
 Staphylococcus xylosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the enzyme remains stable after an incubation of 60 min at 55°C Staphylococcus xylosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 highly stable Staphylococcus xylosus