Literature summary for 3.1.1.3 extracted from

Shangguan, J.J.; Liu, Y.Q.; Wang, F.J.; Zhao, J.; Fan, L.Q.; Li, S.X.; Xu, J.H.
Expression and characterization of a novel lipase from Aspergillus fumigatus with high specific activity (2011), Appl. Biochem. Biotechnol., 165, 949-962.

Search for more literature for 3.1.1.3

Activating Compound

Activating Compound	Comment	Organism	Structure
PEG 20000	124% activity at 0.1% (q/v)	Aspergillus fumigatus
polyvinyl alcohol	118% activity at 0.1% (w/v)	Aspergillus fumigatus
Triton X-100	128% activity at 0.1% (v/v)	Aspergillus fumigatus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Aspergillus fumigatus

Protein Variants

Protein Variants	Comment	Organism
D260A	the mutant shows reduced activity compared to the wild type enzyme	Aspergillus fumigatus
D264A	the mutant shows reduced activity compared to the wild type enzyme	Aspergillus fumigatus
H283A	the mutant shows reduced activity compared to the wild type enzyme	Aspergillus fumigatus
H290A	the mutant shows reduced activity compared to the wild type enzyme	Aspergillus fumigatus
S165A	the mutant shows reduced activity compared to the wild type enzyme	Aspergillus fumigatus

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	47.5% residual activity at 2 mM	Aspergillus fumigatus
Fe2+	57.2% residual activity at 2 mM	Aspergillus fumigatus
Li+	76.4% residual activity at 1 mM	Aspergillus fumigatus
Mg2+	85% residual activity at 2 mM	Aspergillus fumigatus
additional information	not inhibited by Ca2+, Mn2+, Ni2+, Ba2+, and EDTA	Aspergillus fumigatus
SDS	0.201% residual activity at 0.1% (w/v)	Aspergillus fumigatus
Zn2+	32.2% residual activity at 2 mM	Aspergillus fumigatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
14	-	4-nitrophenyl acetate	at 30°C, pH 7.5	Aspergillus fumigatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	metal ions cannot activate the lipase	Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	x * 38000, SDS-PAGE	Aspergillus fumigatus

Organic Solvent Stability

Organic Solvent	Comment	Organism
1-butanol	0.917% residual activity at 50% (v/v), 4.28% residual activity at 10% (v/v)	Aspergillus fumigatus
Acetone	0.917% residual activity at 50% (v/v), 113% activity at 10% (v/v)	Aspergillus fumigatus
DMSO	57.2% residual activity at 50% (v/v), 99.1% activity at 10% (v/v)	Aspergillus fumigatus
Ethanol	2.14% residual activity at 50% (v/v), 100% activity at 10% (v/v)	Aspergillus fumigatus
isopropanol	no activity at 50% (v/v), 83.8% activity at 10% (v/v)	Aspergillus fumigatus
Methanol	0.613% residual activity at 50% (v/v), 108% activity at 10% (v/v)	Aspergillus fumigatus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	-	-	-
Aspergillus fumigatus CGMCC 2873	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Aspergillus fumigatus

Source Tissue

Source Tissue	Comment	Organism	Textmining
mycelium	-	Aspergillus fumigatus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
118	-	supernatant of cell lysate, using 4-nitrophenyl acetate as substrate, at 30°C, pH 7.5	Aspergillus fumigatus
1000	-	after 8.47fold purification, using 4-nitrophenyl acetate as substrate, at 30°C, pH 7.5	Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	100% activity	Aspergillus fumigatus	4-nitrophenol + acetate	-	?
4-nitrophenyl acetate + H2O	100% activity	Aspergillus fumigatus CGMCC 2873	4-nitrophenol + acetate	-	?
4-nitrophenyl butyrate + H2O	about 65% activity compared to 4-nitrophenyl acetate	Aspergillus fumigatus	4-nitrophenol + butyrate	-	?
4-nitrophenyl butyrate + H2O	about 65% activity compared to 4-nitrophenyl acetate	Aspergillus fumigatus CGMCC 2873	4-nitrophenol + butyrate	-	?
4-nitrophenyl decanoate + H2O	about 10% activity compared to 4-nitrophenyl acetate	Aspergillus fumigatus	4-nitrophenol + decanoate	-	?
4-nitrophenyl laurate + H2O	-	Aspergillus fumigatus	4-nitrophenol + laurate	-	?
4-nitrophenyl myristate + H2O	worst substrate, less than 5% activity compared to 4-nitrophenyl acetate	Aspergillus fumigatus	4-nitrophenol + myristate	-	?
4-nitrophenyl propionate + H2O	about 60% activity compared to 4-nitrophenyl acetate	Aspergillus fumigatus	4-nitrophenol + propionate	-	?
4-nitrophenyl propionate + H2O	about 60% activity compared to 4-nitrophenyl acetate	Aspergillus fumigatus CGMCC 2873	4-nitrophenol + propionate	-	?
additional information	AFL1-1 has potential enantioselectivity for the ability to catalyze methyl 3-(4-methoxyphenyl) glycidate and methyl 3-phenylglycidate	Aspergillus fumigatus	?	-	?
additional information	almost no activity on long-chained acyl esters such as 4-nitrophenyl palmitate	Aspergillus fumigatus	?	-	?
additional information	AFL1-1 has potential enantioselectivity for the ability to catalyze methyl 3-(4-methoxyphenyl) glycidate and methyl 3-phenylglycidate	Aspergillus fumigatus CGMCC 2873	?	-	?
additional information	almost no activity on long-chained acyl esters such as 4-nitrophenyl palmitate	Aspergillus fumigatus CGMCC 2873	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 38000, SDS-PAGE	Aspergillus fumigatus

Synonyms

Synonyms	Comment	Organism
AFL1-1	recombinant enzyme	Aspergillus fumigatus
lipase	-	Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	above the optimal temperature, the activity decreases sharply with almost 80% loss of its original activity at 75°C	Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	50	the enzyme is stable (60% activity) at 30-50 °C for 1 h	Aspergillus fumigatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Aspergillus fumigatus

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	10	at pH 7.0, the activity remains at nearly 100% of the maximal activity, and drops dramatically at pH below 9.0	Aspergillus fumigatus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	8.5	the enzyme is stable in the pH range 6.0-8.5 for 16 h (at 4 °C)	Aspergillus fumigatus

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Release 2023.1 (January 2023)