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Literature summary for 3.1.1.3 extracted from

  • Sanchez-Otero, M.G.; Valerio-Alfaro, G.; Garcia-Galindo, H.S.; Oliart-Ros, R.M.
    Immobilization in the presence of Triton X-100: modifications in activity and thermostability of Geobacillus thermoleovorans CCR11 lipase (2008), J. Ind. Microbiol. Biotechnol., 35, 1687-1693.
    View publication on PubMed

General Stability

General Stability Organism
immobilization by adsorption on porous polypropylene (Accurel EP-100) increases enzyme thermostability (shows residual activity even after 1 h at 100°C, when soluble enzyme completely loses activity after 1 h at 80°C) but there is no change in neither the optimum pH nor in pH resistance irrelevant to the presence of Triton X-100 during immobilization Geobacillus thermoleovorans

Organic Solvent Stability

Organic Solvent Comment Organism
SDS activity is completely lost with SDS Geobacillus thermoleovorans
Triton X-100 free enzyme shows less than 50% loss of activity with 0.1% (v/v) Triton X-100, lipase immobilized in the presence of Triton X-100 shows a total loss of activity after 9 cycles of reuse, retaining 53% of its initial activity after seven cycles, while the immobilized lipase without Triton X-100 loses all of its activity after 12 cycles, retaining 69% of its initial activity after 7 cycles Geobacillus thermoleovorans
Tween 80 less than 50% loss of activity with 0.1% (v/v) Tween 80 Geobacillus thermoleovorans

Organism

Organism UniProt Comment Textmining
Geobacillus thermoleovorans Q0JRM8
-
-
Geobacillus thermoleovorans CCR11 Q0JRM8
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by diafiltration Geobacillus thermoleovorans

Storage Stability

Storage Stability Organism
4°C, immobilized enzyme (Accurel EP-100), 36 days, immobilized lipase shows less activity over short chain esters than the soluble lipase Geobacillus thermoleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
Geobacillus thermoleovorans 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Geobacillus thermoleovorans CCR11 4-nitrophenol + laurate
-
?

Synonyms

Synonyms Comment Organism
lipase
-
Geobacillus thermoleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 100 free enzyme retains 100% activity after 1 h at 50°C, immobilized enzyme retains 100% activity after 1 h at 60°C, free enzyme shows 13% residual activity after 1 h at 80°C, immobilized enzyme retains 15.2% residual activity after 1 h at 100°C Geobacillus thermoleovorans