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Literature summary for 3.1.1.3 extracted from
- Identification and characterization of bacterial cutinase (2008), J. Biol. Chem., 283, 25854-25862.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and overexpressed in Escherichia coli cells | Thermobifida fusca |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S170A | mutation of the putative catalytic serine to alanine completely eliminates activity | Thermobifida fusca |
| S188A | mutation of the putative catalytic serine to alanine completely eliminates activity | Thermobifida fusca |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
SDS-PAGE analysis of proteins in the culture supernatant clearly indicates that a polypeptide of 29 kDa is the major protein induced by cutin, suggesting that it might be a candidate for cutinase protein | Thermobifida fusca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida fusca | Q47RJ6 | Tfu_0883 | - |
| Thermobifida fusca | Q47RJ7 | Tfu_0882 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, hydrophobic interaction (phenyl Sepharose), and anion exchange (DEAE-Sepharose) chromatography | Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl butyrate + H2O | - |
Thermobifida fusca | p-nitrophenol + butyrate | - |
? |  |
| triolein + H2O | - |
Thermobifida fusca | 1,2-diolein + oleate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cutinase | - |
Thermobifida fusca |
| pNPB hydrolase | - |
Thermobifida fusca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermobifida fusca |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | temperature dependence of enzyme activity is determined between 20 and 70°C | Thermobifida fusca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | thermostability is evaluated at both 40 and 60°C. Activity increases initially during incubation at both temperatures. It exhibits superior thermostability, with residual activities of over 80% after 160 h at 40°C or over 50% after 40 h at 60°C | Thermobifida fusca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thermobifida fusca |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH dependence of enzyme activity is determined between pH 6.0 and 9.0 | Thermobifida fusca |
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