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Literature summary for 3.1.1.3 extracted from

  • de Pascale, D.; Cusano, A.M.; Autore, F.; Parrilli, E.; di Prisco, G.; Marino, G.; Tutino, M.L.
    The cold-active Lip1 lipase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 is a member of a new bacterial lipolytic enzyme family (2008), Extremophiles, 12, 311-323.
    View publication on PubMed
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Pseudoalteromonas haloplanktis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 43% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
Ca2+ 52% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
Co2+ 36% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
Cu2+ 57% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
EDTA 47% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
Fe3+ complete inhibition at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
Hg2+ 11% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
Mg2+ 54% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
Mn2+ 55% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
phenylmethylsulfonyl fluoride complete inhibition at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
SDS 0.9% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme is associated to the outer membrane Pseudoalteromonas haloplanktis 16020
-

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas haloplanktis Q3IF07
-
-
Pseudoalteromonas haloplanktis TAC 125 Q3IF07
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Q-Sepharose column chromatography Pseudoalteromonas haloplanktis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.079
-
 enzyme from solubilised medium, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis
0.95
-
 after 12fold purification, with 4-nitrophenyl caproate as substrate, at 25°C Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Pseudoalteromonas haloplanktis 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + acetate
-
 ?
4-nitrophenyl caproate + H2O best substrate Pseudoalteromonas haloplanktis 4-nitrophenol + caproate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Pseudoalteromonas haloplanktis 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl octanoate + H2O
-
 Pseudoalteromonas haloplanktis 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl pentanoate + H2O
-
 Pseudoalteromonas haloplanktis 4-nitrophenol + pentanoate
-
 ?
4-nitrophenyl pentanoate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + pentanoate
-
 ?
tricaprylin + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
tricaprylin + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 ?
-
 ?
triolein + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
triolein + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 ?
-
 ?

Synonyms

Synonyms Comment Organism
Lip1
-
 Pseudoalteromonas haloplanktis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 at pH 7.1 Pseudoalteromonas haloplanktis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 50 at pH 7.1 Pseudoalteromonas haloplanktis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 40 activity increases up to 40°C Pseudoalteromonas haloplanktis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 reaches the apparent maximal activity at pH values above 8.5 Pseudoalteromonas haloplanktis