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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of acidic lipase from Aspergillus niger NCIM 1207 (2008), Bioresour. Technol., 100, 1486-1490.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide | complete inhibition at 50 mM | Aspergillus niger |  |
| 2,4,6-Trinitrobenzenesulfonic acid | 55% residual activity at 2 mM | Aspergillus niger | |
| diethyldicarbonate | complete inhibition at 2 mM | Aspergillus niger | |
| additional information | not inhibited by citraconic anhydride (5 mM), N-ethylmaleimide (10 mM), iodoacetate (10 mM), N-acetylimidazole (10 mM), and phenylglyoxal (10 mM) | Aspergillus niger | |
| N-bromosuccinimide | complete inhibition at 0.001 mM | Aspergillus niger | |
| phenylmethylsulfonyl fluoride | 18% residual activity at 5 mM | Aspergillus niger | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
SDS-PAGE | Aspergillus niger |
| 32200 | - |
1 * 32200, MALDI-TOF mass spectrometry | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger NCIM 1207 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephacryl-100 gel filtration | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Aspergillus niger | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Aspergillus niger NCIM 1207 | 4-nitrophenol + palmitate | - |
? | |
| triolein + H2O | - |
Aspergillus niger | 1,2-diolein + oleate | - |
? | |
| triolein + H2O | - |
Aspergillus niger NCIM 1207 | 1,2-diolein + oleate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32200, MALDI-TOF mass spectrometry | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acidic lipase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | the activity decreases sharply above the optimum temperature with almost 95% loss of its original activity at 70°C, the lipase is stable at 40°C for 3 h, incubation at 50°C for 1 h results in 52% loss of lipase activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
- |
Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | isoelectric focusing | - |
8.5 |
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