Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Saisubramanian, N.; Sivasubramanian, S.; Nandakumar, N.; Indirakumar, B.; Chaudhary, N.A.; Puvanakrishnan, R.
    Two step purification of Acinetobacter sp. lipase and its evaluation as a detergent additive at low temperatures (2008), Appl. Biochem. Biotechnol., 150, 139-156.
    View publication on PubMed
Search for more literature for 3.1.1.3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 SDS-PAGE Acinetobacter sp.

Organic Solvent Stability

Organic Solvent Comment Organism
hydrogen peroxide the enzyme shows stability in the presence of 30% (w/v) hydrogen peroxide Acinetobacter sp.
additional information slight decrease in activity in the presence of commercial detergents Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp. MTCC 6816
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified by by polyethylene glycol-salt aqueous two phase system and octyl Sepharose column chromatography Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.9
-
 crude extract, in 0.1 M Tris buffer, pH 8.5 Acinetobacter sp.
88.8
-
 after 14.92fold purification, in 0.1 M Tris buffer, pH 8.5 Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
 Acinetobacter sp. 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O
-
 Acinetobacter sp. MTCC 6816 4-nitrophenol + laurate
-
 ?
olive oil + H2O
-
 Acinetobacter sp. ?
-
 ?
olive oil + H2O
-
 Acinetobacter sp. MTCC 6816 ?
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Acinetobacter sp.
triacylglycerol hydrolase
-
 Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 50 the lipase is active at low temperatures and retains 86.8% activity at 10°C, when the enzyme is incubated with 0.1 M Tris buffer, pH 8.5 for 1 h, it is stable up to 50°C Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Acinetobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9
-
 Acinetobacter sp.