Any feedback?
Literature summary for 3.1.1.3 extracted from
- Purification and characterization of an extracellular lipase from Clostridium tetanomorphum (2006), World J. Microbiol. Biotechnol., 22, 431-435.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | growth medium containing glycerol in addition to fermentable substrates, such as L-glutamate or glucose, induces the extracellular enzyme | Clostridium tetanomorphum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Clostridium tetanomorphum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
native PAGE | Clostridium tetanomorphum |
| 118000 | - |
gel filtration and sedimentation coefficient | Clostridium tetanomorphum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium tetanomorphum | - |
- |
- |
| Clostridium tetanomorphum NCTC 543 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 27fold by ammonium sulfate fractionation and anion exchange chromatography, and ultrafiltration to homogeneity | Clostridium tetanomorphum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | growth medium containing glycerol in addition to fermentable substrates, such as L-glutamate or glucose, induces the extracellular enzyme | Clostridium tetanomorphum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 900 | - |
purified extracellular lipase | Clostridium tetanomorphum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | with p-nitrophenyl esters of different fatty acids as substrates enzyme activity is highest when the acyl chain is short e.g. C2 | Clostridium tetanomorphum | ? | - |
? |  |
| additional information | with p-nitrophenyl esters of different fatty acids as substrates enzyme activity is highest when the acyl chain is short e.g. C2 | Clostridium tetanomorphum NCTC 543 | ? | - |
? | |
| tributyrin + H2O | - |
Clostridium tetanomorphum | dibutyrin + butyrate | - |
? | |
| tributyrin + H2O | - |
Clostridium tetanomorphum NCTC 543 | dibutyrin + butyrate | - |
? | |
| tricaprin + H2O | - |
Clostridium tetanomorphum | dicaprin + caprate | - |
? | |
| tricaproin + H2O | - |
Clostridium tetanomorphum | dicaproin + caproate | - |
? | |
| tricaprylin + H2O | - |
Clostridium tetanomorphum | dicaprylin + caprylate | - |
? | |
| trilaurin + H2O | - |
Clostridium tetanomorphum | dilaurin + laurate | - |
? | |
| trilaurin + H2O | - |
Clostridium tetanomorphum NCTC 543 | dilaurin + laurate | - |
? | |
| triolein + H2O | - |
Clostridium tetanomorphum | diolein + oleate | - |
? | |
| triolein + H2O | - |
Clostridium tetanomorphum NCTC 543 | diolein + oleate | - |
? | |
| tripalmitin + H2O | - |
Clostridium tetanomorphum | dipalmitin + palmitate | - |
? | |
| tripalmitin + H2O | - |
Clostridium tetanomorphum NCTC 543 | dipalmitin + palmitate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| extracellular lipase | - |
Clostridium tetanomorphum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
