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Literature summary for 3.1.1.3 extracted from
- Expression, purification, and characterization of His-tagged Staphylococcus xylosus lipase wild-type and its mutant Asp 290 Ala (2006), Protein Expr. Purif., 47, 516-523.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | - |
Staphylococcus xylosus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) | Staphylococcus xylosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D290A | site-directed mutagenesis, the mutant shows reduced activity with tributyrin substrate compared to the wild-type enzyme, the mutation does not influence calcium requirement | Staphylococcus xylosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Staphylococcus xylosus | |
| EGTA | - |
Staphylococcus xylosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, recombinant His-tagged wild-type and mutant enzymes | Staphylococcus xylosus | |
| 20.4 | - |
tributyrin | pH 8.5, 37°C, recombinant His-tagged wild-type enzyme | Staphylococcus xylosus | |
| 31.25 | - |
tributyrin | pH 8.5, 37°C, native wild-type enzyme | Staphylococcus xylosus | |
| 34.5 | - |
tributyrin | pH 8.5, 37°C, recombinant His-tagged mutant D290A | Staphylococcus xylosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Staphylococcus xylosus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates, the enzyme is not dependent on Ca2+ | Staphylococcus xylosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Staphylococcus xylosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus xylosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes 18fold and 8.8fold, respectively, from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography to homogeneity | Staphylococcus xylosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 850 | - |
purified recombinant His-tagged wild-type enzyme, substrate olive oil | Staphylococcus xylosus |
| 1000 | - |
purified recombinant His-tagged mutant D290A, substrate tributyrin or olive oil | Staphylococcus xylosus |
| 1500 | - |
purified recombinant His-tagged wild-type enzyme, substrate tributyrin | Staphylococcus xylosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | residue Asp290 is important for the chain length specificity and catalytic efficiency of the enzyme | Staphylococcus xylosus | ? | - |
? | |
| olive oil + H2O | - |
Staphylococcus xylosus | ? | - |
? | |
| tributyrin + H2O | - |
Staphylococcus xylosus | dibutyrin + butyrate | - |
? | |
| trioctanoate + H2O | - |
Staphylococcus xylosus | dioctanoate + octanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Staphylococcus xylosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Staphylococcus xylosus |
| SXL | - |
Staphylococcus xylosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Staphylococcus xylosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1200 | - |
tributyrin | pH 8.5, 37°C, recombinant His-tagged mutant D290A | Staphylococcus xylosus | |
| 1500 | - |
tributyrin | pH 8.5, 37°C, recombinant His-tagged wild-type enzyme | Staphylococcus xylosus | |
| 1900 | - |
tributyrin | pH 8.5, 37°C, native wild-type enzyme | Staphylococcus xylosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Staphylococcus xylosus |
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