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Literature summary for 3.1.1.3 extracted from

  • Song, H.T.; Jiang, Z.B.; Ma, L.X.
    Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216 (2006), Protein Expr. Purif., 47, 393-397.
    View publication on PubMed
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
gene lip7, DNA and amino acid sequence determination and anaylsis, expression of His6-tagged LipY7p in Pichia pastoris strain KM71, expression pattern, overview Yarrowia lipolytica
gene lip8, DNA and amino acid sequence determination and anaylsis, expression of His6-tagged LipY8p in Pichia pastoris strain KM71, expression pattern, overview Yarrowia lipolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, recombinant LipY7p, SDS-PAGE Yarrowia lipolytica
41000
-
 x * 41000, recombinant LipY8p, SDS-PAGE Yarrowia lipolytica

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO at 50%, 3 h, room temperature, purified recombinant LipY7p, over 60% remaining activity Yarrowia lipolytica
DMSO at 50%, 3 h, room temperature, purified recombinant LipY8p, over 60% remaining activity Yarrowia lipolytica

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica Q872L3 LIPY8
-
Yarrowia lipolytica Q872L4 LipY7p
-
Yarrowia lipolytica AS 2.1216 Q872L3 LIPY8
-
Yarrowia lipolytica AS 2.1216 Q872L4 LipY7p
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant LipY7p expressed in Pichia pastoris is slightly glycosylated Yarrowia lipolytica
glycoprotein the recombinant LipY8p expressed in Pichia pastoris is slightly glycosylated Yarrowia lipolytica

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged LipY7p from Pichia pastoris strain KM71 by nickel affinity chromatography Yarrowia lipolytica
recombinant His6-tagged LipY8p from Pichia pastoris strain KM71 by nickel affinity chromatography Yarrowia lipolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57.8
-
 purified recombinant LipY8p Yarrowia lipolytica
62.6
-
 purified recombinant LipY7p Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
 Yarrowia lipolytica 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O
-
 Yarrowia lipolytica AS 2.1216 4-nitrophenol + laurate
-
 ?
additional information substrate specificity of LipY8p, overview Yarrowia lipolytica ?
-
 ?
additional information substrate specificity of LipY8p, overview Yarrowia lipolytica AS 2.1216 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, recombinant LipY7p, SDS-PAGE Yarrowia lipolytica
? x * 41000, recombinant LipY8p, SDS-PAGE Yarrowia lipolytica

Synonyms

Synonyms Comment Organism
lipase
-
 Yarrowia lipolytica
LipY7p
-
 Yarrowia lipolytica
LipY8p
-
 Yarrowia lipolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Yarrowia lipolytica
45
-
-
 Yarrowia lipolytica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 3 h, purified recombinant LipY7p, pH 7.0, 85% remaining activity Yarrowia lipolytica
65
-
 3 h, purified recombinant LipY8p, pH 7.0, 85% remaining activity Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Yarrowia lipolytica

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 3 h, room temperature, recombinant LipY7p, 75% remaining activity Yarrowia lipolytica
5 10 3 h, room temperature, recombinant LipY8p, 75% remaining activity Yarrowia lipolytica