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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of the extracellular lipase Lip2 from Yarrowia lipolytica (2007), Process Biochem., 42, 384-391.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the LIP2 is not affected by EDTA, SDS, Tween 80, Span 65, Span 85, Triton X-100, DTT, and 2-mercaptoethanol | Yarrowia lipolytica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lip2, DNA and amino acid sequence determination and analysis | Yarrowia lipolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Yarrowia lipolytica |  |
| Fe2+ | slight inhibition | Yarrowia lipolytica | |
| K+ | slight inhibition | Yarrowia lipolytica | |
| additional information | the LIP2 is not affected by Mg2+, Mn2+, EDTA, SDS, Tween 80, Span 65, Span 85, Triton X-100, DTT, and 2-mercaptoethanol | Yarrowia lipolytica | |
| Ni2+ | - |
Yarrowia lipolytica | |
| Zn2+ | - |
Yarrowia lipolytica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Yarrowia lipolytica | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates 1.23fold at 5 mM | Yarrowia lipolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, LIP2, SDS-PAGE | Yarrowia lipolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| olive oil + H2O | Yarrowia lipolytica | - |
? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 30°C, 30 min, about 90% remaining activity at 10%, inactivation at 20% | Yarrowia lipolytica |
| DMSO | 30°C, 30 min, about 90% remaining activity at 10%, 95% activity at 20% | Yarrowia lipolytica |
| Ethanol | 30°C, 30 min, about 90% remaining activity at 10%, inactivation at 20% | Yarrowia lipolytica |
| isopropanol | 30°C, 30 min, about 90% remaining activity at 10%, inactivation at 20% | Yarrowia lipolytica |
| Methanol | 30°C, 30 min, about 90% remaining activity at 10%, 60% activity at 20% | Yarrowia lipolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | Q9P8F7 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | LIP2 is a glycosylated protein which contains about 12% sugar, the deglycosylated lipase retains 88% specific activity compared to the untreated lipase | Yarrowia lipolytica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 26.5fold by solvent precipitation, anion exchange and hydrophobic interaction chromatography | Yarrowia lipolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21300 | - |
purified enzyme, substrate olive oil | Yarrowia lipolytica |
| 22900 | - |
purified enzyme, substrate safflower oil | Yarrowia lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| flax seed oil + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| methyl myristate + H2O | best fatty acid methyl ester substrate | Yarrowia lipolytica | methanol + myristate | - |
? | |
| additional information | the lipase showed a preference for long chain fatty acid methyl esters of C12-C16, substrate specificity, overview | Yarrowia lipolytica | ? | - |
? | |
| olive oil + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| safflower oil + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| soybean oil + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, LIP2, SDS-PAGE | Yarrowia lipolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| extracellular lipase | - |
Yarrowia lipolytica |
| LIP2 | - |
Yarrowia lipolytica |
| YlLip2 | - |
Yarrowia lipolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Yarrowia lipolytica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | sharp drop of activity above 45°C, inactive at 60°C | Yarrowia lipolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | 4 h, 30°C and 35°C, the purified lipase retains 95% and 83% activity, respectively, 32% and 5% remaining activity after 4 h at 40°C and 45°C, respectively | Yarrowia lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Yarrowia lipolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | activity range, the activity drops off rapidly at pH above 8.0 with only about 20% of maximal activity remaining at pH 9.0 | Yarrowia lipolytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | purified enzyme, 3 h, 25°C, stable | Yarrowia lipolytica |
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