Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CHAPS | - |
Psychrobacter sp. | |
additional information | effects of detergents on LipA1 activity, overview | Psychrobacter sp. | |
SDS | - |
Psychrobacter sp. | |
Span 40 | - |
Psychrobacter sp. | |
Span 60 | - |
Psychrobacter sp. | |
Triton X-100 | - |
Psychrobacter sp. | |
Tween 40 | - |
Psychrobacter sp. | |
Tween 80 | - |
Psychrobacter sp. |
Cloned (Comment) | Organism |
---|---|
gene lipA1, DNA and amino acid sequence determination and analysis, screening a genomic DNA library, expression of LipA1 in Escherichia coli strain BL21 (DE3) | Psychrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Psychrobacter sp. | |
Co2+ | - |
Psychrobacter sp. | |
EDTA | - |
Psychrobacter sp. | |
Fe2+ | - |
Psychrobacter sp. | |
Fe3+ | - |
Psychrobacter sp. | |
Hg2+ | - |
Psychrobacter sp. | |
additional information | effects of detergents on LipA1 activity, overview | Psychrobacter sp. | |
Rb+ | - |
Psychrobacter sp. | |
Zn2+ | - |
Psychrobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Psychrobacter sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Psychrobacter sp. | |
Mg2+ | activates | Psychrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35210, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE | Psychrobacter sp. |
35210 | - |
x * 35210, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE | Psychrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychrobacter sp. | Q2KTB5 | psychrotrophic strain 7195 isolated from deepsea sediment of Prydz Bay, gene lipA1 | - |
Purification (Comment) | Organism |
---|---|
recombinant LipA1 35fold from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, gel filtration, and preparative polyacrylamide gel electrophoresis | Psychrobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | LipA1 is a cold-adapted enzyme and has one consensus motif GNSMG, containing the putative active-site serine, which is conserved in other cold-adapted lipolytic enzymes | Psychrobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | optimal and highest temperatures for growth of strain 7195 are 15°C and 35°C, respectively, exhibiting sigmoidal growth even at -3°C, no growth at 37°C or above | Psychrobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1084 | - |
purified recombinant enzyme | Psychrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprate + H2O | high activity | Psychrobacter sp. | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl myristate + H2O | best 4-nitrophenyl ester substrate | Psychrobacter sp. | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | high activity | Psychrobacter sp. | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + stearate | - |
? | |
additional information | substrate specificity, LipA1 shows a preference for trimyristin and 4-nitrophenyl myristate, overview | Psychrobacter sp. | ? | - |
? | |
olive oil + H2O | high activity | Psychrobacter sp. | ? | - |
? | |
triacetin + H2O | - |
Psychrobacter sp. | diacetin + acetate | - |
? | |
tributyrin + H2O | - |
Psychrobacter sp. | dibutyrin + butyrate | - |
? | |
tricaprin + H2O | - |
Psychrobacter sp. | dicaprin + caprate | - |
? | |
trimyristin + H2O | best triacylglyceride substrate | Psychrobacter sp. | dimyristin + myristate | - |
? | |
triolein + H2O | - |
Psychrobacter sp. | diolein + oleate | - |
? | |
tripalmitin + H2O | high activity | Psychrobacter sp. | dipalmitin + palmitate | - |
? | |
tristearin + H2O | - |
Psychrobacter sp. | distearin + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35210, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE | Psychrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
LipA1 | - |
Psychrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Psychrobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | purified enzyme, substrate 4-nitrophenyl palmitate, maximal activity at 30°C, 18% and 35% of maximal activity at 0°C and 10°C, respectively | Psychrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
purified LipA1, completely stable up to | Psychrobacter sp. |
30 | - |
purified LipA1, 5 h, 50% remaining activity | Psychrobacter sp. |
60 | - |
purified LipA1, 15 min 50% remaining activity | Psychrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Psychrobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | purified enzyme, 24 h, 0°C, 90% reamining activity | Psychrobacter sp. |