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Literature summary for 3.1.1.3 extracted from
- Crab digestive lipase acting at high temperature: purification and biochemical characterization (2007), Biochimie, 89, 1012-1018.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | the enzyme is inhibited by bile salts | Homarus americanus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Homarus americanus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homarus americanus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 165fold from hepatopancreas by heat treatment, ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and a second gel filtration step | Homarus americanus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive gland | - |
Homarus americanus | - |
| hepatopancreas | - |
Homarus americanus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 500 | - |
purified native enzyme | Homarus americanus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| olive oil + H2O | - |
Homarus americanus | ? | - |
? | |
| tributyrin + H2O | - |
Homarus americanus | dibutyrin + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Homarus americanus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDL | - |
Homarus americanus |
| crab digestive lipase | - |
Homarus americanus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Homarus americanus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 60 | 10% of maximal activity at 37°C, maximal activity at 60°C, complete inactivation at 65°C | Homarus americanus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homarus americanus |
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