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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of two highly thermophilic alkaline lipases from Thermosyntropha lipolytica (2007), Appl. Environ. Microbiol., 73, 7725-7731.
General Stability
| General Stability | Organism |
|---|---|
| 2 mM ammonium sulfate, bovine serum albumin at 2 mg/ml, or 40% v/v glycerol stabilize the cold-labile purified enzyme | Thermosyntropha lipolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the enzyme activity is not affected by Ca2+ at 1-10 mM, 15% inhibition at 25 mM at pH 9.4-9.6 | Thermosyntropha lipolytica |  |
| EDTA | complete inhibition at 10 mM | Thermosyntropha lipolytica | |
| PMSF | complete inhibition at 10 mM | Thermosyntropha lipolytica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | LipA and LipB | Thermosyntropha lipolytica | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | both LipA and LipB contain one ion per monomeric enzyme unit, the enzyme activity is not affected by Ca2+ at 1-10 mM, 15% inhibition at 25 mM at pH 9.4-9.6 | Thermosyntropha lipolytica | |
| Mn2+ | both LipA and LipB contain one ion per monomeric enzyme unit, the addition of 1 mM MnCl2 to dialyzed enzyme preparations enhances the activities at 96°C of both LipA and LipB by 3fold and increases the durations of their thermal stability at 60°C and 75°C, respectively, by 4 h | Thermosyntropha lipolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, LipA, SDS-PAGE, x * 57000, LipB, SDS-PAGE | Thermosyntropha lipolytica |
| 57000 | - |
x * 50000, LipA, SDS-PAGE, x * 57000, LipB, SDS-PAGE | Thermosyntropha lipolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermosyntropha lipolytica | - |
strain DSM 11003, an anaerobic, thermophilic, alkali-tolerant bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native LipA and LipB 140fold and 134fold, respectively, from cell culture supernatant by ammonium sulfate fractionation amd hydrophobic interaction chromatography to homogeneity | Thermosyntropha lipolytica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | optimal growth at pH 7.6 and 60°C, overview | Thermosyntropha lipolytica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.4 | - |
purified LipB | Thermosyntropha lipolytica |
| 14 | - |
purified LipA | Thermosyntropha lipolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, purified LipA and LipB, irreversible loss of activity of both isozymes, after 24 h or when frozen in various buffers, including phosphate buffer, pH 8.0, determined at 25°C, bovine serum albumin at 2 mg/ml or 40% v/v glycerol stabilize the purified enzyme | Thermosyntropha lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl laurate + H2O | - |
Thermosyntropha lipolytica | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Thermosyntropha lipolytica | 4-nitrophenol + palmitate | - |
? | |
| additional information | LipA and LipB exhibit high activity with long-chain fatty acid glycerides, yielding maximum activity with trioleate and, among the 4-nitrophenyl esters, with 4-nitrophenyl laurate, hydrolysis of glycerol ester bonds occurs at positions 1 and 3, tributyrin and tricaproin are poor substrates, overview | Thermosyntropha lipolytica | ? | - |
? | |
| triolein + H2O | - |
Thermosyntropha lipolytica | diolein + oleate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, LipA, SDS-PAGE, x * 57000, LipB, SDS-PAGE | Thermosyntropha lipolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LipA | - |
Thermosyntropha lipolytica |
| LipB | - |
Thermosyntropha lipolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 96 | - |
about, LipA and LipB | Thermosyntropha lipolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
addition of 1 mM MnCl2 to dialyzed enzyme preparations increases the durations of their thermal stability at 60°C and 75°C, respectively, by 4 h | Thermosyntropha lipolytica |
| 100 | - |
LipA and LipB, at pH 8.0, determined at 25°C, retain 50% activity after 6 h and 2 h of incubation in presence of 2 M ammonium sulfate, respectively | Thermosyntropha lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at, pH is determined at 25°C | Thermosyntropha lipolytica |
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