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Literature summary for 3.1.1.3 extracted from

  • Li, H.; Zhang, X.
    Characterization of thermostable lipase from thermophilic Geobacillus sp. TW1 (2005), Protein Expr. Purif., 42, 153-159.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ recombinant enzyme Geobacillus stearothermophilus
Li+ recombinant enzyme Geobacillus stearothermophilus
Mn2+ recombinant enzyme Geobacillus stearothermophilus
additional information no inhibition by EDTA, PMSF, 2-mercaptoethanol, PMSF, DTT, Tween 20, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonic acid, or Triton X-100 Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, recombinant enzyme Geobacillus stearothermophilus
Fe2+ activates, recombinant enzyme Geobacillus stearothermophilus
Fe3+ activates, recombinant enzyme Geobacillus stearothermophilus
Mg2+ activates, recombinant enzyme Geobacillus stearothermophilus
Zn2+ activates, recombinant enzyme Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q3T5M2 i.e. Bacillus stearothermophilus, strain TW1, isolated from a hot spring in China, gene lip2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
LIP2
-
Geobacillus stearothermophilus
lipase
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
optimal growth temperature is 65°C Geobacillus stearothermophilus
40
-
recombinant enzyme Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
stable up to, purified recombinant enzyme Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 recombinant enzyme Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 stable, purified recombinant enzyme Geobacillus stearothermophilus