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Literature summary for 3.1.1.3 extracted from

  • Chang, S.W.; Lee, G.C.; Shaw, J.F.
    Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase (2006), J. Agric. Food Chem., 54, 815-822.
    View publication on PubMed
Search for more literature for 3.1.1.3

Application

Application Comment Organism
additional information codon optimization for improved expression of LIP1 in Pichia pastoris for uuse of the enzyme in industrial processes Diutina rugosa

Cloned(Commentary)

Cloned (Comment) Organism
gene lip1, expression in Pichia pastoris, culture condition and codon optimization for improved expression, secretion of the recombinant enzyme, overview Diutina rugosa

Protein Variants

Protein Variants Comment Organism
additional information construction of a regional synthetic lip1 gene for improved expression in Pichia pastoris, overview Diutina rugosa

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by Tween 80, Brij35, Triton X-100, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonic acid, and sodium taurocholate at 0.1-1.0% w/v Diutina rugosa
SDS 98% inhibition at 0.1% w/v Diutina rugosa
Tween 20 18-19% inhibition at 0.1-1.0% w/v Diutina rugosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, recombinant enzyme, SDS-PAGE Diutina rugosa

Organism

Organism UniProt Comment Textmining
Diutina rugosa P20261 LIP1 precrusor; i.e. Candida cylindracea, gene lip1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant secreted enzyme 25.6fold from Pichia pastoris by hydrophobic interaction chromatography Diutina rugosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
 recombinant enzyme in culture medium Diutina rugosa
7200
-
 purified recombinant enzyme, substrate 4-nitrophenyl laurate Diutina rugosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Diutina rugosa 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caproate + H2O good substrate Diutina rugosa 4-nitrophenol + caproate
-
 ?
4-nitrophenyl caprylate + H2O
-
 Diutina rugosa 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl decanoate + H2O good substrate Diutina rugosa 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl laurate + H2O best substrate Diutina rugosa 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O
-
 Diutina rugosa 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O lower activity Diutina rugosa 4-nitrophenol + palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, recombinant enzyme, SDS-PAGE Diutina rugosa

Synonyms

Synonyms Comment Organism
Lip1
-
 Diutina rugosa
lipase
-
 Diutina rugosa
More the enzyme belongs to the CRL multigne family Diutina rugosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Diutina rugosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 10 min, recombinant enzyme, pH 7.0, stable at, inactivation above Diutina rugosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant enzyme Diutina rugosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 14 h, 95-100% remaining activity Diutina rugosa