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Literature summary for 3.1.1.3 extracted from
- Topological characterization and modeling of the 3D structure of lipase from Pseudomonas aeruginosa (1993), FEBS Lett., 332, 143-149.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Pseudomonas aeruginosa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, SDS-PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa PAC1R | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | protease V8 cleaves between surface residues Asp38 and Glu46 | Pseudomonas aeruginosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad is formed by Ser82, Asp229, and His251 | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| triacetin + H2O | - |
Pseudomonas aeruginosa | diacetin + acetate | - |
? | |
| triacetin + H2O | - |
Pseudomonas aeruginosa PAC1R | diacetin + acetate | - |
? | |
| tripropionin + H2O | - |
Pseudomonas aeruginosa | dipropionin + propionate | - |
? | |
| tripropionin + H2O | - |
Pseudomonas aeruginosa PAC1R | dipropionin + propionate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Pseudomonas aeruginosa |
| More | the enzyme contains a single disulfide bond formed by Cys183 and Cys235 hidden inside the enzyme molecule, three-dimensional structure determination, analysis, and modelling | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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