General Stability | Organism |
---|---|
lyophilization of the crude enzyme leads to only slight loss of activity | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% inhibition at 2.5 mM | Pseudomonas aeruginosa | |
EDTA | 60% inhibition at 1 mM, completely reversible by 5 mM MgSO4 | Pseudomonas aeruginosa | |
eserine | 27% inhibition at 5 mM | Pseudomonas aeruginosa | |
additional information | no inhibition of extracellular enzyme by 1 M NaCl, 0.1 mg/ml heparin, and SH-reagents 4-hydroymercuribenzoate, 2-mercaptoethanol, iodoacetate, sodium diphosphate, and NaF | Pseudomonas aeruginosa | |
protamine | 50% inhibition at 2 mg/ml | Pseudomonas aeruginosa | |
sodium taurocholate | 67% inhibition at 5 mM | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the extracellular enzyme form is secreted to the culture medium | Pseudomonas aeruginosa | - |
- |
intracellular | second, minor enzyme form | Pseudomonas aeruginosa | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
strain 10145, ATCC | - |
Pseudomonas aeruginosa ATCC 10145 | - |
strain 10145, ATCC | - |
Purification (Comment) | Organism |
---|---|
extracellular enzyme 35fold from culture medium by gel filtration and disc electrophoresis | Pseudomonas aeruginosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | extracellular enzyme expression during growth phases, profile | Pseudomonas aeruginosa | - |
Storage Stability | Organism |
---|---|
-20°C, crude enzyme, stable for at least one month with repeated freezing and thawing | Pseudomonas aeruginosa |
22°C, room temperature, the crude enzyme is stable for a few hours | Pseudomonas aeruginosa |
4°C, overnight, loss of 60% activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acylglycerol + H2O | - |
Pseudomonas aeruginosa | glycerol + a carboxylate | - |
? | |
acylglycerol + H2O | - |
Pseudomonas aeruginosa ATCC 10145 | glycerol + a carboxylate | - |
? | |
diacylglycerol + H2O | - |
Pseudomonas aeruginosa | acylglycerol + a carboxylate | - |
? | |
diacylglycerol + H2O | - |
Pseudomonas aeruginosa ATCC 10145 | acylglycerol + a carboxylate | - |
? | |
additional information | the enzyme is unspecific, but prefers substrates with shorter side chains, exo-enzyme | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme is unspecific, but prefers substrates with shorter side chains, exo-enzyme | Pseudomonas aeruginosa ATCC 10145 | ? | - |
? | |
triacylglycerol + H2O | - |
Pseudomonas aeruginosa | diacylglycerol + a carboxylate | - |
? | |
triacylglycerol + H2O | - |
Pseudomonas aeruginosa ATCC 10145 | diacylglycerol + a carboxylate | - |
? | |
tributyrin + H2O | - |
Pseudomonas aeruginosa | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Pseudomonas aeruginosa ATCC 10145 | dibutyrin + butyrate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol ester hydrolase | - |
Pseudomonas aeruginosa |
lipase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
extracellular enzyme | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
50 min, 50% remaining activity | Pseudomonas aeruginosa |
60 | - |
3 min, 50% remaining activity | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
extracellular enzyme | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | - |
Pseudomonas aeruginosa |