Cloned (Comment) | Organism |
---|---|
amino acid determinatioin of the purified enzyme, high-level expression in Pichia pastoris GS115, subcloning in Escherichia coli DH5alpha | Geobacillus thermocatenulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propanol | - |
Geobacillus thermocatenulatus | |
acetone | - |
Geobacillus thermocatenulatus | |
cholate | 1% w/v, 20% decrease in activity | Geobacillus thermocatenulatus | |
methanol | - |
Geobacillus thermocatenulatus | |
SDS | 1% w/v, 95% decrease in activity | Geobacillus thermocatenulatus | |
Triton X-100 | 1% w/v, 30% decrease in activity | Geobacillus thermocatenulatus | |
Tween 20 | 1% w/v, 7% decrease in activity | Geobacillus thermocatenulatus | |
Tween 80 | 1% w/v, 6% decrease in activity | Geobacillus thermocatenulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, recombinant enzyme, SDS-PAGE | Geobacillus thermocatenulatus |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 47% reduced activity | Geobacillus thermocatenulatus |
Acetone | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 16% reduced activity | Geobacillus thermocatenulatus |
Methanol | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 16% reduced activity | Geobacillus thermocatenulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermocatenulatus | - |
- |
- |
Geobacillus thermocatenulatus BTL2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
large scale from Pichia pastoris culture supernatant, 660fold | Geobacillus thermocatenulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23000 | - |
purified enzyme, substrate tributyrin | Geobacillus thermocatenulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Geobacillus thermocatenulatus | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Geobacillus thermocatenulatus BTL2 | 4-nitrophenol + palmitate | - |
? | |
triacetin + H2O | poor substrate | Geobacillus thermocatenulatus | diacetin + acetate | - |
? | |
tributyrin + H2O | best substrate | Geobacillus thermocatenulatus | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | best substrate | Geobacillus thermocatenulatus BTL2 | dibutyrin + butyrate | - |
? | |
tricaprin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dicaprin + decaprate | - |
? | |
tricaproin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dicaproin + caproate | - |
? | |
trilaurin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | dilaurin + laurate | - |
? | |
trilaurin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus BTL2 | dilaurin + laurate | - |
? | |
trimyristin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | dimyristin + myristate | - |
? | |
triolein + H2O | - |
Geobacillus thermocatenulatus | diolein + oleate | - |
? | |
triolein + H2O | - |
Geobacillus thermocatenulatus BTL2 | diolein + oleate | - |
? | |
tripalmitin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dipalmitin + palmitate | - |
? | |
tripalmitin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus BTL2 | dipalmitin + palmitate | - |
? | |
tristearin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | distearin + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, recombinant enzyme, SDS-PAGE | Geobacillus thermocatenulatus |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Geobacillus thermocatenulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
with substrate tributyrin, pH 8.0 | Geobacillus thermocatenulatus |
75 | - |
with substrate triolein, pH 8.0 | Geobacillus thermocatenulatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 80 | - |
Geobacillus thermocatenulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to, pH 8.0, 0.1 M Tris buffer | Geobacillus thermocatenulatus |
70 | - |
18% remaining activity | Geobacillus thermocatenulatus |
80 | - |
5% remaining activity | Geobacillus thermocatenulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
with substrate tributyrin, 60°C | Geobacillus thermocatenulatus |
9 | - |
with substrate triolein, 60°C | Geobacillus thermocatenulatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Geobacillus thermocatenulatus |