Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Saxena, R.K.; Davidson, W.S.; Sheoran, A.; Giri, B.
    Purification and characterization of an alkaline thermostable lipase from Aspergillus carneus (2003), Process Biochem., 39, 239-247.
No PubMed abstract available
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
hexadecyl trimethyl ammonium bromide strong activation Aspergillus carneus
additional information no effect by metal chelators Aspergillus carneus
n-octyl-alpha-D-glucopyranoside strong activation Aspergillus carneus
n-octyl-beta-D-glucopyranoside strong activation Aspergillus carneus
taurocholic acid strong activation, 2.7fold at 10 mM Aspergillus carneus

General Stability

General Stability Organism
half-life of the enzyme is enhanced by glycine, sorbitol, glycerol, glucose, and ammonium chloride Aspergillus carneus

Inhibitors

Inhibitors Comment Organism Structure
Bromosuccinimide
-
 Aspergillus carneus
cholic acid
-
 Aspergillus carneus
diethyl 4-nitrophenyl phosphate strong inhibition Aspergillus carneus
additional information no effect by metal chelators, no inhibition by trinitrobenzene sulfonic acid, N-ethylmaleimide, p-chloromercuribenzoate, o-phenanthroline, and 4,4'-dipyridyl-disulfide Aspergillus carneus
N-lauroyl sarcosine strong inhibition Aspergillus carneus
PMSF strong inhibition Aspergillus carneus
SDS strong inhibition Aspergillus carneus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme follows first order reaction kinetics during inactivation Aspergillus carneus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ acivation Aspergillus carneus
Na+ acivation Aspergillus carneus
NH4+ acivation Aspergillus carneus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 about, native PAGE Aspergillus carneus

Organic Solvent Stability

Organic Solvent Comment Organism
Butanol 80% loss of activity after 30 min, complete loss of activity after 24 h Aspergillus carneus
Ethanol complete loss of activity after 30 min in 90% ethanol, loss of 41% activity after 30 min in 40% ethanol Aspergillus carneus
additional information the enzyme is highly active in presence of many different organic solvents, overview Aspergillus carneus
propan-2-ol 97% loss of activity after 30 min in 60% propan-2-ol, loss of only 3% activity after 30 min in 30% propan-2-ol Aspergillus carneus

Organism

Organism UniProt Comment Textmining
Aspergillus carneus
-
 alkaline thermostable lipase
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 12.3% glycosylated Aspergillus carneus

Purification (Commentary)

Purification (Comment) Organism
24.1fold Aspergillus carneus

Reaction

Reaction Comment Organism Reaction ID
triacylglycerol + H2O = diacylglycerol + a carboxylate a tryptophan residue plays an important role in maintaining the ative conformation of the enzyme Aspergillus carneus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
502
-
 purified enzyme Aspergillus carneus

Storage Stability

Storage Stability Organism
22°C, room temperature, lyophilized enzyme, completely stable for more than 1 year Aspergillus carneus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O low activity Aspergillus carneus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl caproate + H2O
-
 Aspergillus carneus 4-nitrophenol + caproate
-
 ?
4-nitrophenyl laurate + H2O enzyme shows a preference for lauric acid and a 1,3-position specificity Aspergillus carneus 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O
-
 Aspergillus carneus 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Aspergillus carneus 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl stearate + H2O low activity Aspergillus carneus 4-nitrophenol + stearate
-
 ?
additional information the enzyme utilizes olive oil as a substrate, no activity with 2-nitrophenyl palmitate Aspergillus carneus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Aspergillus carneus

Synonyms

Synonyms Comment Organism
lipase
-
 Aspergillus carneus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Aspergillus carneus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 90
-
 Aspergillus carneus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermostability is enhanced by glycine, sorbitol, glycerol, glucose, and ammonium chloride Aspergillus carneus
70
-
 5 min, stable, after 10 min enzyme shows loss of 45% activity Aspergillus carneus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aspergillus carneus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus carneus isoelectric focusing 4.4 4.3