Application | Comment | Organism |
---|---|---|
synthesis | useful in application to the processing of industrial fats and oils containing eicosapentanoic acid and docosahexaenoic acid, such as fish oil splitting | Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.12fold activation at 1 mM, 1.36fold activation at 3 mM, 1.51fold activation at 10 mM | Pseudomonas fluorescens | |
K+ | slight activation | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
gel filtration | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens HU380 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
24.3fold | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9854 | - |
purified enzyme | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl butyrate + H2O | - |
Pseudomonas fluorescens | methanol + butyrate | - |
? | |
methyl butyrate + H2O | - |
Pseudomonas fluorescens HU380 | methanol + butyrate | - |
? | |
methyl caprate + H2O | best substrate | Pseudomonas fluorescens | methanol + caprate | - |
? | |
methyl caproate + H2O | - |
Pseudomonas fluorescens | methanol + caproate | - |
? | |
methyl caprylate + H2O | - |
Pseudomonas fluorescens | methanol + caprylate | - |
? | |
methyl icosanoate + H2O | - |
Pseudomonas fluorescens | methanol + icosanoate | - |
? | |
methyl laurate + H2O | - |
Pseudomonas fluorescens | methanol + laurate | - |
? | |
methyl linoleate + H2O | - |
Pseudomonas fluorescens | methanol + linoleate | - |
? | |
methyl linolenate + H2O | - |
Pseudomonas fluorescens | methanol + linolenate | - |
? | |
methyl myristate + H2O | - |
Pseudomonas fluorescens | methanol + myristate | - |
? | |
methyl oleate + H2O | - |
Pseudomonas fluorescens | methanol + oleate | - |
? | |
methyl palmitate + H2O | - |
Pseudomonas fluorescens | methanol + palmitate | - |
? | |
methyl propionate + H2O | - |
Pseudomonas fluorescens | methanol + propionate | - |
? | |
methyl propionate + H2O | - |
Pseudomonas fluorescens HU380 | methanol + propionate | - |
? | |
methyl stearate + H2O | - |
Pseudomonas fluorescens | methanol + stearate | - |
? | |
additional information | substrate specificity overview, enzyme splits polyunsaturated fatty acid ester bonds, the enzyme preferably acts on short- to middle-chain length fatty acid simple methyl esters and triglycerides | Pseudomonas fluorescens | ? | - |
? | |
additional information | substrate specificity overview, enzyme splits polyunsaturated fatty acid ester bonds, the enzyme preferably acts on short- to middle-chain length fatty acid simple methyl esters and triglycerides | Pseudomonas fluorescens HU380 | ? | - |
? | |
olive oil + H2O | emulsion | Pseudomonas fluorescens | ? | - |
? | |
triacetin + H2O | low activity | Pseudomonas fluorescens | diacetin + acetate | - |
? | |
tributyrin + H2O | best substrate | Pseudomonas fluorescens | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | best substrate | Pseudomonas fluorescens HU380 | dibutyrin + butyrate | - |
? | |
tricaprin + H2O | best substrate | Pseudomonas fluorescens | dicaprin + caprate | - |
? | |
tricaproin + H2O | best substrate | Pseudomonas fluorescens | dicaproin + caproate | - |
? | |
tricaprylin + H2O | best substrate | Pseudomonas fluorescens | dicaprylin + caprylate | - |
? | |
trilaurin + H2O | - |
Pseudomonas fluorescens | dilaurin + laurate | - |
? | |
trimyristin + H2O | low activity | Pseudomonas fluorescens | dimyristin + myristate | - |
? | |
triolein + H2O | cleaves mainly 1,3-ester bonds, and to a lesser extent the 2-position ester bond | Pseudomonas fluorescens | diolein + oleate | - |
? | |
triolein + H2O | cleaves mainly 1,3-ester bonds, and to a lesser extent the 2-position ester bond | Pseudomonas fluorescens HU380 | diolein + oleate | - |
? | |
tripalmitin + H2O | low activity | Pseudomonas fluorescens | dipalmitin + palmitate | - |
? | |
tristearin + H2O | - |
Pseudomonas fluorescens | distearin + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
70°C, stable for 60 min, in presence of 0.1% Triton X-100 | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas fluorescens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas fluorescens | isoelectric focusing | 4.8 | 4.7 |