BRENDA - Enzyme Database
show all sequences of 3.1.1.3

Production, purification, characterization, and applications of lipases

Sharma, R.; Chisti, Y.; Banerjee, U.C.; Biotechnol. Adv. 19, 627-662 (2001) View publication on PubMed

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
castor oil
at 2% as carbon source, enhances the enzyme production in vivo, pH 6.9
Pseudomonas aeruginosa
additional information
enzyme is not affected by benzamidine and PMSF
Rhizopus oryzae
additional information
the lipase of strain F-111 is unaffected by various detergents
Pseudomonas alcaligenes
Triton X-100
activation, strain F-111
Pseudomonas aeruginosa
Triton X-100
enhances the enzyme production in vivo by 50fold compared to olive oil alone in the medium
Rhizomucor miehei
Tween 80
activation, strain F-111
Pseudomonas aeruginosa
yeast extract
increases growth and enzyme production rate at 50°C, strain -12
Bacillus sp. (in: Bacteria)
Application
Application
Commentary
Organism
detergent
strain M1, enzyme is used for removal of fatty stains under conditions of a modern machine wash and in alkaline environment
Pseudomonas alcaligenes
paper production
removal of pitch, i.e. triglycerides and waxes, from the pulp produced for paper making
Diutina rugosa
synthesis
enantioselective hydrolysis of chiral esters
Pseudomonas aeruginosa
synthesis
immobilized enzyme is used for the transesterification reaction that replaces pamitic acid in palm oil with stearic acid
Rhizomucor miehei
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Geobacillus stearothermophilus
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Aspergillus niger
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Pseudomonas sp.
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Acinetobacter calcoaceticus
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Proteus vulgaris
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Rhodotorula glutinis
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Staphylococcus epidermidis
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Rhizopus oryzae
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Bacillus sp. (in: Bacteria)
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Burkholderia cepacia
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Aspergillus oryzae
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Penicillium roqueforti
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Diutina rugosa
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Moesziomyces antarcticus
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Hyphopichia burtonii
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Burkholderia sp.
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Penicillium wortmanii
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Saccharomyces cerevisiae, mature enzyme encoded by gene geh SE1 is overexpressed in Escherichia coli as N-terminally HIs-tagged enzyme
Staphylococcus epidermidis
gene tglA, enzyme form L3, DNA and amino acid sequence determination and analysis, functional expressionin Escherichia coli, Aspergillus oryzae is used as a host for expression of Thermomyces lanuginosus
Aspergillus oryzae
isozyme gene lip1, synthetically synthesized in an optimized nucleotide sequence to avoid the interference that occurs in heterologous expression of the native gene due to unusual codon usage, functional overexpression in Pichia pastoris of the synthetic gene, expression in Candida maltosa, a related yeast with the same codon usage, secretion in active form to the culture medium
Diutina rugosa
strain DSM 853, cloning and epitope mapping
Rhizopus oryzae
strain IGB83, cloning and functional expression in Xanthomonas campestris, secretion of the recombinant enzyme to the medium, expression of the enzyme also in Escherichia coli as His-tagged protein, secretion to the medium
Pseudomonas aeruginosa
strain M1, DNA sequencing and analysis, for functional expression in Escherichia coli the coexpression of a lipase-specific foldase, encoded by lipH, is required to ensure the correct folding and secretion of the recombinant enzyme through the host membrane
Pseudomonas alcaligenes
Engineering
Protein Variants
Commentary
Organism
additional information
enzyme can be immobilized on Amberlite IRC50 with good long-term stability and high adsorption capacity
Rhizopus oryzae
additional information
immobilization of lipase B on Sepharose, alumina, silica, for the latter using precursors Si(OCH3)3 or Si(OCH3)4, providing a highly active, chemically and thermally stable, heterogeneous biocatalyst
Moesziomyces antarcticus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
-
Penicillium roqueforti
Cd2+
strain KKA-5, slight inhibition
Pseudomonas aeruginosa
Cu2+
strain KKA-5, slight inhibition
Pseudomonas aeruginosa
Cu2+
strong inhibition
Rhizopus oryzae
Fe2+
-
Penicillium roqueforti
Fe2+
strain KKA-5, strong inhibition
Pseudomonas aeruginosa
Fe2+
strong inhibition
Rhizopus oryzae
Fe3+
strain KKA-5, strong inhibition
Pseudomonas aeruginosa
Fe3+
60% inhibition at 1 mM
Pseudomonas oleovorans
Fe3+
strong inhibition
Rhizopus oryzae
Hg2+
-
Penicillium roqueforti
Hg2+
strain KKA-5, strong inhibition
Pseudomonas aeruginosa
Hg2+
strong inhibition
Rhizopus oryzae
isopropyl fluorophosphate
-
Penicillium roqueforti
Mn2+
strain KKA-5, slight inhibition
Pseudomonas aeruginosa
additional information
enzyme from strain A30-1 is stable to alkaline protease treatment
Bacillus sp. (in: Bacteria)
additional information
no inhibition by Ca2+, Mg2+, Mn2+, Na+, K+, Cu2+, EDTA, p-chloromercuribenzoate, and iodoacetate
Penicillium roqueforti
additional information
strain KKA-5, no inhibition by Ca2+ and Mg2+
Pseudomonas aeruginosa
additional information
the lipase of strain F-111 is unaffected by various detergents
Pseudomonas alcaligenes
additional information
no inhibition by Ca2+, Hg2+, Zn2+, Mn2+, Cu2+, Mg2+, Co2+, Cd2+, Pb2+, EDTA, and o-phenanthrolin
Pseudomonas oleovorans
additional information
enzyme is not affected by benzamidine and PMSF
Rhizopus oryzae
oleic acid
inhibits enzyme production, product inhibition
Diutina rugosa
SDS
strain F-111
Pseudomonas aeruginosa
Zn2+
strain KKA-5, strong inhibition, strain MB5001: 94% inhibition at 1 mM
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
4-nitrophenyl laurate
-
Rhodotorula glutinis
2.7
-
4-nitrophenyl butyrate
-
Rhodotorula glutinis
12
-
4-nitrophenyl palmitate
-
Burkholderia cepacia
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Pseudomonas sp.
-
-
extracellular
-
Proteus vulgaris
-
-
extracellular
-
Pseudomonas oleovorans
-
-
extracellular
especially in a lypolytic strain
Rhizopus oryzae
-
-
extracellular
L1, L2, and L3
Aspergillus oryzae
-
-
extracellular
strain F-111
Pseudomonas alcaligenes
-
-
extracellular
strain KKA-5, strain MB5001, strain IGB83
Pseudomonas aeruginosa
-
-
intracellular
-
Rhizopus oryzae
5622
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
activation, extracellular enzyme
Bacillus sp. (in: Bacteria)
Ca2+
required
Acinetobacter calcoaceticus
Ca2+
activation, extracellular enzyme
Bacillus sp. (in: Bacteria)
Ca2+
increases enzyme expression
Bacillus sp. (in: Bacteria)
Ca2+
native enzyme, required for activity
Staphylococcus epidermidis
CaCl2
1.24fold stimulation at 1 mM
Pseudomonas aeruginosa
Co2+
required
Acinetobacter calcoaceticus
Cu2+
required
Acinetobacter calcoaceticus
Iron
increases enzyme expression
Bacillus sp. (in: Bacteria)
Mg2+
required
Acinetobacter calcoaceticus
Mg2+
enhances enzyme expression
Aspergillus niger
Mg2+
increases enzyme expression
Bacillus sp. (in: Bacteria)
Mg2+
required for strain F-111 and KKA-5, maximal enzyme production of strain KKA-5 at 0.8 M Mg2+
Pseudomonas oleovorans
additional information
Ca2+-concentration does not affect the enzyme production
Pseudomonas oleovorans
additional information
strain A30-1 requires a complexed medium which must contains diverse metal ions, overview
Bacillus sp. (in: Bacteria)
Na+
activation, extracellular enzyme
Bacillus sp. (in: Bacteria)
taurocholic acid
1.6fold stimulation at 200 mM
Pseudomonas aeruginosa
Tween 80
increases growth and enzyme production rate at 50°C, strain -12
Bacillus sp. (in: Bacteria)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
enzyme form L1
Aspergillus oryzae
25000
-
x * 25000, SDS-PAGE
Penicillium roqueforti
29000
-
x * 29000, enzyme of strain MB5001, SDS-PAGE, x * 32000, enzyme from strain F-111, SDS-PAGE, x * 30000, enzyme of strain KKA-5, SDS-PAGE
Pseudomonas aeruginosa
30000
-
x * 29000, enzyme of strain MB5001, SDS-PAGE, x * 32000, enzyme from strain F-111, SDS-PAGE, x * 30000, enzyme of strain KKA-5, SDS-PAGE
Pseudomonas aeruginosa
30000
-
x * 33000, enzyme from strain KM1-56, SDS-PAGE, x * 30000, extracellular enzyme, SDS-PAGE
Pseudomonas sp.
31000
-
1 * 31000, SDS-PAGE
Proteus vulgaris
32000
-
extracellular enzyme, gel filtration
Rhizopus oryzae
32000
-
x * 29000, enzyme of strain MB5001, SDS-PAGE, x * 32000, enzyme from strain F-111, SDS-PAGE, x * 30000, enzyme of strain KKA-5, SDS-PAGE
Pseudomonas aeruginosa
32000
-
x * 32000, strain BD 413, SDS-PAGE
Acinetobacter calcoaceticus
33000
-
x * 33000, enzyme from strain KM1-56, SDS-PAGE, x * 30000, extracellular enzyme, SDS-PAGE
Pseudomonas sp.
35000
-
1 * 35000 + 1 * 46000, recombinant enzyme, SDS-PAGE
Staphylococcus epidermidis
45000
-
1 * 45000, strain J33
Bacillus sp. (in: Bacteria)
46000
-
1 * 35000 + 1 * 46000, recombinant enzyme, SDS-PAGE
Staphylococcus epidermidis
51000
-
gel filtration
Hyphopichia burtonii
80000
90000
recombinant enzyme, gel filtration and native PAGE
Staphylococcus epidermidis
Organic Solvent Stability
Organic Solvent
Commentary
Organism
1,1,1-trichloroethane
enzyme catalyzes the regioselective hydrolysis of preacetylated monosaccharide derivatives in
Aspergillus niger
hexane
estrification of lactic acid and alcohols in hexane
Moesziomyces antarcticus
n-heptane
enzyme performs the hydrolysis of 4-nitrophenyl palmitate in n-heptane
Burkholderia cepacia
n-heptane
the purified enzyme is less active in dry n-heptane than the crude preparation, but the addition of a small amount of water dramatically activates the purified enzyme but not the crude one
Diutina rugosa
toluene
catalyzes the esterification of sulcatol and fatty acids in toluene
Diutina rugosa
Organism
Organism
UniProt
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Acinetobacter calcoaceticus BD 413
-
-
-
Aspergillus niger
-
-
-
Aspergillus oryzae
-
2 enzyme forms L1, L2, and L3
-
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) J33
-
-
-
Burkholderia cepacia
-
-
-
Burkholderia sp.
-
-
-
Diutina rugosa
-
dimorphic yeast, several lipase isoforms encoded by the lip1 gene family
-
Geobacillus stearothermophilus
-
L1
-
Geobacillus stearothermophilus L1
-
L1
-
Hyphopichia burtonii
-
-
-
Moesziomyces antarcticus
-
multiple enzyme forms
-
Penicillium roqueforti
-
-
-
Penicillium roqueforti IAM7268
-
-
-
Penicillium wortmanii
-
-
-
Proteus vulgaris
-
highly alkaline enzyme
-
Pseudomonas aeruginosa
-
strain KKA-5, strain MB5001
-
Pseudomonas alcaligenes
-
strain M1, strain F-111
-
Pseudomonas oleovorans
-
strain F-111, strain KKA-5
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. KM1-56
-
-
-
Rhizomucor miehei
-
-
-
Rhizopus oryzae
-
lipolytic strain
-
Rhodotorula glutinis
-
-
-
Staphylococcus epidermidis
-
strain RP 62A, strain 9
-
Oxidation Stability
Oxidation Stability
Organism
enzyme from strain A30-1 is stable to H2O2
Bacillus sp. (in: Bacteria)
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
strain 9 contains an enzyme which is organized as a preproenzyme
Staphylococcus epidermidis
Purification (Commentary)
Purification (Commentary)
Organism
-
Proteus vulgaris
enzyme form L1, to homogeneity
Aspergillus oryzae
extracellular enzyme, 1200fold
Rhizopus oryzae
from strain KW1-56: 13.9fold, extracellular enzyme: 37fold to homogeneity
Pseudomonas sp.
from strain MB5001 in a 3-step procedure, from strain F-111 to homogeneity, 518fold from strain KKA-5 to homogeneity
Pseudomonas aeruginosa
native enzyme to homogeneity, recombinant enzyme rROL from Saccharomyces cerevisiae, recombinant His-tagged mature enzyme
Staphylococcus epidermidis
partial from strain A30-1, 175fold from strain J33
Bacillus sp. (in: Bacteria)
strain BD413, to homogeneity
Acinetobacter calcoaceticus
to homogeneity
Penicillium roqueforti
to homogeneity
Hyphopichia burtonii
Reaction
Reaction
Commentary
Organism
Reaction ID
triacylglycerol + H2O = diacylglycerol + a carboxylate
enzyme contains the conserved pentapeptide Ala-Xaa-Ser-Xaa-Gly
Bacillus sp. (in: Bacteria)
triacylglycerol + H2O = diacylglycerol + a carboxylate
enzyme form L3: catalytic triad is formed by conserved Ser, His and Asp residues
Aspergillus oryzae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
batch or fed-batch culture in a medium containing citric acid and soybean oil as substrates
Pseudomonas alcaligenes
-
cell culture
enzyme production in presence of 1% olive oil or corn oil in the culture medium
Bacillus sp. (in: Bacteria)
-
cell culture
submerged batch culture
Diutina rugosa
-
additional information
culture conditions, the extracellular enzyme is dependent on a special nitrogen source in the medium, e.g. peptone, while the intracellular enzyme form is not
Rhizopus oryzae
-
additional information
high enzyme production
Penicillium wortmanii
-
additional information
medium conditions for the cell culture
Pseudomonas aeruginosa
-
additional information
medium conditions for the cell culture
Pseudomonas oleovorans
-
additional information
strain A30-1 requires a complexed medium which must contains diverse metal ions, overview
Bacillus sp. (in: Bacteria)
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-nitrophenyl butyrate + H2O
-
650654
Rhodotorula glutinis
4-nitrophenol + butyrate
-
650654
Rhodotorula glutinis
?
4-nitrophenyl caprylate + H2O
L1, best substrate
650654
Geobacillus stearothermophilus
4-nitrophenol + caprylate
-
650654
Geobacillus stearothermophilus
?
4-nitrophenyl caprylate + H2O
preferred substrate of lipase A
650654
Diutina rugosa
4-nitrophenol + caprylate
-
650654
Diutina rugosa
?
4-nitrophenyl caprylate + H2O
L1, best substrate
650654
Geobacillus stearothermophilus L1
4-nitrophenol + caprylate
-
650654
Geobacillus stearothermophilus L1
?
4-nitrophenyl laurate + H2O
-
650654
Rhodotorula glutinis
4-nitrophenol + laurate
-
650654
Rhodotorula glutinis
?
4-nitrophenyl laurate + H2O
preferred substrate of lipase B
650654
Diutina rugosa
4-nitrophenol + laurate
-
650654
Diutina rugosa
?
4-nitrophenyl laurate + H2O
purified enzyme of strain F-111 shows preference for
650654
Pseudomonas aeruginosa
4-nitrophenol + laurate
-
650654
Pseudomonas aeruginosa
?
4-nitrophenyl myristate + H2O
purified enzyme of strain F-111 shows preference for
650654
Pseudomonas aeruginosa
4-nitrophenol + myristate
-
650654
Pseudomonas aeruginosa
?
4-nitrophenyl palmitate + H2O
-
650654
Acinetobacter calcoaceticus
4-nitrophenol + palmitate
-
650654
Acinetobacter calcoaceticus
?
4-nitrophenyl palmitate + H2O
in n-heptane
650654
Burkholderia cepacia
4-nitrophenol + palmitate
-
650654
Burkholderia cepacia
?
4-nitrophenyl palmitate + H2O
-
650654
Acinetobacter calcoaceticus BD 413
4-nitrophenol + palmitate
-
650654
Acinetobacter calcoaceticus BD 413
?
castor oil + H2O
-
650654
Pseudomonas aeruginosa
?
-
650654
Pseudomonas aeruginosa
?
methyl beta-D-glucopyranoside + H2O
regioselective deacetylation of preacylated substrate
650654
Aspergillus niger
methanol + beta-D-glucose
-
650654
Aspergillus niger
?
additional information
enantioselective transesterification of 2-phenoxy-1-propanol
650654
Pseudomonas sp.
?
-
650654
Pseudomonas sp.
?
additional information
highly specific for short-chain fatty acids esters
650654
Penicillium roqueforti
?
-
650654
Penicillium roqueforti
?
additional information
immobilized enzyme performs the transesterification reaction that replaces pamitic acid in palm oil with stearic acid
650654
Rhizomucor miehei
?
-
650654
Rhizomucor miehei
?
additional information
lipases A and B display no interfacial activation due to absence of the lid structure which regulates the access to the active site. The enzyme performs estrification of lactic acid and alcohols in hexane
650654
Moesziomyces antarcticus
?
-
650654
Moesziomyces antarcticus
?
additional information
strain A30-1, active with triglycerides of C16:0 to C22:0fatty acids and on natural fats and oils
650654
Bacillus sp. (in: Bacteria)
?
-
650654
Bacillus sp. (in: Bacteria)
?
additional information
substrates are long acyl chain 4-nitrophenol esters
650654
Acinetobacter calcoaceticus
?
-
650654
Acinetobacter calcoaceticus
?
additional information
highly specific for short-chain fatty acids esters
650654
Penicillium roqueforti IAM7268
?
-
650654
Penicillium roqueforti IAM7268
?
additional information
strain A30-1, active with triglycerides of C16:0 to C22:0fatty acids and on natural fats and oils
650654
Bacillus sp. (in: Bacteria) J33
?
-
650654
Bacillus sp. (in: Bacteria) J33
?
additional information
substrates are long acyl chain 4-nitrophenol esters
650654
Acinetobacter calcoaceticus BD 413
?
-
650654
Acinetobacter calcoaceticus BD 413
?
additional information
enantioselective transesterification of 2-phenoxy-1-propanol
650654
Pseudomonas sp. KM1-56
?
-
650654
Pseudomonas sp. KM1-56
?
R-ketoprofen + H2O
lipase B is stereoselective for the R-isomer in an antichiral solvent such as isopentyl methyl ketone or S(+)-carvone
650654
Moesziomyces antarcticus
?
-
650654
Moesziomyces antarcticus
?
rac 2-(4-chlorophenoxy)propanoic acid + n-butanol
esterification reaction, in n-heptane
650654
Diutina rugosa
?
-
650654
Diutina rugosa
?
sulcatol + fatty acid
esterification reaction, in toluene
650654
Diutina rugosa
?
-
650654
Diutina rugosa
?
tert-butyl octanoate + H2O
preferred substrate, high activity with
650654
Burkholderia sp.
tert-butanol + octanoate
-
650654
Burkholderia sp.
?
tributyrin + H2O
-
650654
Geobacillus stearothermophilus
dibutyrin + butyrate
-
650654
Geobacillus stearothermophilus
?
tributyrin + H2O
-
650654
Aspergillus niger
dibutyrin + butyrate
-
650654
Aspergillus niger
?
tributyrin + H2O
-
650654
Pseudomonas aeruginosa
dibutyrin + butyrate
-
650654
Pseudomonas aeruginosa
?
tributyrin + H2O
-
650654
Pseudomonas sp.
dibutyrin + butyrate
-
650654
Pseudomonas sp.
?
tributyrin + H2O
-
650654
Acinetobacter calcoaceticus
dibutyrin + butyrate
-
650654
Acinetobacter calcoaceticus
?
tributyrin + H2O
-
650654
Proteus vulgaris
dibutyrin + butyrate
-
650654
Proteus vulgaris
?
tributyrin + H2O
-
650654
Rhodotorula glutinis
dibutyrin + butyrate
-
650654
Rhodotorula glutinis
?
tributyrin + H2O
-
650654
Staphylococcus epidermidis
dibutyrin + butyrate
-
650654
Staphylococcus epidermidis
?
tributyrin + H2O
-
650654
Rhizopus oryzae
dibutyrin + butyrate
-
650654
Rhizopus oryzae
?
tributyrin + H2O
-
650654
Bacillus sp. (in: Bacteria)
dibutyrin + butyrate
-
650654
Bacillus sp. (in: Bacteria)
?
tributyrin + H2O
-
650654
Pseudomonas alcaligenes
dibutyrin + butyrate
-
650654
Pseudomonas alcaligenes
?
tributyrin + H2O
-
650654
Burkholderia cepacia
dibutyrin + butyrate
-
650654
Burkholderia cepacia
?
tributyrin + H2O
-
650654
Aspergillus oryzae
dibutyrin + butyrate
-
650654
Aspergillus oryzae
?
tributyrin + H2O
-
650654
Penicillium roqueforti
dibutyrin + butyrate
-
650654
Penicillium roqueforti
?
tributyrin + H2O
-
650654
Diutina rugosa
dibutyrin + butyrate
-
650654
Diutina rugosa
?
tributyrin + H2O
-
650654
Pseudomonas oleovorans
dibutyrin + butyrate
-
650654
Pseudomonas oleovorans
?
tributyrin + H2O
-
650654
Rhizomucor miehei
dibutyrin + butyrate
-
650654
Rhizomucor miehei
?
tributyrin + H2O
-
650654
Moesziomyces antarcticus
dibutyrin + butyrate
-
650654
Moesziomyces antarcticus
?
tributyrin + H2O
-
650654
Hyphopichia burtonii
dibutyrin + butyrate
-
650654
Hyphopichia burtonii
?
tributyrin + H2O
-
650654
Burkholderia sp.
dibutyrin + butyrate
-
650654
Burkholderia sp.
?
tributyrin + H2O
-
650654
Penicillium wortmanii
dibutyrin + butyrate
-
650654
Penicillium wortmanii
?
tributyrin + H2O
-
650654
Penicillium roqueforti IAM7268
dibutyrin + butyrate
-
650654
Penicillium roqueforti IAM7268
?
tributyrin + H2O
-
650654
Bacillus sp. (in: Bacteria) J33
dibutyrin + butyrate
-
650654
Bacillus sp. (in: Bacteria) J33
?
tributyrin + H2O
-
650654
Acinetobacter calcoaceticus BD 413
dibutyrin + butyrate
-
650654
Acinetobacter calcoaceticus BD 413
?
tributyrin + H2O
-
650654
Geobacillus stearothermophilus L1
dibutyrin + butyrate
-
650654
Geobacillus stearothermophilus L1
?
tributyrin + H2O
-
650654
Pseudomonas sp. KM1-56
dibutyrin + butyrate
-
650654
Pseudomonas sp. KM1-56
?
triolein + H2O
hydrolysis at all positions, strain J33
650654
Bacillus sp. (in: Bacteria)
diolein + oleate
-
650654
Bacillus sp. (in: Bacteria)
?
triolein + H2O
preferred substrate of enzyme form L1
650654
Aspergillus oryzae
diolein + oleate
-
650654
Aspergillus oryzae
?
triolein + H2O
hydrolysis at all positions, strain J33
650654
Bacillus sp. (in: Bacteria) J33
diolein + oleate
-
650654
Bacillus sp. (in: Bacteria) J33
?
Subunits
Subunits
Commentary
Organism
?
x * 25000, SDS-PAGE
Penicillium roqueforti
?
1 * 31000, SDS-PAGE
Proteus vulgaris
?
x * 29000, enzyme of strain MB5001, SDS-PAGE, x * 32000, enzyme from strain F-111, SDS-PAGE, x * 30000, enzyme of strain KKA-5, SDS-PAGE
Pseudomonas aeruginosa
?
x * 32000, strain BD 413, SDS-PAGE
Acinetobacter calcoaceticus
?
x * 33000, enzyme from strain KM1-56, SDS-PAGE, x * 30000, extracellular enzyme, SDS-PAGE
Pseudomonas sp.
dimer
1 * 35000 + 1 * 46000, recombinant enzyme, SDS-PAGE
Staphylococcus epidermidis
monomer
1 * 51000, SDS-PAGE
Hyphopichia burtonii
monomer
1 * 24000, enzyme form L1
Aspergillus oryzae
monomer
1 * 32000, extracellular enzyme, SDS-PAGE
Rhizopus oryzae
monomer
1 * 45000, strain J33
Bacillus sp. (in: Bacteria)
Synonyms
Synonyms
Commentary
Organism
amano AP
-
Moesziomyces antarcticus
amano B
-
Moesziomyces antarcticus
amano CE
-
Moesziomyces antarcticus
amano CES
-
Moesziomyces antarcticus
amano P
-
Moesziomyces antarcticus
amno N-AP
-
Moesziomyces antarcticus
BAL
-
Moesziomyces antarcticus
Bile-salt-stimulated lipase
-
Moesziomyces antarcticus
BSSL
-
Moesziomyces antarcticus
butyrinase
-
Moesziomyces antarcticus
cacordase
-
Moesziomyces antarcticus
CALB
-
Moesziomyces antarcticus
capalase L
-
Moesziomyces antarcticus
Carboxyl ester lipase
-
Moesziomyces antarcticus
cholesterol esterase
-
Moesziomyces antarcticus
CRL
-
Diutina rugosa
Cytotoxic T lymphocyte lipase
-
Moesziomyces antarcticus
EDL
-
Moesziomyces antarcticus
endothelial cell-derived lipase
-
Moesziomyces antarcticus
endothelial-derived lipase
-
Moesziomyces antarcticus
GA 56 (enzyme)
-
Moesziomyces antarcticus
Gastric lipase
-
Moesziomyces antarcticus
GEH
-
Moesziomyces antarcticus
glycerol ester hydrolase
-
Moesziomyces antarcticus
glycerol-ester hydrolase
-
Moesziomyces antarcticus
heparin releasable hepatic lipase
-
Moesziomyces antarcticus
hepatic lipase
-
Moesziomyces antarcticus
hepatic monoacylglycerol acyltransferase
-
Moesziomyces antarcticus
Lingual lipase
-
Moesziomyces antarcticus
lipase
-
Geobacillus stearothermophilus
lipase
-
Aspergillus niger
lipase
-
Pseudomonas aeruginosa
lipase
-
Pseudomonas sp.
lipase
-
Acinetobacter calcoaceticus
lipase
-
Proteus vulgaris
lipase
-
Rhodotorula glutinis
lipase
-
Staphylococcus epidermidis
lipase
-
Rhizopus oryzae
lipase
-
Bacillus sp. (in: Bacteria)
lipase
-
Pseudomonas alcaligenes
lipase
-
Burkholderia cepacia
lipase
-
Aspergillus oryzae
lipase
-
Penicillium roqueforti
lipase
-
Pseudomonas oleovorans
lipase
-
Rhizomucor miehei
lipase
-
Moesziomyces antarcticus
lipase
-
Hyphopichia burtonii
lipase
-
Burkholderia sp.
lipase
-
Penicillium wortmanii
lipase, triacylglycerol
-
Moesziomyces antarcticus
lipazin
-
Moesziomyces antarcticus
liver lipase
-
Moesziomyces antarcticus
meito MY 30
-
Moesziomyces antarcticus
meito Sangyo OF lipase
-
Moesziomyces antarcticus
Pancreatic lipase
-
Moesziomyces antarcticus
Pancreatic lysophospholipase
-
Moesziomyces antarcticus
PGE
-
Moesziomyces antarcticus
PL-RP2
-
Moesziomyces antarcticus
post-heparin plasma protamine-resistant lipase
-
Moesziomyces antarcticus
PPL
-
Moesziomyces antarcticus
Pregastric esterase
-
Moesziomyces antarcticus
Pregastric lipase
-
Moesziomyces antarcticus
salt-resistant post-heparin lipase
-
Moesziomyces antarcticus
steapsin
-
Moesziomyces antarcticus
Sterol esterase
-
Moesziomyces antarcticus
takedo 1969-4-9
-
Moesziomyces antarcticus
teenesterase
-
Moesziomyces antarcticus
tiacetinase
-
Moesziomyces antarcticus
tibutyrin esterase
-
Moesziomyces antarcticus
triacylglycerol ester hydrolase
-
Moesziomyces antarcticus
Triacylglycerol lipase
-
Moesziomyces antarcticus
tributyrase
-
Moesziomyces antarcticus
tributyrinase
-
Moesziomyces antarcticus
triglyceridase
-
Moesziomyces antarcticus
triglyceride hydrolase
-
Moesziomyces antarcticus
triglyceride lipase
-
Moesziomyces antarcticus
triolein hydrolase
-
Moesziomyces antarcticus
tween hydrolase
-
Moesziomyces antarcticus
tween-hydrolyzing esterase
-
Moesziomyces antarcticus
Tweenase
-
Moesziomyces antarcticus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
extracellular enzyme
Rhizopus oryzae
40
-
strain F-111
Pseudomonas oleovorans
45
-
-
Hyphopichia burtonii
45
-
crude enzyme
Penicillium wortmanii
45
60
extracellular enzyme
Pseudomonas sp.
50
-
optimal growth temperature, strain RS-12, and extracellular enzyme
Bacillus sp. (in: Bacteria)
55
-
strain MB5001
Pseudomonas aeruginosa
60
-
enzyme from strain A30-1, also optimal growth temperature for the strain in vivo at pH 9.0
Bacillus sp. (in: Bacteria)
60
-
strain KW1-56
Pseudomonas sp.
60
65
L1
Geobacillus stearothermophilus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
50
strain RS-12, no growth at 40°C, maximal growth at 50°C
Bacillus sp. (in: Bacteria)
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
highly thermostable enzyme, strain A30-1
Bacillus sp. (in: Bacteria)
additional information
-
lipase B can be stabilized by covalent immobilization
Moesziomyces antarcticus
additional information
-
thermostable
Pseudomonas aeruginosa
additional information
-
thermostable enzyme
Geobacillus stearothermophilus
45
-
extracellular enzyme, 30 min, 65% remaining activity
Rhizopus oryzae
45
-
half-life 45 min
Rhodotorula glutinis
55
-
half-life 11.8 min
Rhodotorula glutinis
60
-
15 h, 90% remaining activity
Bacillus sp. (in: Bacteria)
60
-
extracellular enzyme, stable up to
Pseudomonas sp.
60
-
purified enzyme from strain KW1-56, 24 h, 96% remaining activity, thermostable enzyme
Pseudomonas sp.
75
-
after 30 min 100% remaining activity, after 8 h 50% remaining activity, extracellular enzyme shows 50% remaining activity after 15 min
Bacillus sp. (in: Bacteria)
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
native enzyme
Staphylococcus epidermidis
6.5
-
-
Hyphopichia burtonii
7
-
crude enzyme
Penicillium wortmanii
7
9
extracellular enzyme
Pseudomonas sp.
7.5
-
-
Rhodotorula glutinis
7.5
-
extracellular enzyme
Rhizopus oryzae
7.8
8.8
-
Acinetobacter calcoaceticus
8
-
strain MB5001
Pseudomonas aeruginosa
8.5
-
strain KKA-5
Pseudomonas aeruginosa
9
10
L1
Geobacillus stearothermophilus
9.5
-
enzyme from strain A30-1
Bacillus sp. (in: Bacteria)
10
-
-
Proteus vulgaris
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
strain F-111
Pseudomonas oleovorans
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas sp.
extracellular enzyme
-
4.5
Hyphopichia burtonii
isoelectric focusing
-
5.8
Pseudomonas aeruginosa
strain F-111
7.4
7.3
Rhizopus oryzae
extracellular enzyme
-
7.6
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
castor oil
at 2% as carbon source, enhances the enzyme production in vivo, pH 6.9
Pseudomonas aeruginosa
additional information
enzyme is not affected by benzamidine and PMSF
Rhizopus oryzae
additional information
the lipase of strain F-111 is unaffected by various detergents
Pseudomonas alcaligenes
Triton X-100
activation, strain F-111
Pseudomonas aeruginosa
Triton X-100
enhances the enzyme production in vivo by 50fold compared to olive oil alone in the medium
Rhizomucor miehei
Tween 80
activation, strain F-111
Pseudomonas aeruginosa
yeast extract
increases growth and enzyme production rate at 50°C, strain -12
Bacillus sp. (in: Bacteria)
Application (protein specific)
Application
Commentary
Organism
detergent
strain M1, enzyme is used for removal of fatty stains under conditions of a modern machine wash and in alkaline environment
Pseudomonas alcaligenes
paper production
removal of pitch, i.e. triglycerides and waxes, from the pulp produced for paper making
Diutina rugosa
synthesis
enantioselective hydrolysis of chiral esters
Pseudomonas aeruginosa
synthesis
immobilized enzyme is used for the transesterification reaction that replaces pamitic acid in palm oil with stearic acid
Rhizomucor miehei
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Geobacillus stearothermophilus
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Aspergillus niger
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Pseudomonas sp.
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Acinetobacter calcoaceticus
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Proteus vulgaris
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Rhodotorula glutinis
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Staphylococcus epidermidis
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Rhizopus oryzae
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Bacillus sp. (in: Bacteria)
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Burkholderia cepacia
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Aspergillus oryzae
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Penicillium roqueforti
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Diutina rugosa
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Moesziomyces antarcticus
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Hyphopichia burtonii
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Burkholderia sp.
synthesis
synthesis of enantiopure compounds by chemo-, regio-, and stereoselective transformations, catalysation of hydrolysis of water-immiscible triglycerides at water-liquid interface, transesterifications
Penicillium wortmanii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae, mature enzyme encoded by gene geh SE1 is overexpressed in Escherichia coli as N-terminally HIs-tagged enzyme
Staphylococcus epidermidis
gene tglA, enzyme form L3, DNA and amino acid sequence determination and analysis, functional expressionin Escherichia coli, Aspergillus oryzae is used as a host for expression of Thermomyces lanuginosus
Aspergillus oryzae
isozyme gene lip1, synthetically synthesized in an optimized nucleotide sequence to avoid the interference that occurs in heterologous expression of the native gene due to unusual codon usage, functional overexpression in Pichia pastoris of the synthetic gene, expression in Candida maltosa, a related yeast with the same codon usage, secretion in active form to the culture medium
Diutina rugosa
strain DSM 853, cloning and epitope mapping
Rhizopus oryzae
strain IGB83, cloning and functional expression in Xanthomonas campestris, secretion of the recombinant enzyme to the medium, expression of the enzyme also in Escherichia coli as His-tagged protein, secretion to the medium
Pseudomonas aeruginosa
strain M1, DNA sequencing and analysis, for functional expression in Escherichia coli the coexpression of a lipase-specific foldase, encoded by lipH, is required to ensure the correct folding and secretion of the recombinant enzyme through the host membrane
Pseudomonas alcaligenes
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
enzyme can be immobilized on Amberlite IRC50 with good long-term stability and high adsorption capacity
Rhizopus oryzae
additional information
immobilization of lipase B on Sepharose, alumina, silica, for the latter using precursors Si(OCH3)3 or Si(OCH3)4, providing a highly active, chemically and thermally stable, heterogeneous biocatalyst
Moesziomyces antarcticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
-
Penicillium roqueforti
Cd2+
strain KKA-5, slight inhibition
Pseudomonas aeruginosa
Cu2+
strong inhibition
Rhizopus oryzae
Cu2+
strain KKA-5, slight inhibition
Pseudomonas aeruginosa
Fe2+
-
Penicillium roqueforti
Fe2+
strong inhibition
Rhizopus oryzae
Fe2+
strain KKA-5, strong inhibition
Pseudomonas aeruginosa
Fe3+
strong inhibition
Rhizopus oryzae
Fe3+
60% inhibition at 1 mM
Pseudomonas oleovorans
Fe3+
strain KKA-5, strong inhibition
Pseudomonas aeruginosa
Hg2+
-
Penicillium roqueforti
Hg2+
strong inhibition
Rhizopus oryzae
Hg2+
strain KKA-5, strong inhibition
Pseudomonas aeruginosa
isopropyl fluorophosphate
-
Penicillium roqueforti
Mn2+
strain KKA-5, slight inhibition
Pseudomonas aeruginosa
additional information
enzyme from strain A30-1 is stable to alkaline protease treatment
Bacillus sp. (in: Bacteria)
additional information
enzyme is not affected by benzamidine and PMSF
Rhizopus oryzae
additional information
no inhibition by Ca2+, Hg2+, Zn2+, Mn2+, Cu2+, Mg2+, Co2+, Cd2+, Pb2+, EDTA, and o-phenanthrolin
Pseudomonas oleovorans
additional information
no inhibition by Ca2+, Mg2+, Mn2+, Na+, K+, Cu2+, EDTA, p-chloromercuribenzoate, and iodoacetate
Penicillium roqueforti
additional information
strain KKA-5, no inhibition by Ca2+ and Mg2+
Pseudomonas aeruginosa
additional information
the lipase of strain F-111 is unaffected by various detergents
Pseudomonas alcaligenes
oleic acid
inhibits enzyme production, product inhibition
Diutina rugosa
SDS
strain F-111
Pseudomonas aeruginosa
Zn2+
strain KKA-5, strong inhibition, strain MB5001: 94% inhibition at 1 mM
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
4-nitrophenyl laurate
-
Rhodotorula glutinis
2.7
-
4-nitrophenyl butyrate
-
Rhodotorula glutinis
12
-
4-nitrophenyl palmitate
-
Burkholderia cepacia
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Pseudomonas sp.
-
-
extracellular
-
Proteus vulgaris
-
-
extracellular
-
Pseudomonas oleovorans
-
-
extracellular
especially in a lypolytic strain
Rhizopus oryzae
-
-
extracellular
L1, L2, and L3
Aspergillus oryzae
-
-
extracellular
strain F-111
Pseudomonas alcaligenes
-
-
extracellular
strain KKA-5, strain MB5001, strain IGB83
Pseudomonas aeruginosa
-
-
intracellular
-
Rhizopus oryzae
5622
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
activation, extracellular enzyme
Bacillus sp. (in: Bacteria)
Ca2+
required
Acinetobacter calcoaceticus
Ca2+
activation, extracellular enzyme
Bacillus sp. (in: Bacteria)
Ca2+
increases enzyme expression
Bacillus sp. (in: Bacteria)
Ca2+
native enzyme, required for activity
Staphylococcus epidermidis
CaCl2
1.24fold stimulation at 1 mM
Pseudomonas aeruginosa
Co2+
required
Acinetobacter calcoaceticus
Cu2+
required
Acinetobacter calcoaceticus
Iron
increases enzyme expression
Bacillus sp. (in: Bacteria)
Mg2+
required
Acinetobacter calcoaceticus
Mg2+
enhances enzyme expression
Aspergillus niger
Mg2+
increases enzyme expression
Bacillus sp. (in: Bacteria)
Mg2+
required for strain F-111 and KKA-5, maximal enzyme production of strain KKA-5 at 0.8 M Mg2+
Pseudomonas oleovorans
additional information
Ca2+-concentration does not affect the enzyme production
Pseudomonas oleovorans
additional information
strain A30-1 requires a complexed medium which must contains diverse metal ions, overview
Bacillus sp. (in: Bacteria)
Na+
activation, extracellular enzyme
Bacillus sp. (in: Bacteria)
taurocholic acid
1.6fold stimulation at 200 mM
Pseudomonas aeruginosa
Tween 80
increases growth and enzyme production rate at 50°C, strain -12
Bacillus sp. (in: Bacteria)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
enzyme form L1
Aspergillus oryzae
25000
-
x * 25000, SDS-PAGE
Penicillium roqueforti
29000
-
x * 29000, enzyme of strain MB5001, SDS-PAGE, x * 32000, enzyme from strain F-111, SDS-PAGE, x * 30000, enzyme of strain KKA-5, SDS-PAGE
Pseudomonas aeruginosa
30000
-
x * 29000, enzyme of strain MB5001, SDS-PAGE, x * 32000, enzyme from strain F-111, SDS-PAGE, x * 30000, enzyme of strain KKA-5, SDS-PAGE
Pseudomonas aeruginosa
30000
-
x * 33000, enzyme from strain KM1-56, SDS-PAGE, x * 30000, extracellular enzyme, SDS-PAGE
Pseudomonas sp.
31000
-
1 * 31000, SDS-PAGE
Proteus vulgaris
32000
-
extracellular enzyme, gel filtration
Rhizopus oryzae
32000
-
x * 29000, enzyme of strain MB5001, SDS-PAGE, x * 32000, enzyme from strain F-111, SDS-PAGE, x * 30000, enzyme of strain KKA-5, SDS-PAGE
Pseudomonas aeruginosa
32000
-
x * 32000, strain BD 413, SDS-PAGE
Acinetobacter calcoaceticus
33000
-
x * 33000, enzyme from strain KM1-56, SDS-PAGE, x * 30000, extracellular enzyme, SDS-PAGE
Pseudomonas sp.
35000
-
1 * 35000 + 1 * 46000, recombinant enzyme, SDS-PAGE
Staphylococcus epidermidis
45000
-
1 * 45000, strain J33
Bacillus sp. (in: Bacteria)
46000
-
1 * 35000 + 1 * 46000, recombinant enzyme, SDS-PAGE
Staphylococcus epidermidis
51000
-
gel filtration
Hyphopichia burtonii
80000
90000
recombinant enzyme, gel filtration and native PAGE
Staphylococcus epidermidis
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
1,1,1-trichloroethane
enzyme catalyzes the regioselective hydrolysis of preacetylated monosaccharide derivatives in
Aspergillus niger
hexane
estrification of lactic acid and alcohols in hexane
Moesziomyces antarcticus
n-heptane
enzyme performs the hydrolysis of 4-nitrophenyl palmitate in n-heptane
Burkholderia cepacia
n-heptane
the purified enzyme is less active in dry n-heptane than the crude preparation, but the addition of a small amount of water dramatically activates the purified enzyme but not the crude one
Diutina rugosa
toluene
catalyzes the esterification of sulcatol and fatty acids in toluene
Diutina rugosa
Oxidation Stability (protein specific)
Oxidation Stability
Organism
enzyme from strain A30-1 is stable to H2O2
Bacillus sp. (in: Bacteria)
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
strain 9 contains an enzyme which is organized as a preproenzyme
Staphylococcus epidermidis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Proteus vulgaris
enzyme form L1, to homogeneity
Aspergillus oryzae
extracellular enzyme, 1200fold
Rhizopus oryzae
from strain KW1-56: 13.9fold, extracellular enzyme: 37fold to homogeneity
Pseudomonas sp.
from strain MB5001 in a 3-step procedure, from strain F-111 to homogeneity, 518fold from strain KKA-5 to homogeneity
Pseudomonas aeruginosa
native enzyme to homogeneity, recombinant enzyme rROL from Saccharomyces cerevisiae, recombinant His-tagged mature enzyme
Staphylococcus epidermidis
partial from strain A30-1, 175fold from strain J33
Bacillus sp. (in: Bacteria)
strain BD413, to homogeneity
Acinetobacter calcoaceticus
to homogeneity
Penicillium roqueforti
to homogeneity
Hyphopichia burtonii
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
batch or fed-batch culture in a medium containing citric acid and soybean oil as substrates
Pseudomonas alcaligenes
-
cell culture
enzyme production in presence of 1% olive oil or corn oil in the culture medium
Bacillus sp. (in: Bacteria)
-
cell culture
submerged batch culture
Diutina rugosa
-
additional information
culture conditions, the extracellular enzyme is dependent on a special nitrogen source in the medium, e.g. peptone, while the intracellular enzyme form is not
Rhizopus oryzae
-
additional information
high enzyme production
Penicillium wortmanii
-
additional information
medium conditions for the cell culture
Pseudomonas aeruginosa
-
additional information
medium conditions for the cell culture
Pseudomonas oleovorans
-
additional information
strain A30-1 requires a complexed medium which must contains diverse metal ions, overview
Bacillus sp. (in: Bacteria)
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-nitrophenyl butyrate + H2O
-
650654
Rhodotorula glutinis
4-nitrophenol + butyrate
-
650654
Rhodotorula glutinis
?
4-nitrophenyl caprylate + H2O
L1, best substrate
650654
Geobacillus stearothermophilus
4-nitrophenol + caprylate
-
650654
Geobacillus stearothermophilus
?
4-nitrophenyl caprylate + H2O
preferred substrate of lipase A
650654
Diutina rugosa
4-nitrophenol + caprylate
-
650654
Diutina rugosa
?
4-nitrophenyl caprylate + H2O
L1, best substrate
650654
Geobacillus stearothermophilus L1
4-nitrophenol + caprylate
-
650654
Geobacillus stearothermophilus L1
?
4-nitrophenyl laurate + H2O
-
650654
Rhodotorula glutinis
4-nitrophenol + laurate
-
650654
Rhodotorula glutinis
?
4-nitrophenyl laurate + H2O
preferred substrate of lipase B
650654
Diutina rugosa
4-nitrophenol + laurate
-
650654
Diutina rugosa
?
4-nitrophenyl laurate + H2O
purified enzyme of strain F-111 shows preference for
650654
Pseudomonas aeruginosa
4-nitrophenol + laurate
-
650654
Pseudomonas aeruginosa
?
4-nitrophenyl myristate + H2O
purified enzyme of strain F-111 shows preference for
650654
Pseudomonas aeruginosa
4-nitrophenol + myristate
-
650654
Pseudomonas aeruginosa
?
4-nitrophenyl palmitate + H2O
-
650654
Acinetobacter calcoaceticus
4-nitrophenol + palmitate
-
650654
Acinetobacter calcoaceticus
?
4-nitrophenyl palmitate + H2O
in n-heptane
650654
Burkholderia cepacia
4-nitrophenol + palmitate
-
650654
Burkholderia cepacia
?
4-nitrophenyl palmitate + H2O
-
650654
Acinetobacter calcoaceticus BD 413
4-nitrophenol + palmitate
-
650654
Acinetobacter calcoaceticus BD 413
?
castor oil + H2O
-
650654
Pseudomonas aeruginosa
?
-
650654
Pseudomonas aeruginosa
?
methyl beta-D-glucopyranoside + H2O
regioselective deacetylation of preacylated substrate
650654
Aspergillus niger
methanol + beta-D-glucose
-
650654
Aspergillus niger
?
additional information
enantioselective transesterification of 2-phenoxy-1-propanol
650654
Pseudomonas sp.
?
-
650654
Pseudomonas sp.
?
additional information
highly specific for short-chain fatty acids esters
650654
Penicillium roqueforti
?
-
650654
Penicillium roqueforti
?
additional information
immobilized enzyme performs the transesterification reaction that replaces pamitic acid in palm oil with stearic acid
650654
Rhizomucor miehei
?
-
650654
Rhizomucor miehei
?
additional information
lipases A and B display no interfacial activation due to absence of the lid structure which regulates the access to the active site. The enzyme performs estrification of lactic acid and alcohols in hexane
650654
Moesziomyces antarcticus
?
-
650654
Moesziomyces antarcticus
?
additional information
strain A30-1, active with triglycerides of C16:0 to C22:0fatty acids and on natural fats and oils
650654
Bacillus sp. (in: Bacteria)
?
-
650654
Bacillus sp. (in: Bacteria)
?
additional information
substrates are long acyl chain 4-nitrophenol esters
650654
Acinetobacter calcoaceticus
?
-
650654
Acinetobacter calcoaceticus
?
additional information
highly specific for short-chain fatty acids esters
650654
Penicillium roqueforti IAM7268
?
-
650654
Penicillium roqueforti IAM7268
?
additional information
strain A30-1, active with triglycerides of C16:0 to C22:0fatty acids and on natural fats and oils
650654
Bacillus sp. (in: Bacteria) J33
?
-
650654
Bacillus sp. (in: Bacteria) J33
?
additional information
substrates are long acyl chain 4-nitrophenol esters
650654
Acinetobacter calcoaceticus BD 413
?
-
650654
Acinetobacter calcoaceticus BD 413
?
additional information
enantioselective transesterification of 2-phenoxy-1-propanol
650654
Pseudomonas sp. KM1-56
?
-
650654
Pseudomonas sp. KM1-56
?
R-ketoprofen + H2O
lipase B is stereoselective for the R-isomer in an antichiral solvent such as isopentyl methyl ketone or S(+)-carvone
650654
Moesziomyces antarcticus
?
-
650654
Moesziomyces antarcticus
?
rac 2-(4-chlorophenoxy)propanoic acid + n-butanol
esterification reaction, in n-heptane
650654
Diutina rugosa
?
-
650654
Diutina rugosa
?
sulcatol + fatty acid
esterification reaction, in toluene
650654
Diutina rugosa
?
-
650654
Diutina rugosa
?
tert-butyl octanoate + H2O
preferred substrate, high activity with
650654
Burkholderia sp.
tert-butanol + octanoate
-
650654
Burkholderia sp.
?
tributyrin + H2O
-
650654
Geobacillus stearothermophilus
dibutyrin + butyrate
-
650654
Geobacillus stearothermophilus
?
tributyrin + H2O
-
650654
Aspergillus niger
dibutyrin + butyrate
-
650654
Aspergillus niger
?
tributyrin + H2O
-
650654
Pseudomonas aeruginosa
dibutyrin + butyrate
-
650654
Pseudomonas aeruginosa
?
tributyrin + H2O
-
650654
Pseudomonas sp.
dibutyrin + butyrate
-
650654
Pseudomonas sp.
?
tributyrin + H2O
-
650654
Acinetobacter calcoaceticus
dibutyrin + butyrate
-
650654
Acinetobacter calcoaceticus
?
tributyrin + H2O
-
650654
Proteus vulgaris
dibutyrin + butyrate
-
650654
Proteus vulgaris
?
tributyrin + H2O
-
650654
Rhodotorula glutinis
dibutyrin + butyrate
-
650654
Rhodotorula glutinis
?
tributyrin + H2O
-
650654
Staphylococcus epidermidis
dibutyrin + butyrate
-
650654
Staphylococcus epidermidis
?
tributyrin + H2O
-
650654
Rhizopus oryzae
dibutyrin + butyrate
-
650654
Rhizopus oryzae
?
tributyrin + H2O
-
650654
Bacillus sp. (in: Bacteria)
dibutyrin + butyrate
-
650654
Bacillus sp. (in: Bacteria)
?
tributyrin + H2O
-
650654
Pseudomonas alcaligenes
dibutyrin + butyrate
-
650654
Pseudomonas alcaligenes
?
tributyrin + H2O
-
650654
Burkholderia cepacia
dibutyrin + butyrate
-
650654
Burkholderia cepacia
?
tributyrin + H2O
-
650654
Aspergillus oryzae
dibutyrin + butyrate
-
650654
Aspergillus oryzae
?
tributyrin + H2O
-
650654
Penicillium roqueforti
dibutyrin + butyrate
-
650654
Penicillium roqueforti
?
tributyrin + H2O
-
650654
Diutina rugosa
dibutyrin + butyrate
-
650654
Diutina rugosa
?
tributyrin + H2O
-
650654
Pseudomonas oleovorans
dibutyrin + butyrate
-
650654
Pseudomonas oleovorans
?
tributyrin + H2O
-
650654
Rhizomucor miehei
dibutyrin + butyrate
-
650654
Rhizomucor miehei
?
tributyrin + H2O
-
650654
Moesziomyces antarcticus
dibutyrin + butyrate
-
650654
Moesziomyces antarcticus
?
tributyrin + H2O
-
650654
Hyphopichia burtonii
dibutyrin + butyrate
-
650654
Hyphopichia burtonii
?
tributyrin + H2O
-
650654
Burkholderia sp.
dibutyrin + butyrate
-
650654
Burkholderia sp.
?
tributyrin + H2O
-
650654
Penicillium wortmanii
dibutyrin + butyrate
-
650654
Penicillium wortmanii
?
tributyrin + H2O
-
650654
Penicillium roqueforti IAM7268
dibutyrin + butyrate
-
650654
Penicillium roqueforti IAM7268
?
tributyrin + H2O
-
650654
Bacillus sp. (in: Bacteria) J33
dibutyrin + butyrate
-
650654
Bacillus sp. (in: Bacteria) J33
?
tributyrin + H2O
-
650654
Acinetobacter calcoaceticus BD 413
dibutyrin + butyrate
-
650654
Acinetobacter calcoaceticus BD 413
?
tributyrin + H2O
-
650654
Geobacillus stearothermophilus L1
dibutyrin + butyrate
-
650654
Geobacillus stearothermophilus L1
?
tributyrin + H2O
-
650654
Pseudomonas sp. KM1-56
dibutyrin + butyrate
-
650654
Pseudomonas sp. KM1-56
?
triolein + H2O
hydrolysis at all positions, strain J33
650654
Bacillus sp. (in: Bacteria)
diolein + oleate
-
650654
Bacillus sp. (in: Bacteria)
?
triolein + H2O
preferred substrate of enzyme form L1
650654
Aspergillus oryzae
diolein + oleate
-
650654
Aspergillus oryzae
?
triolein + H2O
hydrolysis at all positions, strain J33
650654
Bacillus sp. (in: Bacteria) J33
diolein + oleate
-
650654
Bacillus sp. (in: Bacteria) J33
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 25000, SDS-PAGE
Penicillium roqueforti
?
1 * 31000, SDS-PAGE
Proteus vulgaris
?
x * 29000, enzyme of strain MB5001, SDS-PAGE, x * 32000, enzyme from strain F-111, SDS-PAGE, x * 30000, enzyme of strain KKA-5, SDS-PAGE
Pseudomonas aeruginosa
?
x * 32000, strain BD 413, SDS-PAGE
Acinetobacter calcoaceticus
?
x * 33000, enzyme from strain KM1-56, SDS-PAGE, x * 30000, extracellular enzyme, SDS-PAGE
Pseudomonas sp.
dimer
1 * 35000 + 1 * 46000, recombinant enzyme, SDS-PAGE
Staphylococcus epidermidis
monomer
1 * 51000, SDS-PAGE
Hyphopichia burtonii
monomer
1 * 24000, enzyme form L1
Aspergillus oryzae
monomer
1 * 32000, extracellular enzyme, SDS-PAGE
Rhizopus oryzae
monomer
1 * 45000, strain J33
Bacillus sp. (in: Bacteria)
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
extracellular enzyme
Rhizopus oryzae
40
-
strain F-111
Pseudomonas oleovorans
45
-
-
Hyphopichia burtonii
45
-
crude enzyme
Penicillium wortmanii
45
60
extracellular enzyme
Pseudomonas sp.
50
-
optimal growth temperature, strain RS-12, and extracellular enzyme
Bacillus sp. (in: Bacteria)
55
-
strain MB5001
Pseudomonas aeruginosa
60
-
enzyme from strain A30-1, also optimal growth temperature for the strain in vivo at pH 9.0
Bacillus sp. (in: Bacteria)
60
-
strain KW1-56
Pseudomonas sp.
60
65
L1
Geobacillus stearothermophilus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
50
strain RS-12, no growth at 40°C, maximal growth at 50°C
Bacillus sp. (in: Bacteria)
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
highly thermostable enzyme, strain A30-1
Bacillus sp. (in: Bacteria)
additional information
-
lipase B can be stabilized by covalent immobilization
Moesziomyces antarcticus
additional information
-
thermostable
Pseudomonas aeruginosa
additional information
-
thermostable enzyme
Geobacillus stearothermophilus
45
-
extracellular enzyme, 30 min, 65% remaining activity
Rhizopus oryzae
45
-
half-life 45 min
Rhodotorula glutinis
55
-
half-life 11.8 min
Rhodotorula glutinis
60
-
15 h, 90% remaining activity
Bacillus sp. (in: Bacteria)
60
-
extracellular enzyme, stable up to
Pseudomonas sp.
60
-
purified enzyme from strain KW1-56, 24 h, 96% remaining activity, thermostable enzyme
Pseudomonas sp.
75
-
after 30 min 100% remaining activity, after 8 h 50% remaining activity, extracellular enzyme shows 50% remaining activity after 15 min
Bacillus sp. (in: Bacteria)
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
native enzyme
Staphylococcus epidermidis
6.5
-
-
Hyphopichia burtonii
7
-
crude enzyme
Penicillium wortmanii
7
9
extracellular enzyme
Pseudomonas sp.
7.5
-
-
Rhodotorula glutinis
7.5
-
extracellular enzyme
Rhizopus oryzae
7.8
8.8
-
Acinetobacter calcoaceticus
8
-
strain MB5001
Pseudomonas aeruginosa
8.5
-
strain KKA-5
Pseudomonas aeruginosa
9
10
L1
Geobacillus stearothermophilus
9.5
-
enzyme from strain A30-1
Bacillus sp. (in: Bacteria)
10
-
-
Proteus vulgaris
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
strain F-111
Pseudomonas oleovorans
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas sp.
extracellular enzyme
-
4.5
Hyphopichia burtonii
isoelectric focusing
-
5.8
Pseudomonas aeruginosa
strain F-111
7.4
7.3
Rhizopus oryzae
extracellular enzyme
-
7.6
Other publictions for EC 3.1.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741707
Li
Enhancing the thermostability ...
Rhizomucor miehei
Appl. Environ. Microbiol.
84
e02129-17
2018
-
-
1
-
2
-
-
3
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
3
-
5
3
2
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
5
3
2
-
-
-
-
-
-
-
3
3
742397
Manan
Enzymatic esterification of e ...
Rhizomucor miehei
Enzyme Microb. Technol.
108
42-52
2018
-
1
-
-
-
-
1
2
-
-
-
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
749967
Belhaj
Galactolipase activity of Tal ...
Thermomyces dupontii, Thermomyces dupontii Stolk
Biochim. Biophys. Acta
1863
1006-1015
2018
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
1
-
-
-
4
-
10
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
10
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
734495
Choi
-
Purification and characterizat ...
Saccharolobus solfataricus, Saccharolobus solfataricus DSM 1616
J. Mol. Catal. B
124
11-19
2016
-
-
-
-
-
-
4
3
-
-
1
-
-
4
-
-
1
-
-
-
1
-
10
1
-
1
-
2
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
-
1
-
-
-
-
1
-
-
1
-
10
1
1
-
2
3
1
-
-
-
-
-
-
-
3
3
742133
Tong
Characterization of a new sn- ...
Malbranchea cinnamomea
Biotechnol. Appl. Biochem.
63
471-478
2016
-
-
1
-
-
-
2
-
-
-
-
-
1
11
-
-
-
-
-
-
-
-
7
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
743110
Firdaus
-
Kinetic model of biodiesel pr ...
Thermomyces lanuginosus
J. Mol. Catal. B
133
55-64
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729356
Branco
Improving the thermostability ...
Pyrococcus furiosus
Biomed Res. Int.
2015
250532
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
11
-
-
1
-
-
-
-
-
1
-
1
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
729477
Kumari
Functional characterization of ...
Trichosporon asahii, Trichosporon asahii MSR54
Biotechnol. Lett.
37
121-130
2015
-
-
1
-
-
-
1
-
-
1
1
2
-
14
-
-
1
-
-
-
-
-
6
1
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
1
2
-
-
-
1
-
-
-
-
6
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
741647
Zhang
Heterologous expression of an ...
Thermomyces dupontii
Appl. Biochem. Biotechnol.
176
1722-1735
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
743102
Babaki
-
Effect of water, organic solv ...
Moesziomyces antarcticus, Thermomyces lanuginosus, Rhizomucor miehei
J. Mol. Catal. B
120
93-99
2015
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3
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3
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2
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3
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2
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-
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-
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-
-
-
-
-
-
727566
Li
Characterization of an organic ...
Haloarcula sp., Haloarcula sp. G41
Folia Microbiol. (Praha)
59
455-463
2014
-
1
-
-
-
1
7
-
1
3
1
-
15
11
-
-
1
-
-
1
-
-
14
1
-
1
1
2
-
1
1
1
-
-
-
-
-
1
-
-
-
-
1
-
7
-
-
1
3
1
-
15
-
-
1
-
1
-
-
14
1
1
1
2
-
1
1
1
-
-
-
-
-
-
-
728937
Xie
Identification of a novel phos ...
Homo sapiens
Am. J. Physiol. Endocrinol. Metab.
306
E1449-E1459
2014
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
2
-
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1
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2
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-
-
-
-
-
-
-
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-
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1
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1
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1
-
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2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729008
Bispo
Preparation of triacylglycerol ...
Rhizomucor miehei
Appl. Biochem. Biotechnol.
172
1866-1881
2014
-
1
-
-
-
-
-
-
-
-
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1
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9
-
-
-
-
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4
-
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1
-
2
-
-
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-
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-
-
-
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1
-
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-
-
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1
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-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729305
Aloulou
Kinetic and structural charact ...
Equus caballus, Homo sapiens, Sus scrofa
Biochim. Biophys. Acta
1841
581-587
2014
-
-
3
-
-
-
-
-
-
-
3
3
-
9
-
-
3
-
-
3
-
-
12
3
6
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
3
-
3
-
-
12
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729323
Gao
Site-directed mutagenesis stud ...
no activity in Malassezia globosa
Biochimie
102
29-36
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
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-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729337
Kratky
Pleiotropic regulation of mito ...
Mus musculus
Biochimie
96
106-112
2014
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
10
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
10
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
729771
Wilcox
The modulation of pancreatic l ...
Sus scrofa
Food Chem.
146
479-484
2014
-
-
-
-
-
-
1
-
-
-
-
-
-
7
-
-
-
-
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1
-
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2
-
-
-
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-
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-
-
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-
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1
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-
-
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-
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730046
Koch
Modifications of the C terminu ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
J. Biol. Chem.
289
19306-19316
2014
-
-
-
-
-
-
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2
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2
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78
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-
-
-
-
-
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2
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
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-
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2
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2
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730435
Loo
Mycelium-bound lipase from a l ...
Geotrichum candidum, Geotrichum candidum IMI 387428
Molecules
19
8556-8570
2014
-
-
-
-
-
-
-
-
-
-
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2
-
7
-
-
-
-
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1
-
2
6
-
3
-
-
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-
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-
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2
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1
-
2
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743363
Calero
Selective ethanolysis of sunf ...
Rhizomucor miehei
New Biotechnol.
31
596-601
2014
-
1
-
-
-
-
-
-
-
-
-
-
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13
-
-
-
-
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9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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748394
Roussel
A cutinase from Trichoderma r ...
Trichoderma reesei, Trichoderma reesei QM6a
J. Mol. Biol.
426
3757-3772
2014
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1
1
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2
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1
1
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3
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1
1
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1
-
-
-
6
-
-
-
-
2
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
1
1
-
-
-
1
1
1
-
-
-
6
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-
729064
Lu
Characterization of an extrace ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Appl. Microbiol. Biotechnol.
97
2443-2454
2013
1
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-
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10
-
1
3
1
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51
-
-
-
-
-
-
-
-
4
1
3
1
1
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
10
-
-
1
3
1
-
-
-
-
-
-
-
-
-
4
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
730017
Schmidt
Regulation of the yeast triacy ...
Saccharomyces cerevisiae
J. Biol. Chem.
288
19939-19948
2013
-
-
-
-
-
-
-
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4
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-
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6
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-
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-
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1
-
-
1
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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4
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
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1
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730027
Ploier
Screening for hydrolytic enzym ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae TM
J. Biol. Chem.
288
36061-36072
2013
-
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-
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1
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1
2
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9
-
-
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-
-
-
-
-
6
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
1
2
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730743
Bou Ali
N-terminal domain of turkey pa ...
Sus scrofa
PLoS ONE
8
e71605
2013
-
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1
-
-
-
1
-
-
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1
-
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6
-
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1
-
-
3
2
-
3
1
3
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
3
2
-
3
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
730877
Vijayakumar
Rice (Oryza sativa) lipase: mo ...
Oryza sativa
Protein Expr. Purif.
88
67-79
2013
-
-
1
-
-
-
-
4
-
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2
-
-
10
-
-
1
-
1
-
2
-
13
2
3
1
-
-
4
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
1
1
-
2
-
13
2
1
-
-
4
1
-
1
-
-
-
-
-
-
-
742098
Martins
Improved production of butyl ...
Thermomyces lanuginosus
Biores. Technol.
134
417-422
2013
-
1
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-
-
-
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6
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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714489
Cho
A neutral lipase applicable in ...
Streptomyces sp., Streptomyces sp. CS326
Bioprocess Biosyst. Eng.
35
:227-234
2012
-
1
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1
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1
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7
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1
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-
-
-
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2
1
2
1
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1
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1
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1
-
-
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-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
2
1
1
-
1
-
1
-
1
-
-
-
-
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-
-
716181
Sharma
Characterization of a thermost ...
uncultured bacterium
Mol. Biol. Rep.
39
2795-2804
2012
-
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1
-
-
-
10
1
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4
1
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2
5
-
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1
-
-
-
2
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7
1
1
1
-
1
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
10
-
1
-
4
1
-
2
-
-
1
-
-
2
-
7
1
1
-
1
1
1
-
1
-
-
-
-
-
-
-
729345
Radovic
Adipose triglyceride lipase in ...
Mus musculus
Biol. Chem.
393
1005-1011
2012
1
-
-
-
-
-
-
-
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1
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1
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-
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3
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
729462
Kumari
Extracellular expression and c ...
Yarrowia lipolytica, Yarrowia lipolytica MSR80
Biotechnol. Lett.
34
1733-1739
2012
6
-
3
-
-
-
7
-
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6
-
-
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15
-
-
-
-
-
-
-
-
48
-
3
3
-
3
-
3
-
-
-
-
-
-
6
-
3
-
-
-
-
-
21
-
-
-
6
-
-
-
-
-
-
-
-
-
-
48
-
3
-
3
-
3
-
-
-
-
-
-
-
-
-
729664
Li
Rapid triacylglycerol turnover ...
Chlamydomonas reinhardtii, Chlamydomonas reinhardtii dw15.1
Eukaryot. Cell
11
1451-1462
2012
-
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1
-
-
-
-
-
-
-
1
2
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16
-
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1
-
-
-
-
-
12
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
12
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730002
Eichmann
Studies on the substrate and s ...
Mus musculus
J. Biol. Chem.
287
41446-41457
2012
1
-
1
-
-
-
-
-
-
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4
-
1
-
-
-
-
-
-
-
-
11
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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730157
Attya
Endogenous lipase catalyzed tr ...
Rhizomucor miehei
J. Mass Spectrom.
47
1247-1253
2012
-
-
-
-
-
-
-
-
-
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1
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1
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-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730703
Chen
In silico identification of po ...
Homo sapiens
PLoS ONE
7
e43932
2012
-
-
-
-
-
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4
-
-
-
-
-
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4
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-
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2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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4
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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742676
Romdhan
Purification, physico-chemica ...
Thermomyces dupontii
Int. J. Biol. Macromol.
51
892-900
2012
-
-
-
-
-
-
-
-
-
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-
-
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6
-
1
1
-
-
-
4
-
3
1
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
4
-
3
1
2
-
-
-
1
-
-
-
-
1
1
-
-
-
713778
Kamijo
Molecular and enzymatic charac ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) HH-01
Antonie van Leeuwenhoek
99
179-187
2011
-
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1
-
-
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4
-
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1
2
-
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16
-
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1
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-
-
-
-
13
2
2
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
1
2
-
-
-
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1
-
-
-
-
13
2
1
-
1
-
1
-
1
-
2
-
-
2
-
-
713807
Peng
Co-expression of an organic so ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa CS-2
Appl. Biochem. Biotechnol.
165
926-937
2011
-
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1
-
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1
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-
-
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1
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1
13
-
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1
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-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
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1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713808
Shangguan
Expression and characterizatio ...
Aspergillus fumigatus, Aspergillus fumigatus CGMCC 2873
Appl. Biochem. Biotechnol.
165
949-962
2011
3
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1
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5
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7
1
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1
1
-
6
10
-
-
1
-
-
1
2
-
13
1
2
1
-
1
-
1
1
1
-
-
-
-
3
-
1
-
-
5
-
-
7
-
1
-
1
1
-
6
-
-
1
-
1
2
-
13
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
713893
Cai
Fervidobacterium changbaicum L ...
Fervidobacterium changbaicum, Fervidobacterium changbaicum CBS-1
Appl. Microbiol. Biotechnol.
89
1463-1473
2011
-
-
-
-
-
-
-
1
-
-
-
-
-
16
-
-
-
-
-
-
-
-
4
-
1
1
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
1
1
1
-
1
-
-
-
-
-
-
-
714000
Yesiloglu
Biochemical properties of free ...
Diutina rugosa
Artif. Cells Blood Substit. Immobil. Biotechnol.
39
247-251
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
2
1
-
1
2
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
2
-
1
-
2
-
-
-
-
-
-
-
-
-
714072
Bouaziz
-
Expression, purification of a ...
Staphylococcus xylosus
Biochem. Eng. J.
54
93-102
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
4
-
2
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
2
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
714501
Dheeman
Purification and properties of ...
Amycolatopsis mediterranei, Amycolatopsis mediterranei DSM 43304
Biores. Technol.
102
3373-3379
2011
5
-
-
-
-
-
7
1
-
-
1
-
-
13
-
-
1
-
-
-
2
-
21
1
1
1
-
1
1
1
1
1
-
-
-
-
5
-
-
-
-
-
-
-
7
-
1
-
-
1
-
-
-
-
1
-
-
2
-
21
1
1
-
1
1
1
1
1
-
-
-
-
-
-
-
714505
Acosta
Kinetically controlled synthes ...
Rhizomucor miehei
Biores. Technol.
102
507-512
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
5
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
1
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
714509
Ebrahimpour
High level expression and char ...
Geobacillus sp., Geobacillus sp. ARM
Biores. Technol.
102
6972-6981
2011
3
-
1
-
-
1
30
-
-
2
1
-
19
8
-
-
1
-
-
3
2
-
4
2
1
1
-
-
-
1
-
1
-
-
-
-
3
-
1
-
-
-
1
-
30
-
-
-
2
1
-
19
-
-
1
-
3
2
-
4
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
714583
Zhao
Cloning, expression and charac ...
Yarrowia lipolytica, Yarrowia lipolytica CLIB 122
Biotechnol. Lett.
33
2445-2452
2011
-
-
1
-
-
-
10
-
-
1
-
-
-
14
-
-
-
-
-
-
-
-
17
-
2
1
1
1
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
10
-
-
-
1
-
-
-
-
-
-
-
-
-
-
17
-
1
1
1
-
1
1
1
-
-
-
-
-
-
-
714773
Rivera-Perez
Purification and characterizat ...
Penaeus vannamei
Comp. Biochem. Physiol. B
158
99-105
2011
-
-
-
-
-
-
2
1
6
1
3
-
-
12
-
1
1
-
-
2
8
-
7
1
1
1
1
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
2
-
1
6
1
3
-
-
-
1
1
-
2
8
-
7
1
1
1
-
1
-
1
-
1
-
1
1
-
1
1
714870
Miranda
Assessment of the morphologica ...
Diutina rugosa
Enzyme Res.
2011
216435
2011
-
1
-
-
-
1
1
2
-
-
-
-
-
6
-
-
-
-
-
-
-
1
2
-
1
2
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
1
2
-
2
-
1
-
2
-
-
-
-
-
-
-
-
-
715206
Lan
A novel cold-active lipase fro ...
Candida albicans, Candida albicans ATCC 10231
Int. J. Mol. Sci.
12
3950-3965
2011
-
-
1
-
3
-
16
4
-
1
1
-
4
21
-
-
1
-
-
-
-
-
10
1
2
1
1
1
4
1
-
1
-
-
-
-
-
-
1
-
-
3
-
-
16
-
4
-
1
1
-
4
-
-
1
-
-
-
-
10
1
1
1
1
4
1
-
1
-
-
-
-
-
4
4
715236
Chang
Engineering the expression and ...
Diutina rugosa
J. Agric. Food Chem.
59
6710-6719
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
715683
Kaewprapan
Lipase-catalyzed synthesis of ...
Diutina rugosa
J. Biosci. Bioeng.
112
124-129
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
9
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715824
Christeller
Galactolipase, phospholipase a ...
Ephestia kuehniella, Galleria mellonella, Helicoverpa armigera, Plodia interpunctella, Epiphyas postvittana, Tineola bisselliella
J. Insect Physiol.
57
1232-1239
2011
-
6
-
-
-
-
-
-
-
-
-
-
-
29
-
-
-
-
-
18
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716082
Bou Ali
Purification and characterizat ...
Meleagris gallopavo
Lipids Health Dis.
10
24
2011
-
-
1
-
-
-
1
-
-
1
1
-
-
11
-
-
1
-
-
7
3
-
2
1
2
1
1
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
-
7
3
-
2
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
716176
Khurana
Engineering of Bacillus lipase ...
Bacillus sp. (in: Bacteria)
Mol. Biol. Rep.
38
2919-2926
2011
-
-
1
-
1
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
3
-
7
1
2
2
-
1
-
1
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
3
-
7
1
2
-
1
-
1
-
2
-
-
-
-
-
-
-
716527
Abdelkafi
Carica papaya lipase: a natura ...
Carica papaya
Plant Foods Hum. Nutr.
66
34-40
2011
-
-
-
-
-
1
4
-
-
-
-
-
-
10
-
-
-
-
-
1
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
742233
Verdugo
-
Production of a new second ge ...
Thermomyces lanuginosus
Catal. Today
167
107-112
2011
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708339
Chiu
Mice lacking hepatic lipase ar ...
Mus musculus
Endocrinology
151
993-1001
2010
1
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
713783
Uttatree
Isolation and characterization ...
Acinetobacter baylyi
Appl. Biochem. Biotechnol.
162
1362-1376
2010
1
-
-
-
-
-
13
-
-
3
1
-
12
12
-
-
1
-
-
-
2
-
9
1
1
1
1
1
-
1
1
1
-
-
-
-
1
-
-
-
-
-
-
-
13
-
-
-
3
1
-
12
-
-
1
-
-
2
-
9
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
713785
Smichi
Purification and biochemical c ...
Sardinella aurita
Appl. Biochem. Biotechnol.
162
1483-1496
2010
-
-
-
-
-
-
1
-
-
-
1
-
-
12
-
-
-
-
-
2
-
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713788
Wang
Significantly improved express ...
Serratia marcescens, Serratia marcescens ECU1010
Appl. Biochem. Biotechnol.
162
2387-2399
2010
2
1
1
-
-
-
5
1
-
6
1
-
-
12
-
-
1
-
-
-
2
-
13
1
1
1
1
1
-
1
-
1
-
-
-
-
2
1
1
-
-
-
-
-
5
-
1
-
6
1
-
-
-
-
1
-
-
2
-
13
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
714856
Nguyen
Enzymatic properties and expre ...
Fusarium graminearum
Enzyme Microb. Technol.
46
479-486
2010
-
-
1
-
-
-
-
-
1
-
5
-
-
8
-
-
-
-
-
-
-
-
10
5
6
1
2
-
-
2
1
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
-
10
5
1
2
-
-
2
1
-
5
-
-
-
-
-
-
715063
Kurtovic
Purification and properties of ...
Oncorhynchus tshawytscha, Macruronus novaezelandiae
Fish Physiol. Biochem.
36
1041-1060
2010
4
-
-
-
-
-
18
2
-
4
3
-
12
10
-
-
2
-
-
2
3
-
12
3
2
2
-
-
-
2
-
-
-
2
2
-
4
-
-
-
-
-
-
-
18
2
2
-
4
3
-
12
-
-
2
-
2
3
-
12
3
2
-
-
-
2
-
-
2
-
-
-
-
-
-
715282
Engstroem
Directed evolution of an enant ...
Moesziomyces antarcticus
J. Am. Chem. Soc.
132
7038-7042
2010
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715393
Dahiya
Characterization of an extrace ...
Pseudomonas mendocina, Pseudomonas mendocina M-37
J. Basic Microbiol.
50
420-426
2010
-
-
-
-
-
-
12
-
1
3
-
-
8
11
-
-
-
-
-
-
-
-
18
-
2
1
1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
1
12
-
-
1
3
-
-
8
-
-
-
-
-
-
-
18
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
715691
Brusamarelo
Kinetics of lipase-catalyzed s ...
Moesziomyces antarcticus
J. Biotechnol.
147
108-115
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715694
Juhl
Engineering of Candida antarct ...
Moesziomyces antarcticus
J. Biotechnol.
150
474-480
2010
-
-
1
-
10
-
-
-
-
-
1
-
-
8
-
-
1
-
-
-
1
-
15
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
15
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716191
Kolling
Biochemical and structural cha ...
Staphylococcus xylosus
Mol. Biotechnol.
46
168-175
2010
-
-
1
-
2
-
-
3
-
-
-
-
-
9
-
-
1
-
-
-
3
-
1
-
1
1
1
1
3
1
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
3
-
1
-
1
1
1
3
1
1
-
-
-
-
-
-
3
3
716567
Panzanaro
Biochemical characterization o ...
Olea europaea
Plant Physiol. Biochem.
48
741-745
2010
-
-
-
-
-
-
9
-
-
1
-
-
-
7
-
-
-
-
-
2
-
-
1
-
2
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
1
-
-
2
-
-
2
-
-
716868
Bian
Crystal structure of a triacyl ...
Penicillium expansum, Penicillium expansum PF898
Proteins Struct. Funct. Bioinform.
78
1601-1605
2010
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1
1
4
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4
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1
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1
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743701
Rodrigues
-
Two step ethanolysis A simpl ...
Thermomyces lanuginosus
Process Biochem.
45
1268-1273
2010
-
1
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-
-
-
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1
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1
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1
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743702
Raita
-
Biocatalytic ethanolysis of p ...
Thermomyces lanuginosus
Process Biochem.
45
829-834
2010
-
1
1
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1
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1
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1
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1
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1
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1
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690508
Dutta
Production and characterizatio ...
Bacillus cereus, Bacillus cereus C(7)
Appl. Biochem. Biotechnol.
159
142-154
2009
2
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1
14
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5
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2
8
1
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2
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1
1
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1
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1
2
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2
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1
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14
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5
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1
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2
-
1
-
1
-
1
2
-
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-
-
-
-
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690600
Tanino
Improvement of a Candida antar ...
Moesziomyces antarcticus
Appl. Microbiol. Biotechnol.
82
59-66
2009
-
-
1
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11
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1
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691320
Goodman
Discovery of potent, selective ...
Mus musculus
Bioorg. Med. Chem. Lett.
19
27-30
2009
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23
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1
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23
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23
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1
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691448
Xia
Wheat germ lipase catalyzed ki ...
Triticum aestivum
Biotechnol. Lett.
31
83-87
2009
-
-
-
-
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6
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1
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1
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1
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4
-
1
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-
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-
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691892
Jemel
Kinetic properties of dromedar ...
Camelus dromedarius
Colloids Surf. B Biointerfaces
70
238-242
2009
-
-
-
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2
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12
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5
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5
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2
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2
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5
-
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5
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2
-
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-
-
-
-
-
-
693798
Utsugi
Lipase specificity in the tran ...
Aspergillus niger, Burkholderia cepacia, Diutina rugosa, Mucor javanicus, Rhizomucor miehei, Penicillium camemberti, Penicillium roqueforti, Pseudomonas fluorescens, Rhizopus oryzae, Rhizopus niveus, Sus scrofa
J. Oleo Sci.
58
123-132
2009
-
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11
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1
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22
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12
12
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10
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1
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-
22
-
12
-
-
-
10
-
-
-
-
-
-
-
-
-
693957
Choo
Radical scavenging activity of ...
Moesziomyces antarcticus
Lipids
44
145-152
2009
-
-
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-
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-
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6
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2
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2
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-
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2
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-
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-
-
-
-
-
-
-
-
694128
Kurat
Cdk1/Cdc28-dependent activatio ...
Saccharomyces cerevisiae
Mol. Cell
33
53-63
2009
1
-
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-
-
-
-
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1
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9
-
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3
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1
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-
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-
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-
-
-
-
-
694139
Nie
cDNA cloning, characterization ...
Gallus gallus
Mol. Cell. Biochem.
320
67-74
2009
-
-
1
-
-
-
-
-
-
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1
1
-
14
-
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8
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1
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2
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1
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1
1
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8
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694611
Slanc
Screening of selected food and ...
Sus scrofa
Phytother. Res.
23
874-877
2009
-
-
-
-
-
-
1
-
-
-
-
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-
17
-
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-
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3
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2
-
2
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1
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3
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2
-
-
-
-
-
-
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-
-
-
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-
-
-
-
694984
Abedi Karjiban
Molecular dynamics study of th ...
Geobacillus stearothermophilus
Protein J.
28
14-23
2009
-
-
-
-
-
-
-
-
-
2
1
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-
3
-
-
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1
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2
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707268
Watt
Triglyceride lipases alter fue ...
Homo sapiens, Mus musculus
Appl. Physiol. Nutr. Metab.
34
340-347
2009
2
-
-
-
2
-
-
-
2
-
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2
-
4
-
-
-
-
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4
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2
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6
-
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2
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2
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2
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2
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-
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4
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
707500
Chatterjee
Hepatic high-density lipoprote ...
Homo sapiens
Biochemistry
48
5994-6001
2009
3
-
-
-
-
-
-
-
1
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-
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3
-
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3
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1
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3
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707547
Zimmermann
Fate of fat: the role of adipo ...
Homo sapiens, Mus musculus
Biochim. Biophys. Acta
1791
494-500
2009
4
-
-
-
2
-
-
-
2
-
-
2
-
4
-
-
-
-
-
18
-
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4
-
11
-
-
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-
-
-
-
-
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4
-
-
-
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2
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-
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-
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2
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2
-
-
-
-
-
18
-
-
4
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
708289
Liu
Regulation of adipose triglyce ...
Mus musculus
Diabetes Obes. Metab.
11
131-142
2009
-
-
-
-
-
-
-
-
-
-
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1
-
3
-
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-
-
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2
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1
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2
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1
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-
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2
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
709424
Zechner
Adipose triglyceride lipase an ...
Homo sapiens, Mus musculus
J. Lipid Res.
50
3-21
2009
2
-
2
-
2
-
-
-
3
-
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2
-
8
-
-
-
-
-
16
-
-
4
2
10
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
16
-
-
4
2
-
-
-
-
-
-
-
-
2
5
5
2
-
-
709885
Nie
Identification and characteriz ...
Anas platyrhynchos, Coturnix coturnix, Melopsittacus undulatus
Mol. Biol. Rep.
37
3487-3493
2009
-
-
3
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
54
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
54
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722957
Chen
Structure of the alkalohyperth ...
Archaeoglobus fulgidus
J. Mol. Biol.
390
672-685
2009
-
-
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1
1
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-
-
-
-
-
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12
-
-
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-
-
-
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3
-
-
2
1
2
-
2
1
3
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
1
2
-
2
1
3
-
-
-
-
-
-
-
679242
Ammazzalorso
Candida rugosa lipase-catalyse ...
Diutina rugosa
Chirality
20
115-118
2008
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
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4
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1
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1
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-
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-
-
-
-
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1
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-
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-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
680071
Jocken
Adipose triglyceride lipase (A ...
Homo sapiens
Histochem. Cell Biol.
129
535-538
2008
-
-
-
-
-
-
-
-
-
-
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1
-
4
-
-
-
-
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5
-
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1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690250
Carrasco-Lopez
Crystallization and preliminar ...
Geobacillus thermocatenulatus
Acta Crystallogr. Sect. F
64
1043-1045
2008
-
-
1
1
-
-
-
-
-
-
1
-
-
10
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690274
Sun
Crystallization and preliminar ...
Fusarium graminearum
Acta Crystallogr. Sect. F
64
813-815
2008
-
-
1
1
-
-
1
-
1
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690408
Saminathan
Blue native electrophoresis st ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa MTCC-2297
Anal. Biochem.
377
270-271
2008
-
1
-
-
-
-
-
-
1
-
-
2
1
8
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
1
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
690486
Liu
Cloning, characterization and ...
Aureobasidium pullulans, Aureobasidium pullulans HN2-3
Antonie van Leeuwenhoek
94
245-255
2008
-
-
1
-
-
-
-
-
1
-
2
-
-
25
-
-
-
-
-
-
4
-
10
-
1
1
1
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
4
-
10
-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
690489
Pinheiro
Response surface methodology a ...
Diutina rugosa
Appl. Biochem. Biotechnol.
146
203-214
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690493
Saisubramanian
Two step purification of Acine ...
Acinetobacter sp., Acinetobacter sp. MTCC 6816
Appl. Biochem. Biotechnol.
150
139-156
2008
-
-
-
-
-
-
-
-
-
-
1
-
2
7
-
-
1
-
-
-
2
-
4
-
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
2
-
4
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
690498
Gaur
Protein-coated microcrystals o ...
Pseudomonas aeruginosa
Appl. Biochem. Biotechnol.
151
160-166
2008
-
1
-
-
1
-
-
2
-
-
-
1
-
9
-
-
-
-
-
-
-
-
4
-
2
2
1
1
-
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
4
-
2
1
1
-
1
1
-
-
-
-
-
-
-
-
690670
Islam
-
Isolation, purification and ch ...
Liza parsia
Asian J. Biochem.
3
243-255
2008
-
-
-
-
-
-
6
-
-
2
2
-
-
1
-
-
1
-
-
-
2
-
2
1
2
2
1
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
2
2
-
-
-
-
1
-
-
2
-
2
1
2
1
1
-
2
1
1
-
-
-
-
-
-
-
690730
Colin
Exploring the active site cavi ...
Homo sapiens
Biochem. Biophys. Res. Commun.
370
394-398
2008
-
-
1
-
4
-
1
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
1
-
2
-
-
-
-
1
1
-
-
-
-
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-
-
1
-
-
4
-
-
1
-
-
-
-
-
-
-
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-
-
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
690784
Chew
-
Kinetic study on the hydrolysi ...
Thermomyces lanuginosus
Biochem. Eng. J.
39
516-520
2008
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
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-
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-
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2
-
2
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1
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690785
Liu
-
Production, purification and c ...
Aureobasidium pullulans, Aureobasidium pullulans HN2.3
Biochem. Eng. J.
40
445-451
2008
-
-
-
-
-
-
16
1
1
-
1
-
-
2
-
-
1
-
-
-
2
-
10
-
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
16
-
1
1
-
1
-
-
-
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1
-
-
2
-
10
-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
691095
Singh
Simultaneous refolding and pur ...
Burkholderia cepacia
Biochim. Biophys. Acta
1784
1825-1829
2008
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
1
-
1
-
1
-
1
-
1
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-
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-
1
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-
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2
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1
-
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1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691136
Schoffelen
Site-specific modification of ...
Moesziomyces antarcticus
Bioconjug. Chem.
19
1127-1131
2008
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
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-
-
2
-
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-
-
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-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691187
Fernandez-Lorente
Solid-phase chemical amination ...
Geobacillus thermocatenulatus
Biomacromolecules
9
2553-2561
2008
-
-
1
-
-
1
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
3
-
1
-
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1
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1
-
-
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-
1
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-
1
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-
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-
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-
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1
-
-
-
-
3
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
691189
Chen
Candida antarctica lipase B ch ...
Moesziomyces antarcticus
Biomacromolecules
9
463-471
2008
-
-
-
-
-
1
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
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1
-
2
-
-
-
-
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1
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1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691344
Kempka
Response surface method to opt ...
Penicillium verrucosum
Bioprocess Biosyst. Eng.
31
119-125
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
8
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
-
-
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
691346
Hilterhaus
Practical application of diffe ...
Moesziomyces antarcticus
Bioprocess Biosyst. Eng.
31
163-171
2008
-
1
-
-
-
1
-
-
-
-
1
2
-
5
-
-
-
-
-
1
3
-
2
-
2
-
-
-
-
-
-
-
-
-
-
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-
1
-
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-
-
1
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1
2
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-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691356
Sun
Membrane-bound synthetic lipas ...
Rhizopus microsporus var. chinensis
Bioresour. Technol.
100
1336-1342
2008
2
-
-
-
-
-
14
-
-
-
2
-
-
1
-
-
1
-
-
-
4
-
8
1
2
1
1
1
-
1
1
1
-
-
-
-
2
-
-
-
-
-
-
-
14
-
-
-
-
2
-
-
-
-
1
-
-
4
-
8
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
691357
Mhetras
Purification and characterizat ...
Aspergillus niger, Aspergillus niger NCIM 1207
Bioresour. Technol.
100
1486-1490
2008
-
-
-
-
-
-
6
-
1
-
2
-
-
7
-
-
1
-
-
-
-
-
4
1
1
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
-
1
-
2
-
-
-
-
1
-
-
-
-
4
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
691403
Joseph
Cold active microbial lipases: ...
Acinetobacter sp., Aeromonas sp., Aspergillus nidulans, Lysinibacillus sphaericus, Pseudomonas sp., Psychrobacter sp., Serratia marcescens, Pseudoalteromonas sp., Microbacterium phyllosphaerae, Microbacterium phyllosphaerae MTCC 7530, Psychrobacter sp. 7195, Pseudoalteromonas sp. wp27, Pseudomonas sp. KB700A, Aspergillus nidulans WG312, Lysinibacillus sphaericus MTCC 7526, Pseudomonas sp. B11-1, Psychrobacter sp. wp37, Aeromonas sp. LPB 4
Biotechnol. Adv.
26
457-470
2008
-
45
2
-
-
-
4
-
-
2
9
-
-
22
-
-
8
-
-
-
-
-
8
-
9
11
-
8
-
9
-
5
-
-
-
-
-
45
2
-
-
-
-
-
4
-
-
-
2
9
-
-
-
-
8
-
-
-
-
8
-
11
-
8
-
9
-
5
-
-
-
-
-
-
-
691405
Dalal
Purification and properties of ...
Burkholderia cepacia
Biotechnol. Appl. Biochem.
51
23-31
2008
1
-
1
-
-
-
-
2
-
-
1
-
2
7
-
-
1
-
-
-
2
-
2
-
1
1
-
1
-
1
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
2
-
-
1
-
2
-
-
1
-
-
2
-
2
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
691413
Secundo
Role of methoxypolyethylene gl ...
Burkholderia cepacia
Biotechnol. Bioeng.
101
255-262
2008
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
1
-
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-
-
-
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-
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-
-
1
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691439
Zoumpanioti
Esterification reactions catal ...
Rhizomucor miehei
Biotechnol. Lett.
30
1627-1631
2008
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
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-
-
1
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-
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-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691471
Park
Purification and characterizat ...
Todarodes pacificus
BMB Rep.
41
254-258
2008
-
-
-
-
-
-
8
-
-
-
1
-
-
7
-
-
1
-
-
4
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
1
-
-
-
-
1
-
4
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
691565
Paques
-
Characterization of the lipase ...
Carica papaya
Braz. J. Food Technol.
11
20-27
2008
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
2
3
-
1
-
1
1
-
3
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
2
3
-
1
-
1
-
3
-
4
-
1
-
-
-
-
-
-
-
691762
Xiong
-
Kinetic study of lipase cataly ...
Alcaligenes sp.
Chem. Eng. J.
138
258-263
2008
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
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-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
691764
Wei
-
Production and characteristics ...
Burkholderia sp., Burkholderia sp. GXU56
Chem. Eng. Technol.
31
258-264
2008
-
-
-
-
-
-
13
1
-
2
1
-
3
2
-
-
1
-
-
-
2
-
4
-
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
13
-
1
-
2
1
-
3
-
-
1
-
-
2
-
4
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
691889
Karra-Chaabouni
Physical immobilization of Rhi ...
Rhizopus oryzae
Colloids Surf. B Biointerfaces
66
168-177
2008
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
2
-
2
-
-
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
1
1
1
-
-
-
-
-
-
-
692121
Parra
-
Cloning and fusion expression ...
Psychrobacter sp.
Enzyme Microb. Technol.
42
371-377
2008
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
6
-
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
6
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
692122
Cote
-
Cloning, purification and char ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Enzyme Microb. Technol.
42
381-388
2008
-
-
-
-
-
-
7
-
-
-
2
-
-
4
-
-
2
-
-
-
-
-
26
-
4
2
-
1
-
-
2
1
-
-
2
-
-
-
-
-
-
-
-
-
14
-
-
-
-
2
-
-
-
-
2
-
-
-
-
26
-
2
-
1
-
-
2
1
2
-
-
-
-
-
-
692254
de Pascale
The cold-active Lip1 lipase fr ...
Pseudoalteromonas haloplanktis, Pseudoalteromonas haloplanktis TAC 125
Extremophiles
12
311-323
2008
-
-
1
-
-
-
11
-
1
-
-
-
-
12
-
-
1
-
-
-
2
-
12
-
1
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
11
-
-
1
-
-
-
-
-
-
1
-
-
2
-
12
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
692349
Gatti-Lafranconi
Unscrambling thermal stability ...
Pseudomonas fragi
FEBS Lett.
582
2313-2318
2008
-
-
1
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
692387
Hausmann
Catalytic mechanism of C-di-GM ...
Pseudomonas aeruginosa
FEMS Microbiol. Lett.
282
65-72
2008
-
-
1
-
3
-
-
-
1
-
-
-
-
6
-
-
1
-
1
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692392
Frikha
3-D structure modelling of the ...
Staphylococcus simulans
FEMS Microbiol. Lett.
286
207-221
2008
-
-
-
-
-
-
-
-
-
2
-
-
-
12
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
692415
Mrdakovic
Partial characterization of a ...
Lymantria dispar
Folia Biol. (Krakow)
56
103-110
2008
-
-
-
-
-
-
7
1
-
1
-
-
-
11
-
-
-
-
-
17
-
-
4
-
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
1
-
-
-
-
-
-
-
17
-
-
4
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
692424
Chakraborty
An extra-cellular alkaline met ...
Bacillus licheniformis, Bacillus licheniformis MTCC 6824
Food Chem.
109
727-736
2008
1
-
-
-
-
-
12
6
-
2
1
-
2
12
-
-
1
-
-
-
-
-
12
1
1
1
-
1
-
1
1
1
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-
-
-
1
-
-
-
-
-
-
-
13
-
6
-
2
1
-
2
-
-
1
-
-
-
-
12
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
692714
Lumor
Esterification and hydrolytic ...
Diutina rugosa
J. Agric. Food Chem.
56
10396-10398
2008
-
-
-
-
-
1
-
-
-
-
-
-
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6
-
-
-
-
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4
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2
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1
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1
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4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692719
Chakraborty
Enrichment of eicosapentaenoic ...
Bacillus licheniformis, Bacillus licheniformis MTCC 6824
J. Agric. Food Chem.
56
1428-1433
2008
-
-
-
-
-
-
-
-
-
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1
-
-
13
-
-
1
-
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4
-
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2
-
1
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1
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1
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4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692905
Kasana
Isolation and identification o ...
Acinetobacter sp., Acinetobacter sp. CR9
J. Basic Microbiol.
48
207-212
2008
3
-
-
-
-
-
2
-
-
4
-
-
-
16
-
-
1
-
-
-
-
-
11
-
1
1
-
1
-
1
-
1
-
-
-
-
3
-
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-
-
-
-
2
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-
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4
-
-
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1
-
-
-
-
11
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
693072
Schweiger
The C-terminal region of human ...
Homo sapiens
J. Biol. Chem.
283
17211-17220
2008
1
-
1
-
4
-
-
-
3
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6
-
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-
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3
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1
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2
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1
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1
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4
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3
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3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693130
Chen
Identification and characteriz ...
Thermobifida fusca
J. Biol. Chem.
283
25854-25862
2008
-
-
2
-
2
-
-
-
-
-
2
-
-
2
-
-
2
-
-
-
-
-
4
-
2
2
2
2
-
2
2
-
-
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2
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-
2
-
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-
2
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2
-
-
-
-
4
-
2
2
2
-
2
2
-
-
-
-
-
-
-
-
693283
Sakai
Transesterification by lipase ...
Rhizopus oryzae
J. Biosci. Bioeng.
105
687-689
2008
-
-
-
-
-
1
-
-
-
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-
1
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-
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693476
Sanchez-Otero
Immobilization in the presence ...
Geobacillus thermoleovorans, Geobacillus thermoleovorans CCR11
J. Ind. Microbiol. Biotechnol.
35
1687-1693
2008
-
-
-
-
-
1
-
-
-
-
-
-
3
14
-
-
1
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-
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1
2
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1
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-
1
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1
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3
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1
-
-
-
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
693511
Imamura
A novel method for measuring h ...
Homo sapiens
J. Lipid Res.
49
1431-1437
2008
-
-
-
-
-
-
-
-
-
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-
-
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4
-
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1
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5
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1
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3
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1
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5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693692
Martin
-
Selective esterification of ph ...
Geobacillus thermocatenulatus
J. Mol. Catal. B
52-53
162-167
2008
1
-
-
-
-
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-
-
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1
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7
-
1
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1
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-
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-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693794
Edashige
Inhibitory effect of pectin fr ...
Sus scrofa
J. Nutr. Sci. Vitaminol.
54
409-415
2008
-
-
-
-
-
-
3
-
1
-
-
-
-
3
-
-
-
-
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2
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1
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1
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3
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1
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2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694108
Quintana-Castro
Gene cloning, expression, and ...
Geobacillus thermoleovorans, Geobacillus thermoleovorans CCR11
Mol. Biotechnol.
42
75-83
2008
-
-
1
-
-
-
-
-
-
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3
-
-
11
-
-
-
-
-
-
-
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6
-
2
2
2
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2
2
-
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1
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-
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3
-
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-
-
-
-
-
6
-
2
2
-
-
2
2
-
-
-
-
-
-
-
-
694531
Abada
Production and characterizatio ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus AB-1
Pak. J. Biol. Sci.
11
1100-1106
2008
-
-
-
-
-
-
-
-
-
-
-
-
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7
-
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1
-
-
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2
-
2
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1
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1
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1
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-
-
2
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
694532
Kammoun
Purification and characterizat ...
Testudo graeca nabeulensis
Pancreas
36
302-308
2008
-
-
-
-
-
-
5
-
-
-
1
-
-
3
-
-
1
-
-
1
-
-
2
-
2
1
-
1
-
1
-
1
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-
-
-
-
-
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-
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5
-
-
-
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1
-
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1
-
1
-
-
2
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
694702
Kram
Identification, cloning and ch ...
Jacaranda mimosifolia
Plant Mol. Biol.
68
173-183
2008
-
-
1
-
-
-
-
-
-
-
3
-
-
5
-
-
-
-
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2
-
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3
-
3
-
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-
-
-
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1
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-
1
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3
-
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-
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2
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
694786
Kobayashi
Constituents of Rhodiola rosea ...
Mus musculus
Planta Med.
74
1716-1719
2008
-
-
-
-
-
-
3
-
-
-
-
-
-
14
-
-
-
-
-
19
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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3
3
-
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-
-
-
-
-
-
-
-
-
19
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694844
Gaur
-
Purification and characterizat ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa PseA
Proc. Biochem.
43
1040-1046
2008
2
-
-
-
-
-
12
1
-
-
1
-
3
2
-
-
1
-
-
-
2
-
12
-
1
1
-
1
-
1
-
1
-
-
-
-
2
-
-
-
-
-
-
-
12
-
1
-
-
1
-
3
-
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1
-
-
2
-
12
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
694933
Zhao
-
Biochemical properties and pot ...
Serratia marcescens, Serratia marcescens ECU1010
Process Biochem.
43
626-633
2008
3
-
-
-
-
-
21
-
-
1
2
-
-
3
-
-
1
-
-
-
2
-
21
1
2
-
-
1
-
1
-
1
-
-
1
-
3
-
-
-
-
-
-
-
21
-
-
-
1
2
-
-
-
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1
-
-
2
-
21
1
-
-
1
-
1
-
1
1
-
-
-
-
-
-
694953
Kuwahara
Importance of the Ca2+-binding ...
Pseudomonas sp., Pseudomonas sp. MIS38
Protein Eng. Des. Sel.
21
737-744
2008
-
-
1
1
3
-
-
-
-
1
-
-
-
5
-
-
1
-
-
-
4
-
4
-
1
-
-
-
-
-
-
-
-
-
-
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-
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1
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1
3
-
-
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1
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-
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1
-
-
4
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694965
Chandrayan
Expression, purification, refo ...
Pyrococcus furiosus
Protein Expr. Purif.
59
327-333
2008
-
-
1
-
1
-
1
-
-
-
4
-
1
6
-
-
1
-
1
-
-
-
3
1
1
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
4
-
1
-
-
1
1
-
-
-
3
1
1
1
1
-
1
-
-
-
-
-
-
-
-
-
695169
Alatorre-Santamaria
Efficient access to enantiomer ...
Moesziomyces antarcticus
Tetrahedron Asymmetry
19
1714-1719
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695170
Sanfilippo
-
Lipase-catalyzed kinetic resol ...
Pseudomonas fluorescens
Tetrahedron Asymmetry
19
2171-2176
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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2
-
1
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-
-
-
-
-
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-
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-
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-
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-
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-
-
-
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-
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-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708593
Pinent
Differential transcriptional m ...
Mus musculus
Genomics
92
26-32
2008
-
-
-
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
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-
-
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-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
709226
Miyoshi
Adipose triglyceride lipase re ...
Mus musculus
J. Cell. Biochem.
105
1430-1436
2008
-
-
1
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
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-
1
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-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
709776
Deiuliis
Developmental, hormonal, and n ...
Sus scrofa
Lipids
43
215-225
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
709777
Rouhani
HDL composition regulates disp ...
Homo sapiens
Lipids
43
793-804
2008
2
-
-
-
-
-
1
-
2
-
-
-
-
3
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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1
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-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
742969
Rodrigues
-
Lipase-catalyzed ethanolysis ...
Thermomyces lanuginosus
J. Chem. Technol. Biotechnol.
83
849-854
2008
-
1
-
-
-
-
-
-
-
-
-
-
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1
-
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-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677300
Hasan
Purification and characterizat ...
Bacillus subtilis, Bacillus subtilis FH5
Acta Biol. Hung.
58
115-132
2007
-
-
-
-
-
-
10
1
-
6
2
-
-
7
-
-
1
-
-
-
-
-
4
1
1
1
1
7
-
1
1
8
-
-
-
-
-
-
-
-
-
-
-
-
10
-
1
-
6
2
-
-
-
-
1
-
-
-
-
4
1
1
1
7
-
1
1
8
-
-
-
-
-
-
-
677635
Lianghua
Purification and application o ...
Penicillium expansum, Penicillium expansum PED-03
Appl. Biochem. Biotechnol.
142
194-199
2007
-
1
-
-
-
-
-
-
1
-
1
-
-
10
-
-
1
-
-
1
1
-
4
1
-
2
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
1
-
4
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
677702
Salameh
Purification and characterizat ...
Thermosyntropha lipolytica
Appl. Environ. Microbiol.
73
7725-7731
2007
-
-
-
-
-
1
3
-
1
2
2
-
-
1
-
-
1
-
-
1
2
1
4
1
2
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
2
2
-
-
-
-
1
-
1
2
1
4
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
677772
Romero
Catalytic properties of myceli ...
Aspergillus niger
Appl. Microbiol. Biotechnol.
76
861-866
2007
1
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2
3
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1
1
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2
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1
3
1
1
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3
1
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1
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2
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1
1
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2
-
3
1
1
-
3
1
-
-
-
-
-
-
-
-
678010
Perez
-
Influence of the functional ac ...
Diutina rugosa
Biochem. Eng. J.
34
13-19
2007
-
-
-
-
1
-
-
5
-
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-
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1
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1
2
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2
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2
2
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2
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1
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5
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1
2
-
2
-
2
-
-
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2
-
-
-
-
-
-
-
-
-
678491
Cherif
Crab digestive lipase acting a ...
Homarus americanus
Biochimie
89
1012-1018
2007
-
-
-
-
-
-
1
-
-
-
1
-
-
5
-
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1
-
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2
1
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2
1
2
1
1
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1
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1
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1
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1
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2
1
-
2
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
678497
Zouari
Scorpion digestive lipase: a m ...
Scorpio maurus
Biochimie
89
403-409
2007
-
-
-
-
-
-
3
-
-
1
-
-
-
12
-
-
1
-
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2
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2
1
2
1
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-
-
1
-
-
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1
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-
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-
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3
-
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1
-
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1
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2
-
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2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
678851
Fernandez-Lorente
Improved catalytic properties ...
Moesziomyces antarcticus, Pseudomonas fluorescens
Biotechnol. Bioeng.
97
242-250
2007
3
-
-
-
2
-
2
-
-
-
-
-
-
2
-
-
-
-
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2
1
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3
-
1
2
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2
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3
-
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2
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2
-
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2
1
-
3
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
679192
Carballeira
Directed evolution and axial c ...
Pseudomonas aeruginosa
Chem. Commun. (Camb. )
2007
1913-1915
2007
-
-
-
-
16
-
-
-
-
-
-
-
-
6
-
-
-
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1
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2
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-
16
-
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1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679289
Yamada
New procedure for the measurem ...
Homo sapiens
Clin. Chim. Acta
383
85-90
2007
-
-
-
-
-
-
-
-
-
1
-
-
-
6
-
-
-
-
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9
1
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1
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1
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-
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1
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1
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1
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1
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9
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
679716
Ogino
Cloning and expression of gene ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa LST-03
Extremophiles
11
809-817
2007
-
-
1
-
-
-
-
-
1
-
-
-
-
10
-
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1
-
1
-
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2
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3
1
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1
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1
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1
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1
1
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-
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2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
679795
Berton
Role of the structural domains ...
Equus caballus
FEBS J.
274
6011-6023
2007
3
-
1
-
1
-
3
-
-
-
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4
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1
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5
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2
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3
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3
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1
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1
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3
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5
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679881
Hardeman
Metagenomic approach for the i ...
uncultured bacterium
FEMS Microbiol. Ecol.
59
524-534
2007
-
-
1
-
-
-
-
2
-
-
-
-
-
9
-
-
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1
-
-
-
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8
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3
1
1
1
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1
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1
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2
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-
-
-
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8
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
680972
Pilarek
Kinetic model of 1,3-specific ...
Moesziomyces antarcticus
J. Biotechnol.
127
736-744
2007
-
-
-
-
-
-
1
1
-
-
-
-
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4
-
-
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1
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1
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3
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1
1
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1
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1
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1
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3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
681046
Paula
-
Porcine pancreatic lipase immo ...
Sus scrofa
J. Chem. Technol. Biotechnol.
82
281-288
2007
-
1
-
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1
-
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2
-
-
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1
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-
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2
1
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1
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2
1
3
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1
2
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1
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1
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2
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2
1
-
1
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2
1
3
-
1
2
-
-
-
-
-
-
-
-
681253
Clarenbach
Variability in postheparin hep ...
Bos taurus
J. Investig. Med.
55
187-194
2007
-
-
-
-
-
-
-
3
1
-
1
1
-
3
-
1
1
-
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1
-
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4
1
1
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
3
1
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1
1
-
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1
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681263
Eydoux
Further biochemical characteri ...
Homo sapiens
J. Lipid Res.
48
1539-1549
2007
2
-
1
-
-
-
-
-
-
1
3
-
-
17
-
1
1
-
-
9
1
-
5
1
2
1
-
-
-
1
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
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1
3
-
-
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1
1
-
9
1
-
5
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
681355
Zhang
Cloning, expression, and chara ...
Psychrobacter sp.
J. Microbiol. Biotechnol.
17
604-610
2007
8
-
1
-
-
-
9
-
1
2
2
-
-
7
-
-
1
1
-
1
1
-
14
1
1
1
1
3
-
1
-
1
-
-
-
-
8
-
1
-
-
-
-
-
9
-
-
1
2
2
-
-
-
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1
-
1
1
-
14
1
1
1
3
-
1
-
1
-
-
-
-
-
-
-
681358
Lee
Molecular cloning and function ...
Serratia marcescens, Serratia marcescens ES-2
J. Microbiol. Biotechnol.
17
74-80
2007
1
-
1
-
-
-
-
-
1
1
3
-
-
13
-
-
1
-
-
-
2
-
21
1
2
1
1
-
-
1
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
3
-
-
-
-
1
-
-
2
-
21
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
681489
Yang
-
Cloning, expression and charac ...
Burkholderia cepacia
J. Mol. Catal. B
45
91-96
2007
-
-
2
-
-
-
-
-
-
-
1
-
6
2
-
-
1
2
-
-
-
-
2
1
2
2
2
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
1
-
-
-
-
2
1
2
2
2
-
2
-
-
-
-
-
-
-
-
-
681490
Bezbradica
-
The effect of substrate polari ...
Rhizomucor miehei, Sus scrofa
J. Mol. Catal. B
45
97-101
2007
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
2
-
3
2
-
2
-
5
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
2
-
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
681492
Yan
-
Gene cloning, overexpression a ...
Geotrichum candidum, Geotrichum candidum Y05
J. Mol. Catal. B
49
28-35
2007
-
-
2
-
-
-
-
-
-
-
1
-
-
3
-
1
1
1
-
-
2
-
16
1
6
2
-
1
-
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
2
-
16
1
2
-
1
-
2
-
2
-
-
-
-
-
-
-
681858
Diaz
Effect of nonionic surfactants ...
Rhizopus homothallicus
Mol. Biotechnol.
35
205-214
2007
3
-
-
-
-
-
1
-
-
-
1
-
-
6
-
-
1
-
-
1
2
-
1
-
3
1
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
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1
-
1
2
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
682607
Singh
-
Purification and characterizat ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa MTCC 5113
Process Biochem.
42
1063-1068
2007
1
-
-
-
-
-
4
1
1
-
2
-
-
2
-
-
1
-
-
-
1
-
4
1
1
1
-
-
-
1
-
3
-
-
1
-
1
-
-
-
-
-
-
-
4
-
1
1
-
2
-
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
3
1
-
-
-
-
-
-
682608
Soliman
-
Molecular cloning and characte ...
Geobacillus thermoleovorans, Geobacillus thermoleovorans YN
Process Biochem.
42
1090-1100
2007
2
-
1
-
-
-
3
1
-
4
2
-
-
2
-
1
1
-
-
-
1
-
19
1
4
1
-
1
-
1
-
2
-
-
-
-
2
-
1
-
-
-
-
-
3
-
1
-
4
2
-
-
-
1
1
-
-
1
-
19
1
1
-
1
-
1
-
2
-
-
-
-
-
-
-
682611
Yu
-
Purification and characterizat ...
Yarrowia lipolytica
Process Biochem.
42
384-391
2007
1
-
1
-
-
-
6
-
1
1
1
1
5
1
-
1
1
-
-
-
2
-
6
1
3
1
1
1
-
1
1
1
-
-
-
-
1
-
1
-
-
-
-
-
6
-
-
1
1
1
1
5
-
1
1
-
-
2
-
6
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
682616
Chen
-
Purification and properties of ...
Bacillus cereus, Bacillus cereus C71
Process Biochem.
42
988-994
2007
4
1
-
-
-
-
8
-
-
1
1
-
-
2
-
-
1
-
-
-
1
-
6
1
1
1
-
1
-
1
1
1
-
-
-
-
4
1
-
-
-
-
-
-
8
-
-
-
1
1
-
-
-
-
1
-
-
1
-
6
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
682667
Yu
High-level expression of extra ...
Yarrowia lipolytica
Protein Expr. Purif.
53
255-263
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
14
-
1
1
-
-
-
1
-
8
-
3
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
8
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
682678
Mosbah
Involvement of Gly 311 residue ...
Staphylococcus xylosus
Protein Expr. Purif.
55
31-39
2007
-
-
1
-
4
-
-
8
-
-
1
-
-
11
-
-
1
1
-
-
8
-
2
1
2
1
1
1
8
3
1
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
8
-
-
1
-
-
-
-
1
-
-
8
-
2
1
1
1
1
8
3
1
-
-
-
-
-
-
-
-
664719
Kershaw
Adipose triglyceride lipase: f ...
Mus musculus
Diabetes
55
148-157
2006
1
-
1
-
1
-
1
-
-
-
-
1
-
5
-
-
-
-
-
3
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665288
Chang
Codon optimization of Candida ...
Diutina rugosa
J. Agric. Food Chem.
54
815-822
2006
-
1
1
-
1
-
3
-
-
-
1
-
-
13
-
-
1
-
-
-
2
-
7
1
3
1
-
1
-
1
-
1
-
-
-
-
-
1
1
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
1
-
-
2
-
7
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
665729
Deb
A novel lipase belonging to th ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
281
3866-3875
2006
2
-
1
-
1
-
9
1
-
2
1
-
-
9
-
-
1
-
-
-
2
2
4
1
4
1
-
1
-
1
-
-
-
-
1
-
2
-
1
-
-
1
-
-
9
-
1
-
2
1
-
-
-
-
1
-
-
2
2
4
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
665746
Freie
Val-407 and Ile-408 in the bet ...
Homo sapiens
J. Biol. Chem.
281
7793-7800
2006
-
-
1
-
6
-
-
1
-
-
-
-
-
4
-
-
1
-
-
2
1
-
4
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
666789
Ng
-
Investigation of lipases from ...
Carica papaya
Process Biochem.
41
540-546
2006
-
-
-
-
-
-
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1
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-
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-
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1
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1
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4
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8
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1
2
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1
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1
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1
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4
-
8
-
2
-
-
-
1
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-
-
-
-
-
-
-
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677728
Pfeffer
High yield expression of lipas ...
Moesziomyces antarcticus
Appl. Microbiol. Biotechnol.
72
931-938
2006
-
-
1
-
-
-
-
1
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1
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12
-
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1
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1
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2
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2
1
1
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1
1
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1
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1
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-
1
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1
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1
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
677737
Luo
A novel psychrophilic lipase f ...
Pseudomonas fluorescens, Pseudomonas fluorescens B68
Appl. Microbiol. Biotechnol.
73
349-355
2006
-
1
1
-
-
-
-
-
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10
-
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1
-
1
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4
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3
1
1
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1
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1
1
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1
1
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-
-
4
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
677808
Fendri
Biochemical characterization, ...
Gallus gallus
Arch. Biochem. Biophys.
451
149-159
2006
3
-
1
-
-
-
2
-
-
-
2
-
-
6
-
1
1
-
-
5
1
-
4
2
2
1
-
-
-
1
-
-
-
-
-
-
3
-
1
-
-
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2
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2
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1
1
-
5
1
-
4
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
677840
Patel
Adipokinetic hormone-induced m ...
Manduca sexta
Arch. Insect Biochem. Physiol.
63
73-81
2006
1
-
-
-
-
-
-
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3
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1
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9
-
1
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3
1
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3
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1
1
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1
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1
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3
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1
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1
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3
1
-
3
-
1
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1
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-
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678397
Aloulou
Exploring the specific feature ...
Yarrowia lipolytica
Biochim. Biophys. Acta
1761
995-1013
2006
2
1
1
-
1
-
1
-
-
-
2
-
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1
-
1
1
-
-
-
4
-
5
1
3
1
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2
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1
-
3
-
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2
1
1
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1
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1
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2
-
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1
1
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-
4
-
5
1
1
-
2
-
1
-
3
-
-
-
-
-
-
-
679311
Zouari
Scorpion digestive lipase: kin ...
Scorpio maurus
Colloids Surf. B Biointerfaces
49
8-14
2006
2
-
-
-
-
1
1
1
-
-
1
-
-
1
-
-
1
-
-
1
-
-
4
1
2
1
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1
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2
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1
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1
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1
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1
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1
-
1
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
679373
Joshi
Purification of alkaline lipas ...
Tsukamurella paurometabola, Tsukamurella paurometabola MTCC 6841
Curr. Microbiol.
52
354-358
2006
-
-
-
-
-
-
-
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1
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10
-
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1
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1
1
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1
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1
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-
1
-
-
-
-
-
-
-
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-
-
-
-
-
-
679894
Salah
Biochemical and molecular char ...
Rhizopus oryzae, Rhizopus oryzae WPG
FEMS Microbiol. Lett.
260
241-248
2006
1
-
1
-
-
-
1
-
1
-
1
-
-
16
-
-
1
-
-
13
2
-
6
1
4
1
-
-
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1
-
1
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-
-
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1
-
1
-
-
-
-
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1
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1
-
1
-
-
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1
-
13
2
-
6
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
680279
Chang
Efficient production of active ...
Diutina rugosa
J. Agric. Food Chem.
54
5831-5838
2006
2
-
1
-
1
-
8
-
-
2
-
-
-
11
-
-
1
-
-
-
2
-
1
-
3
1
1
1
-
1
1
1
-
-
-
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2
-
1
-
-
1
-
-
8
-
-
-
2
-
-
-
-
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1
-
-
2
-
1
-
1
1
1
-
1
1
1
-
-
-
-
-
-
-
680948
Karadzic
Purification and characterizat ...
Pseudomonas aeruginosa
J. Biosci. Bioeng.
102
82-89
2006
8
1
-
-
-
-
10
-
1
-
1
1
9
6
-
-
1
-
-
4
6
-
9
1
1
1
1
3
-
1
1
1
-
-
-
-
8
1
-
-
-
-
-
-
10
-
-
1
-
1
1
9
-
-
1
-
4
6
-
9
1
1
1
3
-
1
1
1
-
-
-
-
-
-
-
681258
Griffon
Substrate specificity of lipop ...
Homo sapiens
J. Lipid Res.
47
1803-1811
2006
-
-
-
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3
-
-
-
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1
-
1
-
-
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-
1
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3
-
3
-
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3
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-
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1
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-
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-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681482
Bae
-
Enantioselective properties of ...
Serratia marcescens, Serratia marcescens ES-2
J. Mol. Catal. B
40
24-29
2006
5
-
1
-
-
-
6
-
1
3
1
-
-
2
-
-
1
-
-
-
1
-
2
1
2
1
-
-
-
1
-
-
-
-
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5
-
1
-
-
-
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6
-
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1
3
1
-
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1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
681485
Niu
-
Improved thermostability and t ...
Rhizopus oryzae
J. Mol. Catal. B
43
33-39
2006
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
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1
1
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2
-
1
-
2
1
2
1
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1
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1
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-
4
-
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-
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-
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1
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-
2
-
1
-
1
2
1
-
1
-
-
-
-
-
-
-
-
-
682643
Kanwar
Purification and properties of ...
Bacillus coagulans, Bacillus coagulans MTCC-6375
Protein Expr. Purif.
46
421-428
2006
-
-
-
-
-
-
5
1
1
6
1
-
-
5
-
-
1
-
-
-
1
-
4
1
1
1
-
4
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
1
6
1
-
-
-
-
1
-
-
1
-
4
1
1
-
4
-
1
-
1
-
-
-
-
-
-
-
682645
Song
Expression and purification of ...
Yarrowia lipolytica, Yarrowia lipolytica AS 2.1216
Protein Expr. Purif.
47
393-397
2006
-
-
2
-
-
-
-
-
-
-
2
-
2
15
-
2
2
-
-
-
2
-
6
2
4
2
-
2
-
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
2
-
2
2
-
-
2
-
6
2
2
-
2
-
2
-
2
-
-
-
-
-
-
-
682646
Mosbah
Expression, purification, and ...
Staphylococcus xylosus
Protein Expr. Purif.
47
516-523
2006
1
-
1
-
1
-
2
4
1
1
1
-
-
11
-
-
1
-
-
-
3
-
4
1
2
1
-
-
3
1
-
-
-
-
-
-
1
-
1
-
-
1
-
-
2
-
4
1
1
1
-
-
-
-
1
-
-
3
-
4
1
1
-
-
3
1
-
-
-
-
-
-
-
-
-
682990
Petersen
Purification and characterizat ...
Clostridium tetanomorphum, Clostridium tetanomorphum NCTC 543
World J. Microbiol. Biotechnol.
22
431-435
2006
1
-
-
-
-
-
-
-
1
-
2
-
-
2
-
-
1
-
-
1
1
-
13
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
1
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663730
Gupta
Single-step purification of li ...
Burkholderia multivorans, Burkholderia multivorans RG2
Appl. Microbiol. Biotechnol.
67
648-653
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
1
-
-
-
1
-
2
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
664060
Poulsen
Quantization of pH: evidence f ...
Fusarium solani, Rhizomucor miehei, Thermomyces lanuginosus, Burkholderia cepacia
Biochemistry
44
11574-11580
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
13
-
-
1
4
-
-
2
-
4
-
9
1
-
-
-
4
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
4
-
1
-
-
-
4
4
-
-
-
-
-
-
-
-
664191
Zouari
Purification and characterizat ...
Scorpio maurus
Biochim. Biophys. Acta
1726
67-74
2005
-
-
-
-
-
-
-
2
-
1
1
-
-
9
-
1
1
1
-
3
2
-
3
1
3
1
-
1
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
1
1
-
3
2
-
3
1
1
-
1
1
1
1
1
-
-
-
-
-
-
-
664788
Ben Bacha
-
Purification and biochemical c ...
Struthio camelus
Enzyme Microb. Technol.
37
309-317
2005
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
1
1
-
-
1
1
-
4
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
1
-
1
1
-
4
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
664795
Castro-Ochoa
-
Screening, purification and ch ...
Geobacillus thermoleovorans, Geobacillus thermoleovorans CCR11
Enzyme Microb. Technol.
37
648-654
2005
-
-
-
-
-
-
5
-
-
2
-
2
5
2
-
-
1
-
-
1
-
-
4
-
1
1
-
3
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
5
-
-
-
2
-
2
5
-
-
1
-
1
-
-
4
-
1
-
3
-
1
-
1
1
-
-
-
-
-
-
664866
Carvalho
Trichomonas vaginalis: identif ...
Trichomonas vaginalis
Exp. Parasitol.
111
260-263
2005
-
-
1
-
-
-
-
-
-
1
1
1
-
6
-
-
-
1
-
-
-
-
1
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664964
El-Kouhen
Identification and characteriz ...
Arabidopsis thaliana
FEBS Lett.
579
6067-6073
2005
-
-
1
-
1
-
-
-
-
-
-
2
-
10
-
1
1
-
-
3
1
-
7
1
2
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
1
-
3
1
-
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
664965
Sayari
N-terminal peptide of Rhizopus ...
Rhizopus oryzae
FEBS Lett.
579
976-982
2005
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
1
1
1
-
1
-
-
5
1
5
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
665123
Kobayashi
Relationship of lipoprotein li ...
Homo sapiens
Horm. Metab. Res.
37
505-509
2005
-
-
-
-
-
-
-
-
-
1
-
1
-
4
-
-
-
-
-
7
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
7
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665175
Sakinc
The surface-associated protein ...
Staphylococcus saprophyticus
Infect. Immun.
73
6419-6428
2005
-
-
1
-
1
-
-
-
1
1
-
1
-
10
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665194
Auerswald
Activation of triacylglycerol ...
Locusta migratoria, Periplaneta americana, Pachnoda sinuata flaviventris
Insect Biochem. Mol. Biol.
35
461-470
2005
3
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
5
3
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665672
Athenstaedt
Tgl4p and Tgl5p, two triacylgl ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
J. Biol. Chem.
280
37301-37309
2005
-
-
1
-
-
-
-
-
2
1
-
6
-
76
-
-
1
-
-
-
-
-
13
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
6
-
-
-
1
-
-
-
-
13
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
665778
Fucinos
Identification of extracellula ...
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
J. Biotechnol.
117
233-241
2005
-
-
-
-
-
-
-
-
1
-
1
-
1
24
-
-
1
-
-
-
1
-
2
1
2
1
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
1
-
2
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
666297
Gilham
Triacylglycerol hydrolase is l ...
Homo sapiens
Mol. Biol. Cell
16
984-996
2005
-
-
1
-
2
-
-
-
1
-
-
1
-
5
-
1
-
-
-
2
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
1
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666695
Sagiroglu
Sunflower seed lipase: extract ...
Helianthus annuus
Prep. Biochem. Biotechnol.
35
37-51
2005
-
-
-
-
-
-
-
1
-
-
1
-
-
9
-
-
1
-
-
3
1
1
1
1
1
1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
3
1
1
1
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
666821
Kim
Purification, refolding, and c ...
Pseudomonas fluorescens, Pseudomonas fluorescens SIK W1
Protein Expr. Purif.
39
124-129
2005
-
-
1
-
-
1
-
-
2
1
-
2
-
11
-
-
1
-
1
-
1
-
2
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
2
-
-
-
1
1
-
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
666825
Quyen
High-level heterologous expres ...
Ralstonia sp., Ralstonia sp. M1
Protein Expr. Purif.
39
97-106
2005
16
-
1
-
-
-
17
-
-
5
1
7
-
6
-
-
1
-
1
-
-
-
12
1
2
1
1
1
-
1
1
1
-
-
-
-
16
-
1
-
-
-
-
-
17
-
-
-
5
1
7
-
-
-
1
1
-
-
-
12
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
666830
Kumar
Production, purification, and ...
Bacillus coagulans, Bacillus coagulans BTS-3
Protein Expr. Purif.
41
38-44
2005
-
-
-
-
-
-
4
-
1
5
1
-
-
10
-
-
1
-
-
2
2
-
11
1
2
1
-
3
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
5
1
-
-
-
-
1
-
2
2
-
11
1
1
-
3
-
1
1
1
-
-
-
-
-
-
-
666831
Li
Characterization of thermostab ...
Geobacillus stearothermophilus
Protein Expr. Purif.
42
153-159
2005
-
-
1
-
-
-
4
-
-
5
-
-
-
9
-
-
1
-
-
-
-
-
-
-
2
2
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
667017
Böer
An extracellular lipase from t ...
Blastobotrys adeninivorans
Yeast
22
523-535
2005
-
-
1
-
-
-
3
-
1
4
3
-
1
13
-
1
1
-
-
-
1
-
4
1
2
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
4
3
-
1
-
1
1
-
-
1
-
4
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
680528
Sekhon
Properties of a thermostable e ...
Priestia megaterium, Priestia megaterium AKG-1
J. Basic Microbiol.
45
147-154
2005
9
-
-
-
-
-
7
-
1
1
1
-
-
7
-
-
1
-
-
-
1
-
6
1
1
1
1
2
-
1
1
-
-
-
-
-
9
-
-
-
-
-
-
-
7
-
-
1
1
1
-
-
-
-
1
-
-
1
-
6
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
682482
Cherukuvada
Evidence of a double-lid movem ...
Pseudomonas aeruginosa
PLoS Comput. Biol.
1
e28
2005
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682488
Saeed
Purification and characterizat ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa Ps-x
Pol. J. Microbiol.
54
233-240
2005
1
-
-
-
-
-
3
-
1
5
2
-
-
4
-
-
1
-
-
-
2
-
2
1
3
2
1
2
-
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
1
5
2
-
-
-
-
1
-
-
2
-
2
1
2
1
2
-
2
1
-
-
-
-
-
-
-
-
682631
Fujii
Directed evolution of Pseudomo ...
Pseudomonas aeruginosa
Protein Eng. Des. Sel.
18
93-101
2005
-
-
1
-
8
-
-
-
-
-
-
-
-
6
-
-
-
1
-
-
1
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
649346
Kapranchikov
-
Purification and characterizat ...
Triticum aestivum
Appl. Biochem. Microbiol.
40
84-88
2004
1
-
-
-
-
-
2
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
1
-
1
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
-
1
1
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
651516
Fernandez
Purification and characterizat ...
Micrococcus sp., Micrococcus sp. INIA 528
Int. Dairy J.
14
135-142
2004
-
-
-
-
-
-
12
4
1
1
1
-
-
2
-
-
1
-
-
-
1
-
17
1
1
1
-
3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
4
1
1
1
-
-
-
-
1
-
-
1
-
17
1
1
-
3
-
1
1
-
-
-
-
-
-
-
-
663726
Kaieda
Enantioselective transesterifi ...
Aspergillus oryzae
Appl. Microbiol. Biotechnol.
65
301-305
2004
-
1
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663907
Lima
-
Activity and stability of a cr ...
Penicillium aurantiogriseum
Biochem. Eng. J.
18
65-71
2004
1
-
-
-
-
-
6
-
-
8
-
-
9
1
-
-
-
-
-
-
6
-
7
-
-
1
1
5
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
-
8
-
-
9
-
-
-
-
-
6
-
7
-
1
1
5
-
1
-
1
-
-
-
-
-
-
-
664171
Haiker
Rapid exchange of pancreatic l ...
Homo sapiens, Sus scrofa
Biochim. Biophys. Acta
1682
72-79
2004
-
-
-
-
1
-
1
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
5
-
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
5
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
664172
Tsuzuki
Inhibitory effect of lysophosp ...
Sus scrofa
Biochim. Biophys. Acta
1684
1-7
2004
-
-
-
-
-
-
1
5
-
-
-
-
-
1
-
-
-
-
-
2
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
-
2
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
664561
Dolinsky
Triacylglycerol hydrolase: rol ...
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Cell. Mol. Life Sci.
61
1633-1651
2004
1
-
2
-
1
-
-
-
15
-
-
5
-
16
-
4
3
3
-
24
-
-
5
2
20
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
15
-
-
5
-
-
4
3
-
24
-
-
5
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664645
Fahmy
-
Esterase and lipase in camel t ...
Hyalomma dromedarii
Comp. Biochem. Physiol. B
137B
159-168
2004
-
-
-
-
-
-
11
1
-
1
-
-
-
1
-
-
1
-
-
1
1
-
1
-
1
1
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
11
2
1
-
1
-
-
-
-
-
1
-
1
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
664934
Matsui
A tomato lipase homologous to ...
Solanum lycopersicum
FEBS Lett.
569
195-200
2004
-
-
1
-
-
-
-
-
2
-
1
1
-
9
-
-
1
-
-
7
-
-
6
1
2
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
1
-
7
-
-
6
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
665266
Suzuki
Purification and characterizat ...
Fagopyrum esculentum
J. Agric. Food Chem.
52
7407-7411
2004
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
1
-
-
3
4
-
8
1
2
2
-
1
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
3
4
-
8
1
2
-
1
-
3
2
-
-
-
-
-
-
-
-
665576
Eastmond
Cloning and characterization o ...
Ricinus communis
J. Biol. Chem.
279
45540-45545
2004
1
-
1
-
-
-
4
-
2
1
1
1
-
12
-
-
-
1
-
5
7
-
11
1
2
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
-
2
1
1
1
-
-
-
-
-
5
7
-
11
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
665586
Jenkins
Identification, cloning, expre ...
Homo sapiens
J. Biol. Chem.
279
48968-48975
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
666216
Huang
Purification and characterizat ...
Geotrichum marinum
Lipids
39
251-257
2004
2
-
-
-
-
-
9
1
1
4
1
-
-
6
-
-
1
-
-
-
1
-
5
1
1
1
1
2
-
1
1
-
-
-
1
-
2
-
-
-
-
-
-
-
9
-
1
1
4
1
-
-
-
-
1
-
-
1
-
5
1
1
1
2
-
1
1
-
1
-
-
-
-
-
-
666217
Akoh
Protein engineering and applic ...
Diutina rugosa
Lipids
39
513-526
2004
-
3
1
-
3
-
-
-
-
-
-
3
-
12
-
1
1
1
-
3
-
-
5
1
2
-
-
-
-
-
-
-
-
-
-
-
-
3
1
-
-
3
-
-
-
-
-
-
-
-
3
-
-
1
1
-
3
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666368
Ogino
Cloning, expression and charac ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa LST-03
Mol. Genet. Genomics
271
189-196
2004
-
-
1
-
-
1
-
-
-
-
-
-
16
11
-
-
1
-
-
-
-
1
4
-
1
1
1
2
-
1
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
16
-
-
1
-
-
-
1
4
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-
728732
Schlieben
Expression, purification, and ...
Geobacillus thermocatenulatus
Protein Expr. Purif.
34
103-110
2004
-
-
1
1
-
-
-
-
-
-
1
-
1
9
-
-
1
-
-
-
-
-
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
650524
Jinwal
Purification and characterizat ...
Pseudomonas mendocina, Pseudomonas mendocina PK-12CS
Bioorg. Med. Chem.
11
1041-1046
2003
-
1
-
-
-
-
9
-
1
2
1
6
1
8
-
-
1
-
-
-
1
-
11
1
2
1
-
5
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
9
-
-
1
2
1
6
1
-
-
1
-
-
1
-
11
1
1
-
5
-
1
1
-
-
-
-
-
-
-
-
650697
Ueji
Chemical modification of lipas ...
Diutina rugosa
Biotechnol. Lett.
25
83-87
2003
-
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
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1
-
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-
-
-
-
-
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-
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-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
650899
Miled
-
Importance of the lid and cap ...
Homo sapiens
Comp. Biochem. Physiol. B
136B
131-138
2003
-
-
1
-
1
-
-
-
1
-
3
-
-
1
-
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1
1
-
1
3
-
1
2
2
-
-
-
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1
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-
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1
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1
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1
-
3
-
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1
-
1
3
-
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
651027
Maruyama
-
Can lipases hydrolyze a peptid ...
Bacillus subtilis, Diutina rugosa, Fusarium solani, Homo sapiens, Thermomyces lanuginosus, Rhizomucor miehei, Rhizopus oryzae, Rhizopus japonicus, Sus scrofa, Chromobacterium viscosum, Alcaligenes ssp., Bacillus subtilis 168 BsL
Enzyme Microb. Technol.
32
655-657
2003
-
-
1
-
-
-
-
-
1
-
-
-
-
12
-
-
2
11
-
3
-
-
26
-
11
11
-
-
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
2
-
3
-
-
26
-
11
-
-
-
11
-
-
-
-
-
-
-
-
-
652407
Athenstaedt
YMR313c/TGL3 encodes a novel t ...
Saccharomyces cerevisiae
J. Biol. Chem.
278
23317-23323
2003
-
-
1
-
1
-
-
-
1
-
-
1
-
6
-
-
1
-
-
-
2
-
1
-
1
1
-
-
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1
-
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-
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1
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1
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1
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1
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1
-
-
2
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
652591
Kojima
Purification and characterizat ...
Pseudomonas fluorescens, Pseudomonas fluorescens HU380
J. Biosci. Bioeng.
96
219-226
2003
-
1
-
-
-
-
-
-
-
2
1
-
-
8
-
-
1
-
-
-
1
-
30
1
1
1
-
1
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
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1
-
-
1
-
30
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
652764
An
-
Cloning, expression in Escheri ...
Pseudomonas sp., Pseudomonas sp. SW-3
J. Microbiol.
41
95-101
2003
-
-
1
-
-
-
4
-
-
2
1
-
-
2
-
-
1
-
1
-
1
-
11
1
2
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
2
1
-
-
-
-
1
1
-
1
-
11
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
652958
Kambourova
-
Purification and properties of ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus MC 7
J. Mol. Catal. B
22
307-313
2003
1
1
-
-
-
-
13
1
1
1
1
-
11
2
-
-
1
-
-
-
1
-
19
1
1
1
1
1
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
13
-
1
1
1
1
-
11
-
-
1
-
-
1
-
19
1
1
1
1
-
1
-
-
-
-
-
-
-
-
-
653693
Saxena
-
Purification and characterizat ...
Aspergillus carneus
Process Biochem.
39
239-247
2003
5
-
-
-
-
1
7
1
-
3
1
-
4
1
-
1
1
1
-
-
1
1
7
1
1
1
1
2
-
1
-
-
-
-
1
-
5
-
-
-
-
-
1
-
7
-
1
-
3
1
-
4
-
1
1
-
-
1
1
7
1
1
1
2
-
1
-
-
1
-
-
-
-
-
-
653758
Quyen
High-level expression of a lip ...
Geobacillus thermocatenulatus, Geobacillus thermocatenulatus BTL2
Protein Expr. Purif.
28
102-110
2003
-
-
1
-
-
-
8
-
-
-
1
-
3
16
-
-
1
-
-
-
1
-
15
1
1
2
1
3
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
-
-
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1
-
3
-
-
1
-
-
1
-
15
1
2
1
3
-
2
1
-
-
-
-
-
-
-
-
650367
Kim
Expression and characterizatio ...
Bacillus pumilus, Bacillus pumilus B26
Biochim. Biophys. Acta
1583
205-212
2002
-
-
1
-
-
-
-
-
1
1
-
-
-
7
-
-
1
-
-
-
1
-
10
-
2
1
-
2
-
1
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
1
1
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-
-
-
-
1
-
-
1
-
10
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
651022
van Heerden
-
Biochemical characterization a ...
Aspergillus niger, Aspergillus niger Tiegh
Enzyme Microb. Technol.
30
902-909
2002
-
-
-
-
-
1
-
1
1
-
1
-
-
2
-
-
1
-
-
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1
-
12
1
1
1
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-
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1
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1
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1
1
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1
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1
-
-
1
-
12
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
651025
Sunna
-
Biochemical characterization o ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) Tp10A.1
Enzyme Microb. Technol.
31
472-476
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
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1
1
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-
-
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16
-
1
1
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1
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1
-
-
-
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-
1
-
-
-
-
16
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
651120
Neugnot
The lipase/acyltransferase fro ...
Candida parapsilosis
Eur. J. Biochem.
269
1734-1745
2002
-
-
1
-
-
-
-
-
-
-
1
-
-
12
-
1
1
-
-
-
1
-
1
1
3
2
-
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-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
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-
1
1
-
-
1
-
1
1
2
-
-
-
1
-
-
2
-
-
-
-
-
-
651141
Snellman
Purification and properties of ...
Acinetobacter sp., Acinetobacter sp. RAG-1
Eur. J. Biochem.
269
5771-5779
2002
6
-
-
-
-
1
6
-
1
-
1
-
5
7
-
-
1
-
-
-
1
-
16
1
2
1
1
1
-
1
1
-
-
-
1
-
6
-
-
-
-
-
1
-
6
-
-
1
-
1
-
5
-
-
1
-
-
1
-
16
1
1
1
1
-
1
1
-
1
-
-
-
-
-
-
653692
Sharma
-
Purification and characterizat ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) RSJ-1
Process Biochem.
37
1075-1084
2002
1
1
-
-
-
1
15
1
-
3
1
-
-
2
1
-
1
-
-
-
1
1
4
1
1
1
-
6
-
1
1
-
-
-
-
-
1
1
-
-
-
-
1
-
15
-
1
-
3
1
-
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1
-
1
-
-
1
1
4
1
1
-
6
-
1
1
-
-
-
-
-
-
-
-
653745
Alam
Heterologous expression, purif ...
Homo sapiens
Protein Expr. Purif.
24
33-42
2002
-
-
1
-
-
-
-
-
-
-
-
2
-
8
-
1
1
-
-
1
-
-
3
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
1
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
664815
Alquati
The cold-active lipase of Pseu ...
Pseudomonas fragi, Pseudomonas fragi IFO 3458
Eur. J. Biochem.
269
3321-3328
2002
-
-
1
-
-
-
-
-
-
1
-
-
-
15
-
-
1
-
-
-
-
-
13
1
2
1
1
3
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
13
1
1
1
3
-
1
-
-
-
-
-
-
-
-
-
679885
Ruiz
Analysis of Bacillus megateriu ...
Priestia megaterium, Priestia megaterium ATCC 9885
FEMS Microbiol. Lett.
217
263-267
2002
1
-
1
-
-
-
7
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
16
1
2
1
-
2
-
1
1
1
-
-
3
-
1
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
1
-
-
1
-
-
16
1
1
-
2
-
1
1
1
3
-
-
-
-
-
-
650348
van Kampen
Cloning, purification and char ...
Staphylococcus warneri, Staphylococcus warneri 863
Biochim. Biophys. Acta
1544
229-241
2001
-
-
1
-
-
-
1
-
1
1
1
-
-
11
-
1
1
-
-
-
4
-
11
1
2
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
1
1
-
-
-
1
1
-
-
4
-
11
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
650429
Sayari
Biochemical and molecular char ...
Staphylococcus simulans
Biochimie
83
863-871
2001
-
-
1
-
-
-
1
-
1
1
2
-
-
15
-
-
1
-
-
1
1
-
4
1
2
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
1
2
-
-
-
-
1
-
1
1
-
4
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
650654
Sharma
Production, purification, char ...
Acinetobacter calcoaceticus, Aspergillus niger, Aspergillus oryzae, Geobacillus stearothermophilus, Bacillus sp. (in: Bacteria), Burkholderia cepacia, Burkholderia sp., Moesziomyces antarcticus, Diutina rugosa, Rhizomucor miehei, Penicillium roqueforti, Hyphopichia burtonii, Proteus vulgaris, Pseudomonas sp., Pseudomonas aeruginosa, Pseudomonas alcaligenes, Pseudomonas oleovorans, Rhizopus oryzae, Rhodotorula glutinis, Staphylococcus epidermidis, Penicillium wortmanii, Penicillium roqueforti IAM7268, Bacillus sp. (in: Bacteria) J33, Acinetobacter calcoaceticus BD 413, Geobacillus stearothermophilus L1, Pseudomonas sp. KM1-56
Biotechnol. Adv.
19
627-662
2001
7
21
6
-
2
-
24
3
8
18
15
-
5
30
1
1
10
2
-
8
1
-
56
10
80
10
1
11
-
12
1
-
-
-
4
-
7
21
6
-
-
2
-
-
24
-
3
8
18
15
-
5
1
1
10
-
8
1
-
56
10
10
1
11
-
12
1
-
4
-
-
-
-
-
-
650688
Oh
-
Properties of recombinant Rhiz ...
Rhizomucor miehei
Biotechnol. Lett.
23
563-568
2001
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651018
Lee
-
Purification and characterizat ...
Geobacillus thermoleovorans, Geobacillus thermoleovorans ID-1
Enzyme Microb. Technol.
29
363-371
2001
5
-
1
-
-
-
18
2
-
-
1
2
1
2
-
-
1
-
-
-
2
-
18
1
1
2
-
2
-
2
-
-
-
-
-
-
5
-
1
-
-
-
-
-
18
-
2
-
-
1
2
1
-
-
1
-
-
2
-
18
1
2
-
2
-
2
-
-
-
-
-
-
-
-
-
651019
Lescic
-
Substrate specificity and effe ...
Streptomyces rimosus
Enzyme Microb. Technol.
29
548-553
2001
6
-
-
-
-
-
-
-
1
-
-
-
7
1
-
-
-
-
-
-
2
-
16
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
-
-
7
-
-
-
-
-
2
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651285
Pernas
Influence of the conformationa ...
Diutina rugosa
FEBS Lett.
501
87-91
2001
-
-
-
-
-
-
1
1
-
-
4
-
-
4
-
-
-
-
-
-
3
-
1
3
1
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
3
-
1
3
1
-
-
-
1
-
-
1
-
-
-
-
-
-
653102
Ruiz
Purification and characterizat ...
Penicillium candidum
Lipids
36
283-289
2001
6
-
-
-
-
-
5
-
1
-
1
-
-
9
-
-
1
-
-
-
1
-
16
2
1
1
-
1
-
1
-
-
-
-
1
-
6
-
-
-
-
-
-
-
5
-
-
1
-
1
-
-
-
-
1
-
-
1
-
16
2
1
-
1
-
1
-
-
1
-
-
-
-
-
-
653521
Bhardwaj
Identification, purification, ...
Oryza sativa
Plant Physiol.
127
1728-1738
2001
2
-
-
-
-
-
9
2
-
-
1
-
-
6
-
1
1
-
-
1
2
-
5
-
1
1
-
1
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
9
-
2
-
-
1
-
-
-
1
1
-
1
2
-
5
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
653738
Sinchaikul
Optimization of a thermostable ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus P1
Protein Expr. Purif.
22
388-398
2001
-
-
1
-
-
1
17
-
-
-
-
2
-
9
-
-
1
1
-
-
1
-
8
1
1
1
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
17
-
-
-
-
-
2
-
-
-
1
-
-
1
-
8
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
649985
Van Bennekum
Hydrolysis of retinyl esters b ...
Mus musculus, Rattus norvegicus
Biochemistry
39
4900-4906
2000
4
-
-
-
-
-
4
-
-
-
-
4
-
13
-
-
2
-
-
2
-
-
4
-
4
2
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
-
2
-
2
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
650306
Amada
Overproduction in Escherichia ...
Pseudomonas sp., Pseudomonas sp. MIS38
Biochim. Biophys. Acta
1478
201-210
2000
-
-
1
-
1
-
2
-
-
1
2
-
-
12
-
-
1
1
1
-
13
-
18
1
2
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
1
-
-
2
-
-
-
1
2
-
-
-
-
1
1
-
13
-
18
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
650618
Kim
Thermostable lipase of Bacillu ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus L1
Biosci. Biotechnol. Biochem.
64
280-286
2000
-
-
1
-
-
2
-
-
-
1
1
-
-
13
-
-
1
-
-
-
1
-
2
-
1
1
-
3
-
-
-
-
-
-
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
2
-
1
-
3
-
-
-
-
1
-
-
-
-
-
-
650660
Arreguin-Espinosa
Purification and properties of ...
Cephaloleia presignis
Biotechnol. Appl. Biochem.
31
239-244
2000
1
-
-
-
-
-
7
-
1
1
2
-
-
6
-
1
1
-
-
2
1
-
20
1
1
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
7
-
-
1
1
2
-
-
-
1
1
-
2
1
-
20
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
650684
Rathi
-
A hyper-thermostable, alkaline ...
Pseudomonas sp.
Biotechnol. Lett.
22
495-498
2000
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-
-
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-