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Literature summary for 3.1.1.29 extracted from

  • Taylor-Creel, K.; Hames, M.C.; Holloway, W.B.; McFeeters, H.; McFeeters, R.L.
    Expression, purification, and solubility optimization of peptidyl-tRNA hydrolase 1 from Bacillus cereus (2014), Protein Expr. Purif., 95, 259-264.
    View publication on PubMed
Search for more literature for 3.1.1.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 bulk peptidyl-tRNA at pH 5.0 and 25°C Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-substituted aminoacyl-tRNA + H2O Bacillus cereus
-
 N-substituted amino acid + tRNA
-
 ?
N-substituted aminoacyl-tRNA + H2O Bacillus cereus ATCC 14579
-
 N-substituted amino acid + tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus ATCC 14579
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel affinity column chromatography and Superdex 75 gel filtration Bacillus cereus

Storage Stability

Storage Stability Organism
22°C, 20 mM sodium acetate, 150 mM sodium chloride, 2 mM dithiothreitol, pH 5.0, several months, minimal loss of activity Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bulk peptidyl-tRNA + H2O
-
 Bacillus cereus ?
-
 ?
bulk peptidyl-tRNA + H2O
-
 Bacillus cereus ATCC 14579 ?
-
 ?
N-substituted aminoacyl-tRNA + H2O
-
 Bacillus cereus N-substituted amino acid + tRNA
-
 ?
N-substituted aminoacyl-tRNA + H2O
-
 Bacillus cereus ATCC 14579 N-substituted amino acid + tRNA
-
 ?

Synonyms

Synonyms Comment Organism
peptidyl-tRNA hydrolase 1
-
 Bacillus cereus
Pth1
-
 Bacillus cereus