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Literature summary for 3.1.1.29 extracted from

  • Vandavasi, V.; Taylor-Creel, K.; McFeeters, R.L.; Coates, L.; McFeeters, H.
    Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium (2014), Acta Crystallogr. Sect. F, 70, 872-877.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 pLysS cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.03 M citric acid, 0.05 M Bis-Tris propane, 1% (v/v) glycerol, 3% (w/v) sucrose, 25% (w/v) PEG 6000 pH 7.6 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium A0A0F6B281
-
-
Salmonella enterica subsp. enterica serovar Typhimurium 14028s A0A0F6B281
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating affinity column chromatography and Superose 12 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
22°C, 20 mM Bis-Tris pH 6.6, 50 mM NaCl, 2 mM dithiothreitol, several weeks, without loss of activity or precipitation Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidyl-tRNA + H2O
-
Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?
peptidyl-tRNA + H2O
-
Salmonella enterica subsp. enterica serovar Typhimurium 14028s ?
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
peptidyl-tRNA hydrolase
-
Salmonella enterica subsp. enterica serovar Typhimurium
Pth1
-
Salmonella enterica subsp. enterica serovar Typhimurium