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Literature summary for 3.1.1.29 extracted from

  • Giorgi, L.; Plateau, P.; O'Mahony, G.; Aubard, C.; Fromant, M.; Thureau, A.; Grotli, M.; Blanquet, S.; Bontems, F.
    NMR-based substrate analog docking to Escherichia coli peptidyl-tRNA hydrolase (2011), J. Mol. Biol., 412, 619-633.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain K37DELTArecAlambdaDE3 Escherichia coli

Protein Variants

Protein Variants Comment Organism
H20A the mutant is unable to hydrolyze 2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine Escherichia coli
N10A the mutant shows strongly reduced activity with diacetyl-lysyl-tRNALys and L-Lys-tRNALys compared to the wild type enzyme Escherichia coli
N10D the mutant shows strongly reduced activity with diacetyl-lysyl-tRNALys and L-Lys-tRNALys compared to the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3'-L-(N,N-diacetyl-lysinyl)amino-3'-deoxyadenosine
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7D1
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine + H2O minimalist substrate Escherichia coli ?
-
?
diacetyl-lysyl-tRNALys + H2O diacetyl-lysyl-tRNALys is hydrolyzed by the wild type enzyme 360fold more efficiently than Lys-tRNALys Escherichia coli diacetyl-lysine + tRNALys
-
?
L-Lys-tRNALys + H2O
-
Escherichia coli L-lysine + tRNALys
-
?

Synonyms

Synonyms Comment Organism
peptidyl-tRNA hydrolase
-
Escherichia coli
PTH
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
12
-
3'-L-(N,N-diacetyl-lysinyl)amino-3'-deoxyadenosine at pH 6.0, with 50 mM sodium acetate and 200 mM NaCl, at 28°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.51
-
2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine wild type enzyme, 28°C, 20 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM dithiothreitol Escherichia coli