Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain K37DELTArecAlambdaDE3 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H20A | the mutant is unable to hydrolyze 2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine | Escherichia coli |
N10A | the mutant shows strongly reduced activity with diacetyl-lysyl-tRNALys and L-Lys-tRNALys compared to the wild type enzyme | Escherichia coli |
N10D | the mutant shows strongly reduced activity with diacetyl-lysyl-tRNALys and L-Lys-tRNALys compared to the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-L-(N,N-diacetyl-lysinyl)amino-3'-deoxyadenosine | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7D1 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine + H2O | minimalist substrate | Escherichia coli | ? | - |
? | |
diacetyl-lysyl-tRNALys + H2O | diacetyl-lysyl-tRNALys is hydrolyzed by the wild type enzyme 360fold more efficiently than Lys-tRNALys | Escherichia coli | diacetyl-lysine + tRNALys | - |
? | |
L-Lys-tRNALys + H2O | - |
Escherichia coli | L-lysine + tRNALys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidyl-tRNA hydrolase | - |
Escherichia coli |
PTH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
3'-L-(N,N-diacetyl-lysinyl)amino-3'-deoxyadenosine | at pH 6.0, with 50 mM sodium acetate and 200 mM NaCl, at 28°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine | wild type enzyme, 28°C, 20 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM dithiothreitol | Escherichia coli |