Cloned (Comment) | Organism |
---|---|
gene pth, orf Rv1014c, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant C-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3) and in Escherichia coli thermosensitive strain AA7852, the mutant strain is able to grow at the nonpermissive temperature of 42°C, at 39°C, overexpression of MtPth in AA7852 cells allowed the cells to remain viable in the presence of up to 200 mg/ml erythromycin, overview | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C166A | site-directed mutagenesis, the mutant effectively complements the enzyme-defective thermosensitive Escherichia coli mutant strain AA7852 for growth at 42°C | Mycobacterium tuberculosis |
C67S | site-directed mutagenesis, the mutant effectively complements the enzyme-defective thermosensitive Escherichia coli mutant strain AA7852 for growth at 42°C | Mycobacterium tuberculosis |
D95N | site-directed mutagenesis, the catalytic residue mutant is not able to complement the enzyme-defective thermosensitive Escherichia coli mutant strain AA7852 for growth at 42°C | Mycobacterium tuberculosis |
H22N | site-directed mutagenesis, the catalytic residue mutant is not able to complement the enzyme-defective thermosensitive Escherichia coli mutant strain AA7852 for growth at 42°C | Mycobacterium tuberculosis |
H22N | site-directed mutagenesis, the mutation affects the enzyme structure, overview | Mycobacterium tuberculosis |
N12D | site-directed mutagenesis, the catalytic residue mutant is not able to complement the enzyme-defective thermosensitive Escherichia coli mutant strain AA7852 for growth at 42°C | Mycobacterium tuberculosis |
General Stability | Organism |
---|---|
urea/guanidinium chloride-induced unfolding curve for MtPth indicates a simple two-state unfolding process without any intermediates, pH 6.5, 25°C | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Mycobacterium tuberculosis | |
0.0007 | - |
diacetyl-lysyl-tRNALys | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21978 | - |
x * 21978, sequence calculation | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl-lysyl-tRNALys + H2O | Mycobacterium tuberculosis | - |
diacetyl-lysine + tRNALys | - |
? | |
diacetyl-lysyl-tRNALys + H2O | Mycobacterium tuberculosis H37Rv | - |
diacetyl-lysine + tRNALys | - |
? | |
additional information | Mycobacterium tuberculosis | the enzyme and its conserved active-site residues N12, H22 and D95 are essential for the viability of the bacteria | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | the enzyme and its conserved active-site residues N12, H22 and D95 are essential for the viability of the bacteria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene pth, orf Rv1014c | - |
Mycobacterium tuberculosis H37Rv | - |
gene pth, orf Rv1014c | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl-lysyl-tRNALys + H2O | - |
Mycobacterium tuberculosis | diacetyl-lysine + tRNALys | - |
? | |
diacetyl-lysyl-tRNALys + H2O | - |
Mycobacterium tuberculosis H37Rv | diacetyl-lysine + tRNALys | - |
? | |
additional information | the enzyme and its conserved active-site residues N12, H22 and D95 are essential for the viability of the bacteria | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme and its conserved active-site residues N12, H22 and D95 are essential for the viability of the bacteria | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21978, sequence calculation | Mycobacterium tuberculosis |
More | 3D fold based on NMR and a structural model based on the Escherichia coli Pth crystal structure are generated for Mycobacterium tuberculosis Pth, structure comparison, molecular modeling, construction of a model of structural changes associated with enzyme action on the basis of the plasticity of the molecule, overview | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
peptidyl-tRNA hydrolase | - |
Mycobacterium tuberculosis |
PTH | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermally-induced unfolding curve for MtPth indicates a simple two-state unfolding process without any intermediates, thermodynamic stability of the enzyme, pH 6.5, 25°C, overview | Mycobacterium tuberculosis |
52 | - |
stable up to | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.22 | - |
diacetyl-lysyl-tRNALys | - |
Mycobacterium tuberculosis |