Literature summary for 3.1.1.29 extracted from

Selvaraj, M.; Singh, N.S.; Roy, S.; Sangeetha, R.; Varshney, U.; Vijayan, M.
Cloning, expression, purification, crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis (2006), Acta Crystallogr. Sect. F, 62, 913-915.

Cloned(Commentary)

Cloned (Comment)	Organism
gene pth, orf Rv1014c, DNA and amino acid sequence determination, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme, microbatch method, 0.002 ml each of the protein solution, containing 6 mg/ml protein, 20 mM Tris-HCl, pH 7.5, 100 mM NaCl and 2 mM 2-mercaptoethanol, and the precipitant solution, containing 20% w/v PEG 8000 in 0.1 M HEPES, pH 7.5, and 5% v/v 2-propanol or dioxane or 25% w/v PEG 8000, 100 mM sodium cacodylate, pH 6.6, and 5% v/v 2-propanol, are mixed, 5 days to 2 weeks, X-ray diffraction structure determination and analysis at 1.97-2.49 A resolution	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

purified recombinant His-tagged enzyme, microbatch method, 0.002 ml each of the protein solution, containing 6 mg/ml protein, 20 mM Tris-HCl, pH 7.5, 100 mM NaCl and 2 mM 2-mercaptoethanol, and the precipitant solution, containing 20% w/v PEG 8000 in 0.1 M HEPES, pH 7.5, and 5% v/v 2-propanol or dioxane or 25% w/v PEG 8000, 100 mM sodium cacodylate, pH 6.6, and 5% v/v 2-propanol, are mixed, 5 days to 2 weeks, X-ray diffraction structure determination and analysis at 1.97-2.49 A resolution

Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20455	-	x * 20455, sequence calculation	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mycobacterium tuberculosis	the enzyme salvages tRNA from peptidyl-tRNA by hydrolyzing the ester link between the peptide and the 2'-or 3'-OH of the sugar at the end of tRNA, since accumulation of peptidyl-tRNA, due to drop-off of translating ribosomes, is toxic to the cell, overview	?	-	?
additional information	Mycobacterium tuberculosis H37Rv	the enzyme salvages tRNA from peptidyl-tRNA by hydrolyzing the ester link between the peptide and the 2'-or 3'-OH of the sugar at the end of tRNA, since accumulation of peptidyl-tRNA, due to drop-off of translating ribosomes, is toxic to the cell, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	gene pth, orf Rv1014c	-
Mycobacterium tuberculosis H37Rv	-	gene pth, orf Rv1014c	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme salvages tRNA from peptidyl-tRNA by hydrolyzing the ester link between the peptide and the 2'-or 3'-OH of the sugar at the end of tRNA, since accumulation of peptidyl-tRNA, due to drop-off of translating ribosomes, is toxic to the cell, overview	Mycobacterium tuberculosis	?	-	?
additional information	the enzyme salvages tRNA from peptidyl-tRNA by hydrolyzing the ester link between the peptide and the 2'-or 3'-OH of the sugar at the end of tRNA, since accumulation of peptidyl-tRNA, due to drop-off of translating ribosomes, is toxic to the cell, overview	Mycobacterium tuberculosis H37Rv	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 20455, sequence calculation	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
peptidyl-tRNA hydrolase	-	Mycobacterium tuberculosis
PTH	-	Mycobacterium tuberculosis