Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, 1.8 A resolution | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D86A | kcat/Km for diacetyl-lysyl-tRNALys is 0.22% of wild-type value | Saccharolobus solfataricus |
D86A/K18A | kcat/Km for diacetyl-lysyl-tRNALys is 0.17% of wild-type value | Saccharolobus solfataricus |
H25A | kcat/Km for diacetyl-lysyl-tRNALys is 13% of wild-type value | Saccharolobus solfataricus |
K118A | kcat/Km for diacetyl-lysyl-tRNALys is 87% of wild-type value | Saccharolobus solfataricus |
K18A | kcat/Km for diacetyl-lysyl-tRNALys is 0.2% of wild-type value | Saccharolobus solfataricus |
K56A | kcat/Km for diacetyl-lysyl-tRNALys is 12% of wild-type value | Saccharolobus solfataricus |
Q22A | kcat/Km for diacetyl-lysyl-tRNALys is 20% of wild-type value | Saccharolobus solfataricus |
Q54A | kcat/Km for diacetyl-lysyl-tRNALys is 47% of wild-type value | Saccharolobus solfataricus |
T90A | kcat/Km for diacetyl-lysyl-tRNALys is 1.4% of wild-type value | Saccharolobus solfataricus |
T98A | kcat/Km for diacetyl-lysyl-tRNALys is 34% of wild-type value | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme T98A | Saccharolobus solfataricus | |
1.6 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme Q22A | Saccharolobus solfataricus | |
2.9 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme Q54A | Saccharolobus solfataricus | |
3.2 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme K56A | Saccharolobus solfataricus | |
4.4 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme H25A | Saccharolobus solfataricus | |
5.3 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme K18A | Saccharolobus solfataricus | |
5.4 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme D86A | Saccharolobus solfataricus | |
6.7 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme D86A/K18A | Saccharolobus solfataricus | |
7.2 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme T90A | Saccharolobus solfataricus | |
9.1 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, wild-type enzyme | Saccharolobus solfataricus | |
12.8 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme K118A | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl-lysyl-tRNALys | - |
Saccharolobus solfataricus | diacetyl-Lys + tRNALys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YbaK | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme D86A/K18A | Saccharolobus solfataricus | |
0.002 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme K18A | Saccharolobus solfataricus | |
0.0025 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme D86A | Saccharolobus solfataricus | |
0.02 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme T90A | Saccharolobus solfataricus | |
0.06 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme Q22A | Saccharolobus solfataricus | |
0.06 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme T98A | Saccharolobus solfataricus | |
0.07 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme K56A | Saccharolobus solfataricus | |
0.1 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme H25A | Saccharolobus solfataricus | |
0.27 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme Q54A | Saccharolobus solfataricus | |
1.8 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, wild-type enzyme | Saccharolobus solfataricus | |
2.2 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, mutant enzyme K118A | Saccharolobus solfataricus |