Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deacetylated tRNA | - |
Artemia sp. | |
EDTA | 5 mM, complete inhibition | Artemia sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme is maximally active in presence of divalent cations, Mg2+ or Mn2+. Maximal activity with 20 mM Mg2+ | Artemia sp. | |
Mn2+ | the enzyme is maximally active in presence of divalent cations, Mg2+ or Mn2+. Maximal activity with 2.5 mM, 50% of the activation with Mg2+ | Artemia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Artemia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Artemia sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Artemia sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0149 | - |
- |
Artemia sp. |
Storage Stability | Organism |
---|---|
-10°C, 50% glycerol, purified enzyme is stable for several months | Artemia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glu-tRNA + H2O | - |
Artemia sp. | Glu + tRNA | - |
? | |
Gly-tRNA + H2O | - |
Artemia sp. | Gly + tRNA | - |
? | |
Leu-tRNA + H2O | - |
Artemia sp. | Leu + tRNA | - |
? | |
Lys-tRNA + H2O | - |
Artemia sp. | Lys + tRNA | - |
? | |
N-acetyl-Glu-tRNA + H2O | - |
Artemia sp. | N-acetyl-Glu + tRNA | - |
? | |
N-acetyl-Leu-tRNA + H2O | - |
Artemia sp. | N-acetyl-Leu + tRNA | - |
? | |
N-acetyl-Lys-tRNA + H2O | - |
Artemia sp. | N-acetyl-Lys + tRNA | - |
? | |
N-acetyl-Phe-tRNA + H2O | - |
Artemia sp. | N-acetyl-Phe + tRNA | - |
? | |
N-acetyl-Ser-tRNA + H2O | - |
Artemia sp. | N-acetyl-Ser + tRNA | - |
? | |
N-acetyl-Val-tRNA + H2O | - |
Artemia sp. | N-acetyl-Val + tRNA | - |
? | |
Phe-tRNA + H2O | - |
Artemia sp. | Phe + tRNA | - |
? | |
Ser-tRNA + H2O | - |
Artemia sp. | Ser + tRNA | - |
? | |
Val-tRNA + H2O | - |
Artemia sp. | Val + tRNA | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, 85% loss of activity | Artemia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Artemia sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | 4°C, 24 h, stable | Artemia sp. |