BRENDA - Enzyme Database
show all sequences of 3.1.1.29

Peptidyl-tRNA hydrolase from Sulfolobus solfataricus

Fromant, M.; Ferri-Fioni, M.L.; Plateau, P.; Blanquet, S.; Nucleic Acids Res. 31, 3227-3235 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
spermidine
0.1 mM spermidine HCl, 73fold activation
Saccharolobus solfataricus
spermidine-HCl
0.1 mM, 73fold activation
Saccharolobus solfataricus
spermidine-HCl
activates about 80fold, optimally at 0.1 mM
Saccharolobus solfataricus
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Saccharolobus solfataricus
gene SS00175, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deficient Escherichia coli mutant strain, and of two Saccharomyces cerevisiae gene YHR189w or YBL057c disruption mutants, overview, expression of PTH in Escherichia coli strain XL 1-Blue
Saccharolobus solfataricus
Inhibitors
Inhibitors
Commentary
Organism
Structure
non-esterified tRNALys
-
Saccharolobus solfataricus
tRNA
unfractionated Escherichia coli tRNA; unfractionated from Escherichia coli
Saccharolobus solfataricus
tRNA-formyl-methionine
from Escherichia coli
Saccharolobus solfataricus
tRNA-lysine
from Escherichia coli
Saccharolobus solfataricus
tRNAfMet
tRNAfMet from Escherichia coli
Saccharolobus solfataricus
tRNALys
tRNALys from Escherichia coli
Saccharolobus solfataricus
Uncharged tRNA
e.g. from Escherichia coli, at concentrations of 0.01 mM or above
Saccharolobus solfataricus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0000028
-
diacetyl-Lys-tRNALys
pH 7.5, 50C, dephosphorylated diacetyl-Lys-tRNALys
Saccharolobus solfataricus
0.0000028
-
diacetyl-lysyl-tRNALys
dephosphorylated substrate, pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.000003
-
dephosphorylated diacyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.000011
-
diacetyl-Lys-tRNALys
pH 7.5, 50C
Saccharolobus solfataricus
0.000011
-
diacetyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.000011
-
diacetyl-lysyl-tRNALys
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.000012
-
formyl-Met-tRNAfMet
pH 7.5, 50C
Saccharolobus solfataricus
0.000012
-
formyl-methionyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.000012
-
formyl-methionyl-tRNAfMet
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.000016
-
acetyl-His-tRNAHis
pH 7.5, 50C
Saccharolobus solfataricus
0.000016
-
acetyl-histidyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.000016
-
acetyl-histidyl-tRNAHis
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.00003
-
dephosphorylated formyl-methioninyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.00003
-
dephosphorylated formyl-Met-tRNAfMet
pH 7.5, 50C, dephosphorylated formyl-Met-tRNAfMet
Saccharolobus solfataricus
0.00003
-
formyl-methionyl-tRNAfMet
dephosphorylated substrate, pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
0.3 mM, 77fold activation
Saccharolobus solfataricus
KCl
300 mM, 77fold activation
Saccharolobus solfataricus
KCl
activates about 80fold, optimally at 300 mM
Saccharolobus solfataricus
Mg2+
40 mM MgCl2, about 88fold activation
Saccharolobus solfataricus
Mg2+
activates about 80fold, optimally at 40 mM
Saccharolobus solfataricus
MgCl2
40 mM, approx. 88fold activation
Saccharolobus solfataricus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
13100
-
2 * 13100, deduced from nucleotide sequence
Saccharolobus solfataricus
15000
-
x * 15000, SDS-PAGE
Saccharolobus solfataricus
15000
-
2 * 15000, recombinant enzyme, SDS-PAGE
Saccharolobus solfataricus
25000
-
gel filtration
Saccharolobus solfataricus
25000
-
recombinant enzyme, gel filtration
Saccharolobus solfataricus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
diacetyl-lysyl-tRNALys + H2O
Saccharolobus solfataricus
-
diacetyl-lysine + tRNALys
-
-
?
N-substituted aminoacyl-tRNA + H2O
Escherichia coli
-
N-substituted amino acid + tRNA
-
Escherichia coli
?
N-substituted aminoacyl-tRNA + H2O
Saccharolobus solfataricus
-
N-substituted amino acid + tRNA
-
Saccharolobus solfataricus
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus
-
gene SSO0175
-
Saccharomyces cerevisiae
-
YHR189w and YBL057c gene products
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Escherichia coli
-
Saccharolobus solfataricus
recombinant PTH, heat, ammonium sulfate, Superdex 75, HI-Propyl, SP-Sepharose
Saccharolobus solfataricus
recomninant PTH from Escherichia coli strain XL 1-Blue 8500fold by ultracentrifugation, heat treatment, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, and ion exchange chromatography to homogeneity
Saccharolobus solfataricus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Saccharolobus solfataricus
0.078
-
substrate D-tyrosyl-tRNA, 37C, pH 7.5
Saccharolobus solfataricus
0.15
-
substrae D-tyrosyl-tRNA
Escherichia coli
4.14
-
-
Saccharolobus solfataricus
4.14
-
purified recombinant enzyme
Saccharolobus solfataricus
30
-
substrate diacetyl-lysine-tRNA
Escherichia coli
31.8
-
substrate diacetyl-lysine tRNA, 37C, pH 7.5
Saccharolobus solfataricus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5'-dephosphorylated N,N-diacetyl-L-lysyl-tRNALys + H2O
-
648112
Saccharolobus solfataricus
N,N-diacetyl-L-lysine + 5'-dephosphorylated tRNALys
-
648112
Saccharolobus solfataricus
?
5'-dephosporylated N-formyl-L-methionyl-tRNA + H2O
-
648112
Saccharolobus solfataricus
N-formyl-L-methionine + 5'-dephosphorylated tRNA
-
648112
Saccharolobus solfataricus
?
acetyl-histidine-tRNA + H2O
-
648112
Saccharolobus solfataricus
acetyl-histidine + tRNA
-
648112
Saccharolobus solfataricus
?
acetyl-histidyl-tRNAHis + H2O
-
648112
Saccharolobus solfataricus
acetyl-histidine + tRNAHis
-
-
-
?
D-tyrosine-tRNA + H2O
-
648112
Escherichia coli
D-tyrosine + tRNA
-
648112
Escherichia coli
?
D-tyrosine-tRNA + H2O
-
648112
Saccharolobus solfataricus
D-tyrosine + tRNA
-
648112
Saccharolobus solfataricus
?
dephosphorylated diacyl-lysine-tRNA + H2O
-
648112
Saccharolobus solfataricus
dephosphorylated diacyl-lysine + tRNA
-
-
-
?
dephosphorylated formyl-Met-tRNAfMet + H2O
-
648112
Saccharolobus solfataricus
dephosphorylated formyl-Met + tRNAfMet
-
-
-
?
dephosphorylated formyl-methioninyl-tRNA + H2O
-
648112
Saccharolobus solfataricus
dephosphorylated formyl-methionine + tRNA
-
-
-
?
diacetyl-Lys-tRNALys + H2O
-
648112
Saccharomyces cerevisiae
diacetyl-Lys + tRNA
-
-
-
?
diacetyl-Lys-tRNALys + H2O
diacetyl-Lys-tRNALys from E. coli
648112
Saccharolobus solfataricus
diacetyl-Lys + tRNA
-
-
-
?
diacetyl-lysine-tRNA + H2O
-
648112
Escherichia coli
diacetyl-lysine + tRNA
-
648112
Escherichia coli
?
diacetyl-lysine-tRNA + H2O
-
648112
Saccharolobus solfataricus
diacetyl-lysine + tRNA
-
648112
Saccharolobus solfataricus
?
diacetyl-lysyl-tRNALys + H2O
-
648112
Saccharolobus solfataricus
diacetyl-lysine + tRNALys
-
-
-
?
diacetyl-lysyl-tRNALys + H2O
Escherichia coli diacetyl-lysyl-tRNALys, phosphorylated and dephosphorylated substrate
648112
Saccharolobus solfataricus
diacetyl-lysine + tRNALys
-
-
-
?
formyl-Met-tRNAfMet + H2O
-
648112
Saccharolobus solfataricus
formyl-Met + tRNAfMet
-
-
-
?
formyl-methionine-tRNA + H2O
-
648112
Saccharolobus solfataricus
formyl-methionine + tRNA
-
648112
Saccharolobus solfataricus
?
formyl-methioninyl-tRNA + H2O
-
648112
Saccharolobus solfataricus
formyl-methionine + tRNA
-
-
-
?
formyl-methionyl-tRNAfMet + H2O
Escherichia coli formyl-methionyltRNAfMet, phosphorylated and dephosphorylated substrate
648112
Saccharolobus solfataricus
formyl-methionine + tRNAfMet
-
-
-
?
additional information
the enzyme is involved in the recycling of peptidyl-tRNA, it shows poor D-aminoacyl-tRNA hydrolysis
648112
Saccharolobus solfataricus
?
-
-
-
?
N-acetyl-His-tRNA + H2O
-
648112
Saccharolobus solfataricus
N-acetyl-His + tRNA
-
-
-
?
N-substituted aminoacyl-tRNA + H2O
-
648112
Escherichia coli
N-substituted amino acid + tRNA
-
648112
Escherichia coli
?
N-substituted aminoacyl-tRNA + H2O
-
648112
Saccharolobus solfataricus
N-substituted amino acid + tRNA
-
648112
Saccharolobus solfataricus
?
Subunits
Subunits
Commentary
Organism
?
x * 15000, SDS-PAGE
Saccharolobus solfataricus
dimer
2 * 13100, deduced from nucleotide sequence
Saccharolobus solfataricus
dimer
2 * 15000, recombinant enzyme, SDS-PAGE
Saccharolobus solfataricus
Synonyms
Synonyms
Commentary
Organism
peptidyl-tRNA hydrolase
-
Saccharolobus solfataricus
PTH
-
Escherichia coli
PTH
-
Saccharolobus solfataricus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
assay at
Saccharolobus solfataricus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.86
-
diacetyl-Lys-tRNALys
pH 7.5, 50C, dephosphorylated diacetyl-Lys-tRNALys
Saccharolobus solfataricus
0.86
-
dephosphorylated diacyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.86
-
diacetyl-lysyl-tRNALys
dephosphorylated substrate, pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
1.8
-
diacetyl-Lys-tRNALys
pH 7.5, 50C
Saccharolobus solfataricus
1.8
-
diacetyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
1.8
-
diacetyl-lysyl-tRNALys
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
3
-
formyl-Met-tRNAfMet
pH 7.5, 50C
Saccharolobus solfataricus
3
-
formyl-methionyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
3
-
dephosphorylated formyl-methioninyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
3
-
formyl-methioninyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
3
-
formyl-methionyl-tRNAfMet
phosphorylated and dephosphorylated substrate, pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
3.4
-
acetyl-His-tRNA
pH 7.5, 50C
Saccharolobus solfataricus
3.4
-
acetyl-histidyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
3.4
-
acetyl-histidyl-tRNAHis
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
6.08
-
dephosphorylated diacyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Saccharolobus solfataricus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00005
-
tRNALys
pH 7.5, 50C, tRNALys from Escherichia coli
Saccharolobus solfataricus
0.00005
-
tRNA-lysine
tRNA-lysine from Escherichia coli, 50C, pH 7.5
Saccharolobus solfataricus
0.00005
-
non-esterified tRNALys
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.000095
-
tRNA
pH 7.5, 50C, unfractionated Escherichia coli tRNA
Saccharolobus solfataricus
0.000095
-
tRNA
unfractionated tRNA from Escherichia coli, 50C, pH 7.5
Saccharolobus solfataricus
0.0001
-
tRNA-formyl-methionine
tRNA-ormyl-methionine from Escherichia coli, 50C, pH 7.5
Saccharolobus solfataricus
0.0001
-
Uncharged tRNA
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.00011
-
tRNAformylMet
pH 7.5, 50C, tRNAformylMet from Escherichia coli
Saccharolobus solfataricus
0.00011
-
tRNAfMet
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
spermidine
0.1 mM spermidine HCl, 73fold activation
Saccharolobus solfataricus
spermidine-HCl
0.1 mM, 73fold activation
Saccharolobus solfataricus
spermidine-HCl
activates about 80fold, optimally at 0.1 mM
Saccharolobus solfataricus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Saccharolobus solfataricus
gene SS00175, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deficient Escherichia coli mutant strain, and of two Saccharomyces cerevisiae gene YHR189w or YBL057c disruption mutants, overview, expression of PTH in Escherichia coli strain XL 1-Blue
Saccharolobus solfataricus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
non-esterified tRNALys
-
Saccharolobus solfataricus
tRNA
unfractionated Escherichia coli tRNA
Saccharolobus solfataricus
tRNA
unfractionated from Escherichia coli
Saccharolobus solfataricus
tRNA-formyl-methionine
from Escherichia coli
Saccharolobus solfataricus
tRNA-lysine
from Escherichia coli
Saccharolobus solfataricus
tRNAfMet
-
Saccharolobus solfataricus
tRNAfMet
tRNAfMet from Escherichia coli
Saccharolobus solfataricus
tRNALys
tRNALys from Escherichia coli
Saccharolobus solfataricus
Uncharged tRNA
e.g. from Escherichia coli, at concentrations of 0.01 mM or above
Saccharolobus solfataricus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00005
-
tRNALys
pH 7.5, 50C, tRNALys from Escherichia coli
Saccharolobus solfataricus
0.00005
-
tRNA-lysine
tRNA-lysine from Escherichia coli, 50C, pH 7.5
Saccharolobus solfataricus
0.00005
-
non-esterified tRNALys
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.000095
-
tRNA
pH 7.5, 50C, unfractionated Escherichia coli tRNA
Saccharolobus solfataricus
0.000095
-
tRNA
unfractionated tRNA from Escherichia coli, 50C, pH 7.5
Saccharolobus solfataricus
0.0001
-
tRNA-formyl-methionine
tRNA-ormyl-methionine from Escherichia coli, 50C, pH 7.5
Saccharolobus solfataricus
0.0001
-
Uncharged tRNA
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.00011
-
tRNAformylMet
pH 7.5, 50C, tRNAformylMet from Escherichia coli
Saccharolobus solfataricus
0.00011
-
tRNAfMet
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0000028
-
diacetyl-Lys-tRNALys
pH 7.5, 50C, dephosphorylated diacetyl-Lys-tRNALys
Saccharolobus solfataricus
0.0000028
-
diacetyl-lysyl-tRNALys
dephosphorylated substrate, pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.000003
-
dephosphorylated diacyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.000011
-
diacetyl-Lys-tRNALys
pH 7.5, 50C
Saccharolobus solfataricus
0.000011
-
diacetyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.000011
-
diacetyl-lysyl-tRNALys
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.000012
-
formyl-Met-tRNAfMet
pH 7.5, 50C
Saccharolobus solfataricus
0.000012
-
formyl-methionyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.000012
-
formyl-methionyl-tRNAfMet
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.000016
-
acetyl-His-tRNAHis
pH 7.5, 50C
Saccharolobus solfataricus
0.000016
-
acetyl-histidyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.000016
-
acetyl-histidyl-tRNAHis
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
0.00003
-
dephosphorylated formyl-methioninyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.00003
-
dephosphorylated formyl-Met-tRNAfMet
pH 7.5, 50C, dephosphorylated formyl-Met-tRNAfMet
Saccharolobus solfataricus
0.00003
-
formyl-methionyl-tRNAfMet
dephosphorylated substrate, pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
0.3 mM, 77fold activation
Saccharolobus solfataricus
KCl
300 mM, 77fold activation
Saccharolobus solfataricus
KCl
activates about 80fold, optimally at 300 mM
Saccharolobus solfataricus
Mg2+
40 mM MgCl2, about 88fold activation
Saccharolobus solfataricus
Mg2+
activates about 80fold, optimally at 40 mM
Saccharolobus solfataricus
MgCl2
40 mM, approx. 88fold activation
Saccharolobus solfataricus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
13100
-
2 * 13100, deduced from nucleotide sequence
Saccharolobus solfataricus
15000
-
x * 15000, SDS-PAGE
Saccharolobus solfataricus
15000
-
2 * 15000, recombinant enzyme, SDS-PAGE
Saccharolobus solfataricus
25000
-
gel filtration
Saccharolobus solfataricus
25000
-
recombinant enzyme, gel filtration
Saccharolobus solfataricus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
diacetyl-lysyl-tRNALys + H2O
Saccharolobus solfataricus
-
diacetyl-lysine + tRNALys
-
-
?
N-substituted aminoacyl-tRNA + H2O
Escherichia coli
-
N-substituted amino acid + tRNA
-
Escherichia coli
?
N-substituted aminoacyl-tRNA + H2O
Saccharolobus solfataricus
-
N-substituted amino acid + tRNA
-
Saccharolobus solfataricus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
-
Saccharolobus solfataricus
recombinant PTH, heat, ammonium sulfate, Superdex 75, HI-Propyl, SP-Sepharose
Saccharolobus solfataricus
recomninant PTH from Escherichia coli strain XL 1-Blue 8500fold by ultracentrifugation, heat treatment, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, and ion exchange chromatography to homogeneity
Saccharolobus solfataricus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Saccharolobus solfataricus
0.078
-
substrate D-tyrosyl-tRNA, 37C, pH 7.5
Saccharolobus solfataricus
0.15
-
substrae D-tyrosyl-tRNA
Escherichia coli
4.14
-
-
Saccharolobus solfataricus
4.14
-
purified recombinant enzyme
Saccharolobus solfataricus
30
-
substrate diacetyl-lysine-tRNA
Escherichia coli
31.8
-
substrate diacetyl-lysine tRNA, 37C, pH 7.5
Saccharolobus solfataricus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5'-dephosphorylated N,N-diacetyl-L-lysyl-tRNALys + H2O
-
648112
Saccharolobus solfataricus
N,N-diacetyl-L-lysine + 5'-dephosphorylated tRNALys
-
648112
Saccharolobus solfataricus
?
5'-dephosporylated N-formyl-L-methionyl-tRNA + H2O
-
648112
Saccharolobus solfataricus
N-formyl-L-methionine + 5'-dephosphorylated tRNA
-
648112
Saccharolobus solfataricus
?
acetyl-histidine-tRNA + H2O
-
648112
Saccharolobus solfataricus
acetyl-histidine + tRNA
-
648112
Saccharolobus solfataricus
?
acetyl-histidyl-tRNAHis + H2O
-
648112
Saccharolobus solfataricus
acetyl-histidine + tRNAHis
-
-
-
?
D-tyrosine-tRNA + H2O
-
648112
Escherichia coli
D-tyrosine + tRNA
-
648112
Escherichia coli
?
D-tyrosine-tRNA + H2O
-
648112
Saccharolobus solfataricus
D-tyrosine + tRNA
-
648112
Saccharolobus solfataricus
?
dephosphorylated diacyl-lysine-tRNA + H2O
-
648112
Saccharolobus solfataricus
dephosphorylated diacyl-lysine + tRNA
-
-
-
?
dephosphorylated formyl-Met-tRNAfMet + H2O
-
648112
Saccharolobus solfataricus
dephosphorylated formyl-Met + tRNAfMet
-
-
-
?
dephosphorylated formyl-methioninyl-tRNA + H2O
-
648112
Saccharolobus solfataricus
dephosphorylated formyl-methionine + tRNA
-
-
-
?
diacetyl-Lys-tRNALys + H2O
-
648112
Saccharomyces cerevisiae
diacetyl-Lys + tRNA
-
-
-
?
diacetyl-Lys-tRNALys + H2O
diacetyl-Lys-tRNALys from E. coli
648112
Saccharolobus solfataricus
diacetyl-Lys + tRNA
-
-
-
?
diacetyl-lysine-tRNA + H2O
-
648112
Escherichia coli
diacetyl-lysine + tRNA
-
648112
Escherichia coli
?
diacetyl-lysine-tRNA + H2O
-
648112
Saccharolobus solfataricus
diacetyl-lysine + tRNA
-
648112
Saccharolobus solfataricus
?
diacetyl-lysyl-tRNALys + H2O
-
648112
Saccharolobus solfataricus
diacetyl-lysine + tRNALys
-
-
-
?
diacetyl-lysyl-tRNALys + H2O
Escherichia coli diacetyl-lysyl-tRNALys, phosphorylated and dephosphorylated substrate
648112
Saccharolobus solfataricus
diacetyl-lysine + tRNALys
-
-
-
?
formyl-Met-tRNAfMet + H2O
-
648112
Saccharolobus solfataricus
formyl-Met + tRNAfMet
-
-
-
?
formyl-methionine-tRNA + H2O
-
648112
Saccharolobus solfataricus
formyl-methionine + tRNA
-
648112
Saccharolobus solfataricus
?
formyl-methioninyl-tRNA + H2O
-
648112
Saccharolobus solfataricus
formyl-methionine + tRNA
-
-
-
?
formyl-methionyl-tRNAfMet + H2O
Escherichia coli formyl-methionyltRNAfMet, phosphorylated and dephosphorylated substrate
648112
Saccharolobus solfataricus
formyl-methionine + tRNAfMet
-
-
-
?
additional information
the enzyme is involved in the recycling of peptidyl-tRNA, it shows poor D-aminoacyl-tRNA hydrolysis
648112
Saccharolobus solfataricus
?
-
-
-
?
N-acetyl-His-tRNA + H2O
-
648112
Saccharolobus solfataricus
N-acetyl-His + tRNA
-
-
-
?
N-substituted aminoacyl-tRNA + H2O
-
648112
Escherichia coli
N-substituted amino acid + tRNA
-
648112
Escherichia coli
?
N-substituted aminoacyl-tRNA + H2O
-
648112
Saccharolobus solfataricus
N-substituted amino acid + tRNA
-
648112
Saccharolobus solfataricus
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 15000, SDS-PAGE
Saccharolobus solfataricus
dimer
2 * 13100, deduced from nucleotide sequence
Saccharolobus solfataricus
dimer
2 * 15000, recombinant enzyme, SDS-PAGE
Saccharolobus solfataricus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
assay at
Saccharolobus solfataricus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.86
-
diacetyl-Lys-tRNALys
pH 7.5, 50C, dephosphorylated diacetyl-Lys-tRNALys
Saccharolobus solfataricus
0.86
-
dephosphorylated diacyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
0.86
-
diacetyl-lysyl-tRNALys
dephosphorylated substrate, pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
1.8
-
diacetyl-Lys-tRNALys
pH 7.5, 50C
Saccharolobus solfataricus
1.8
-
diacetyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
1.8
-
diacetyl-lysyl-tRNALys
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
3
-
formyl-Met-tRNAfMet
pH 7.5, 50C
Saccharolobus solfataricus
3
-
formyl-methionyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
3
-
dephosphorylated formyl-methioninyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
3
-
formyl-methioninyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
3
-
formyl-methionyl-tRNAfMet
phosphorylated and dephosphorylated substrate, pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
3.4
-
acetyl-His-tRNA
pH 7.5, 50C
Saccharolobus solfataricus
3.4
-
acetyl-histidyl-tRNA
50C, pH 7.5
Saccharolobus solfataricus
3.4
-
acetyl-histidyl-tRNAHis
pH 7.5, 50C, recombinant enzyme
Saccharolobus solfataricus
6.08
-
dephosphorylated diacyl-lysine-tRNA
50C, pH 7.5
Saccharolobus solfataricus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Saccharolobus solfataricus
Other publictions for EC 3.1.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749860
Kaushik
Search of multiple hot spots ...
Acinetobacter baumannii, Acinetobacter baumannii AB307-0294
Biochem. J.
475
547-560
2018
-
-
1
1
-
-
1
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749977
Shahid
Role of methionine 71 in subs ...
Vibrio cholerae serotype O1, Vibrio cholerae serotype O1 El Tor Inaba N16961, Vibrio cholerae serotype O1 ATCC 39315
Biochim. Biophys. Acta
1866
865-874
2018
-
-
1
1
1
-
-
-
-
-
-
3
-
5
-
-
1
-
-
-
-
-
3
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
751694
Verma
Vms1 and ANKZF1 peptidyl-tRNA ...
Saccharomyces cerevisiae, Homo sapiens, Saccharomyces cerevisiae ATCC 204508
Nature
557
446-451
2018
-
-
2
-
2
-
-
-
-
-
-
3
-
5
-
-
2
-
-
-
-
-
6
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
752162
Kabra
Unraveling the stereochemical ...
Vibrio cholerae serotype O1
RNA
23
202-216
2017
-
-
-
1
5
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
3
3
-
-
-
749971
Kabra
Structural characterization o ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084, Mycolicibacterium smegmatis mc(2)155
Biochim. Biophys. Acta
1864
1304-1314
2016
-
-
1
-
-
-
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
3
1
4
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
752098
Burks
Expression, purification, and ...
Homo sapiens
Protein Expr. Purif.
126
49-54
2016
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
749483
Zhang
Crystal structure of Staphylo ...
Staphylococcus aureus, Staphylococcus aureus NCTC 8325
Acta Biochim. Biophys. Sin. (Shanghai)
47
1005-1010
2015
-
-
1
1
-
-
-
-
-
-
1
2
-
12
-
-
1
-
-
-
-
-
4
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
728921
Vandavasi
Recombinant production, crysta ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium 14028s
Acta Crystallogr. Sect. F
70
872-877
2014
-
-
1
1
-
-
-
-
-
-
1
-
-
13
-
-
1
-
-
-
-
1
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729170
Singh
Structural and binding studies ...
Pseudomonas aeruginosa
Biochem. J.
463
329-337
2014
-
-
1
1
-
-
2
-
-
-
1
-
-
8
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729311
Sharma
Structural and functional insi ...
Acinetobacter baumannii, Escherichia coli, Francisella tularensis, Mycobacterium tuberculosis, Mycolicibacterium smegmatis, Pseudomonas aeruginosa, Saccharolobus solfataricus, Pyrococcus horikoshii, Methanocaldococcus jannaschii, Saccharolobus solfataricus P2, Pyrococcus horikoshii OT-3
Biochim. Biophys. Acta
1844
1279-1288
2014
-
-
-
-
-
-
-
-
-
-
-
11
-
53
-
-
-
-
-
-
-
-
22
9
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
22
9
-
-
-
-
-
-
-
-
-
9
9
-
-
-
730882
Taylor-Creel
Expression, purification, and ...
Bacillus cereus, Bacillus cereus ATCC 14579
Protein Expr. Purif.
95
259-264
2014
-
-
1
-
-
-
-
1
-
-
-
2
-
13
-
-
1
-
-
-
-
1
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728907
Matsumoto
Crystallization and preliminar ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Acta Crystallogr. Sect. F
69
332-335
2013
-
-
1
1
-
-
-
-
-
-
-
2
-
94
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728976
Goedeke
Recycling of peptidyl-tRNAs by ...
Pseudomonas aeruginosa
Antimicrob. Agents Chemother.
57
1617-1624
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
8
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730350
Dujeancourt
Interactions between peptidyl ...
Schizosaccharomyces pombe
Mitochondrion
13
871-880
2013
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730738
Kaushik
The mode of inhibitor binding ...
Acinetobacter baumannii
PLoS ONE
8
e67547
2013
-
-
1
1
-
-
-
-
-
-
-
1
-
8
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728894
Selvaraj
Structures of new crystal form ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. F
68
124-128
2012
-
-
1
1
-
-
-
-
-
-
-
1
-
6
-
-
1
-
-
-
-
-
2
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729836
Kumar
Crystal structure of peptidyl- ...
Mycolicibacterium smegmatis
Int. J. Biochem. Mol. Biol.
3
58-69
2012
-
-
1
1
-
-
-
-
-
-
1
1
-
8
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730449
McFeeters
Inhibition of essential bacter ...
Escherichia coli
Nat. Prod. Commun.
7
1107-1110
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
730485
Ito
Structural basis for the subst ...
Escherichia coli
Nucleic Acids Res.
40
10521-10531
2012
-
-
1
1
3
-
-
4
-
-
-
1
-
3
-
-
1
-
-
-
-
-
2
-
2
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
715942
Giorgi
NMR-based substrate analog doc ...
Escherichia coli
J. Mol. Biol.
412
619-633
2011
-
-
1
-
3
-
1
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
1
-
-
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708331
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A functional peptidyl-tRNA hyd ...
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690236
Shimizu
Structure of peptidyl-tRNA hyd ...
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Acta Crystallogr. Sect. D
64
444-453
2008
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1
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Pulavarti
Solution structure and dynamic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
378
165-177
2008
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1
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710503
Singarapu
NMR structure of the peptidyl- ...
Pseudomonas syringae pv. tomato
Proteins Struct. Funct. Genet.
71
1027-1031
2008
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1
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1
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678535
Bal
Characterization of peptidyl-t ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
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467-479
2007
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1
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6
1
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1
4
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1
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4
2
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2
1
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Selvaraj
Structural plasticity and enzy ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
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186-193
2007
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4
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4
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721151
Fukunaga
Structure of the AlaX-M trans- ...
Pyrococcus horikoshii
Acta Crystallogr. Sect. D
63
390-400
2007
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1
1
1
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2
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1
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2
1
3
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1
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2
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1
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2
1
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677362
Selvaraj
Cloning, expression, purificat ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
62
913-915
2006
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2
1
2
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1
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1
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2
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1
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2
1
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681789
Das
Peptidyl-tRNA hydrolase and it ...
Escherichia coli
Microbiology
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2191-2195
2006
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1
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1
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9
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1
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3
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3
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682160
Vivanco-Dominguez
Excess of charged tRNALys main ...
Escherichia coli
Nucleic Acids Res.
34
1564-1570
2006
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1
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8
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1
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2
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2
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1
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1
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1
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2
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664102
Fromant
-
Crystal structure at 1.8 A res ...
Saccharolobus solfataricus
Biochemistry
44
4292-4301
2005
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1
10
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11
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1
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1
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1
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1
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11
-
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1
10
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11
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1
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1
-
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11
-
-
-
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666859
Powers
Solution structure of Archaegl ...
Archaeoglobus fulgidus
Protein SCi.
14
2849-2861
2005
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-
-
-
-
-
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20
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1
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678132
Fromant
Crystal structure at 1.8 A res ...
Saccharolobus solfataricus
Biochemistry
44
4294-4301
2005
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1
1
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15
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1
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1
2
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1
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1
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1
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-
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-
650249
Goodall
Essential role of histidine 20 ...
Escherichia coli
Biochemistry
43
4583-4591
2004
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1
1
12
-
1
1
-
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1
-
4
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1
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-
-
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2
-
1
-
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1
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1
-
1
12
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1
-
1
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1
-
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1
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-
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2
-
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1
-
-
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666482
Singh
A physiological connection bet ...
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Nucleic Acids REs.
32
6028-2037
2004
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1
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1
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4
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1
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1
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1
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1
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648110
de Pereda
Crystral structure of a human ...
Escherichia coli, Saccharolobus solfataricus, Homo sapiens
J. Biol. Chem.
279
8111-8115
2003
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2
2
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1
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8
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3
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4
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2
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2
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3
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3
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1
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8
-
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4
-
-
-
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648112
Fromant
Peptidyl-tRNA hydrolase from S ...
Saccharomyces cerevisiae, Escherichia coli, Saccharolobus solfataricus
Nucleic Acids Res.
31
3227-3235
2003
3
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2
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7
15
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6
5
3
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14
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4
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-
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7
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23
3
3
1
-
-
15
1
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-
-
9
-
-
3
-
2
-
-
-
-
-
9
9
15
-
6
5
3
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-
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4
-
-
7
-
23
3
1
-
-
15
1
-
-
-
-
-
-
-
-
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648116
Rosas-Sandoval
Orthologs of a novel archaeal ...
Methanocaldococcus jannaschii
Proc. Natl. Acad. Sci. USA
99
16707-16712
2002
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1
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2
1
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19
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1
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5
2
1
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1
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-
-
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2
1
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-
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1
-
-
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5
2
-
-
-
-
-
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-
-
-
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-
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653271
Menez
Peptidyl-tRNA hydrolase in Bac ...
Bacillus subtilis, Saccharomyces cerevisiae
Mol. Microbiol.
45
123-129
2002
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2
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1
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2
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21
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2
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3
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2
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1
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2
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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653741
Bonin
Expression, purification, and ...
Staphylococcus aureus
Protein Expr. Purif.
24
123-130
2002
-
-
1
-
-
-
-
1
-
1
1
1
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6
-
-
1
-
-
-
1
-
2
1
2
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
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653153
Menez
Suppression of thermosensitive ...
Escherichia coli
Microbiology
147
1581-1589
2001
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-
-
-
-
-
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1
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1
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6
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-
-
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2
-
2
-
-
-
-
-
-
-
-
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-
-
-
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-
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1
-
-
1
-
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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648118
Schmitt
Crystal structure at 1.2 A res ...
Escherichia coli
EMBO J.
16
4760-4769
1997
-
-
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1
7
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7
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8
-
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1
-
-
-
-
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1
-
-
-
-
-
7
-
-
-
-
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-
-
-
-
-
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1
7
-
-
-
-
7
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1
-
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1
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7
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648119
Schmitt
Crystallization and preliminar ...
Escherichia coli
Proteins Struct. Funct. Genet.
28
135-136
1997
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1
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6
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1
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-
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-
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648117
Dutka
Role of the 1-72 base pair in ...
Escherichia coli
Nucleic Acids Res.
21
4025-4030
1993
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-
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2
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6
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1
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4
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2
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1
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2
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1
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1
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4
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2
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1
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648115
Refugio Garcia-Villegas
Peptidyl-tRNA hydrolase is inv ...
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EMBO J.
10
3549-3555
1991
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1
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8
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1
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1
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1
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648114
Ferreiro
-
Purification and characterizat ...
Fusarium culmorum
Biochim. Biophys. Acta
882
410-418
1986
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2
2
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1
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1
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1
1
17
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1
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1
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1
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2
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2
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1
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1
17
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1
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1
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648113
Gallego
-
Purification and properties of ...
Artemia sp.
Biochim. Biophys. Acta
696
57-65
1982
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13
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1
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1
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1
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2
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2
1
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1
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1
1
1
13
-
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1
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1
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1
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648111
Miralles
Independent temporal expressio ...
Artemia salina
Biochim. Biophys. Acta
518
326-333
1978
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1
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1
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648109
Neth
Hydrolase activity for N-subst ...
Homo sapiens, Rattus norvegicus, Rattus norvegicus Wistar
Hoppe-Seyler's Z. Physiol. Chem.
353
117-121
1972
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1
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12
-
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1
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648108
Vogel
The protection by 70 S ribosom ...
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Eur. J. Biochem.
21
582-592
1971
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1
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1
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1
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1
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1
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648107
Jost
Enzymatic hydrolysis of N-subs ...
Saccharomyces cerevisiae
J. Biol. Chem.
244
5866-5873
1969
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1
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3
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1
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1
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7
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1
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1
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3
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1
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1
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1
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1
7
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1
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648106
Paulin
Enzymatic hydrolysis of N-subs ...
Escherichia coli, Escherichia coli MRE 600
FEBS Lett.
1
163-165
1968
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27
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16
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16
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