Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
full length human Pth2 clone as tempolate , subcloned into the NcoI/BamHI sites of the pET15b bacterial expression vector. The ligated plasmid is transfoirmed into Escherichia coli BL21(DE3) strain | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
recombinant enzyme, sitting drop vapor diffusion techniques | Homo sapiens |
sitting-drop vapor diffusion at room temperature, 10 mg/ml protein in 100 mM HEPES, pH 7.5 and 20% polyethylene glycol 10000 is equilibrated against a reservoir of the same solution, crystals diffract to 2.0 A | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000011 | - |
diacetyl-lysine-tRNA | 27°C, pH 7.5 | Saccharolobus solfataricus | |
0.00022 | - |
diacetyl-lysine-tRNA | 27°C, pH 7.5 | Homo sapiens | |
0.006 | - |
diacetyl-lysine-tRNA | 27°C, pH 7.5 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-substituted aminoacyl-tRNA + H2O | Escherichia coli | - |
N-substituted amino acid + tRNA | - |
? | |
N-substituted aminoacyl-tRNA + H2O | Saccharolobus solfataricus | - |
N-substituted amino acid + tRNA | - |
? | |
N-substituted aminoacyl-tRNA + H2O | Homo sapiens | - |
N-substituted amino acid + tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | Q9Y3E5 | - |
- |
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Pth | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl-Lys-tRNA + H2O | - |
Homo sapiens | diacetyl-Lys + tRNA | - |
? | |
diacetyl-Lys-tRNALys + H2O | - |
Homo sapiens | diacetyl-Lys + tRNA | - |
? | |
diacetyl-lysine-tRNA + H2O | - |
Escherichia coli | diacetyl-lysine + tRNA | - |
? | |
diacetyl-lysine-tRNA + H2O | - |
Saccharolobus solfataricus | diacetyl-lysine + tRNA | - |
? | |
diacetyl-lysine-tRNA + H2O | - |
Homo sapiens | diacetyl-lysine + tRNA | - |
? | |
N-substituted aminoacyl-tRNA + H2O | - |
Escherichia coli | N-substituted amino acid + tRNA | - |
? | |
N-substituted aminoacyl-tRNA + H2O | - |
Saccharolobus solfataricus | N-substituted amino acid + tRNA | - |
? | |
N-substituted aminoacyl-tRNA + H2O | - |
Homo sapiens | N-substituted amino acid + tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTH | - |
Escherichia coli |
Pth2 | - |
Saccharolobus solfataricus |
Pth2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
diacetyl-Lys-tRNA | pH 7.5, 27°C | Homo sapiens | |
1.3 | - |
diacetyl-lysine-tRNA | 27°C, pH 7.5 | Homo sapiens | |
1.8 | - |
diacetyl-lysine-tRNA | 27°C, pH 7.5 | Saccharolobus solfataricus | |
3.6 | - |
diacetyl-lysine-tRNA | 27°C, pH 7.5 | Escherichia coli |