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Literature summary for 3.1.1.27 extracted from

  • Funami, J.; Yoshikane, Y.; Kobayashi, H.; Yokochi, N.; Yuan, B.; Iwasaki, K.; Ohnishi, K.; Yagi, T.
    4-Pyridoxolactonase from a symbiotic nitrogen-fixing bacterium Mesorhizobium loti: cloning, expression, and characterization (2005), Biochim. Biophys. Acta, 1753, 234-239.
    View publication on PubMed
Search for more literature for 3.1.1.27

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mesorhizobium loti

General Stability

General Stability Organism
enzyme shows very low activity in Tris-HCl bufffers Mesorhizobium loti

Inhibitors

Inhibitors Comment Organism Structure
5-Pyridoxolactone competitive Mesorhizobium loti
Hg2+ 0.1 mM, complete inhibition Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 4-Pyridoxolactone pH 7.5 Mesorhizobium loti
0.319
-
 4-pyridoxate pH 7.5 Mesorhizobium loti

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ one mole per mole of subunit, tightly bound to the protein Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29855
-
 2 * 31000, SDS-PAGE, 2 * 29855, calculated Mesorhizobium loti
31000
-
 2 * 31000, SDS-PAGE, 2 * 29855, calculated Mesorhizobium loti
59000
-
 gel filtration Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti
-
 overexpression in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-pyridoxate
-
 Mesorhizobium loti 4-pyridoxolactone + H2O
-
 r
4-pyridoxolactone + H2O
-
 Mesorhizobium loti 4-pyridoxate
-
 r
N-hexanoyl-D,L-homoserine lactone + H2O 41% of the activity with 4-pyridoxolactone Mesorhizobium loti N-hexanoyl-D,L-homoserine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31000, SDS-PAGE, 2 * 29855, calculated Mesorhizobium loti
additional information N-terminal amino acid sequence Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
 Mesorhizobium loti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, stable Mesorhizobium loti
55
-
 10 min, 64% residual activity Mesorhizobium loti
60
-
 10 min, 14% residual activity Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 4-pyridoxate pH 7.5 Mesorhizobium loti
12.2
-
 4-Pyridoxolactone pH 7.5 Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Mesorhizobium loti

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 36% of maximum activity Mesorhizobium loti
9.5
-
 10% of maximum activity Mesorhizobium loti

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.15
-
 5-Pyridoxolactone pH 7.5 Mesorhizobium loti