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Literature summary for 3.1.1.26 extracted from
- Purification and properties of galactolipase (1969), Biochim. Biophys. Acta, 178, 519-533.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 45% inhibition between 2-10 mM | Phaseolus coccineus |  |
| cysteine | 50% inhibition between 2-10 mM | Phaseolus coccineus | |
| dithioerythritol | 13% inhibition with 13 mM | Phaseolus coccineus | |
| glutathione | reduced, 48% inhibition 5-10 mM | Phaseolus coccineus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
digalactosyldiacylglycerol | - |
Phaseolus coccineus | |
| 0.65 | - |
monogalactosyldiacylglycerol | - |
Phaseolus coccineus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Sodium dithionite | highest activity between 4-7 mM, above decrease of activation | Phaseolus coccineus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
gel filtration | Phaseolus coccineus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digalactosyldiacylglycerol + H2O | Phaseolus coccineus | - |
? | - |
? | |
| monogalactosyldiacylglycerol + H2O | Phaseolus coccineus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus coccineus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phaseolus coccineus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, (NH4)2SO4-precipitation at pH-optimum, after 4 weeks 53.6% of the original activity left | Phaseolus coccineus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digalactosyldiacylglycerol + H2O | - |
Phaseolus coccineus | digalactosylglycerol + fatty acid | - |
? | |
| digalactosyldiacylglycerol + H2O | - |
Phaseolus coccineus | ? | - |
? | |
| monogalactosyldiacylglycerol + H2O | - |
Phaseolus coccineus | galactosylglycerol + fatty acid | - |
? | |
| monogalactosyldiacylglycerol + H2O | - |
Phaseolus coccineus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
70-75% of the activity left after 10 min, monogalacatosyldiacylglycerol as substrate, 16% of the activity left after 10 min with digalactosyldiacylglycerol as substrate | Phaseolus coccineus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 10 | cysteine | - |
Phaseolus coccineus | |
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